Regulation of the protein and gene expressions of ethylene biosynthesis enzymes under different temperature during peach fruit ripening
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Ethylene has profound effect on fruit development and ripening, and the role of ethylene biosynthesis enzymes involving 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS), ACC oxidase (ACO), and S-Adenosyl-l-methionine synthetase (SAMS) in peach fruit (cv. Xiahui-8) was characterized under 25 and 4 °C, respectively. All these enzymes in ethylene synthesis pathway were identified using 2-DE and real-time PCR. Both protein and gene expressions of ACO and SAMS were much higher at 25 °C than at 4 °C. Among five members of ACS family, PpaACS4 may belong to system II ethylene biosynthesis, while PpaACS3 involved in system I during development stage, and low temperature can induce PpaACS1 expression. The ethylene release and low expressions of proteins and genes of most enzymes indicated that low temperature can effectively postpone ripening stage by reducing ethylene evolution. High gene expression of PpaSAMS did not cause excessive expression of SAMS protein under low temperature, and over-expression of PpaACS1 at low temperature still did not induce increase of ethylene production. The mechanism underlying the phenomenon about how temperature affects ethylene release was also discussed.
KeywordsPeach fruit Ripening Ethylene production ACC synthase ACC oxidase SAM synthetase
Author contribution statement
Author ZY designed the experiment, and XW conducted the experiment and wrote this paper. CH, MY, and RM were participated in part of this experiment and provided some guidance. ZY helped with further revision to the manuscript. All authors read and approved the final version of the manuscript.
- Crisosto CH, Day KR, Crisosto GM, Garner D (2001) Quality attributes of white flesh peaches and nectarines grown under California conditions. J Amer Pomolog Soc 55:45–51Google Scholar
- Mbeguie-A-Mbeguie D, Chahine H, Gomez RM, Gouble B, Reich M, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B (1999) Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca). Physiol Plantarum 105:294–303CrossRefGoogle Scholar
- Sis SA, Mostofi Y, Boojar MMA, Khalighi A (2012) Effect of nitric oxide on ethylene biosynthesis and antioxidant enzymes on Iranian peach (Prunus persica cv. Anjiri). J Food Agric Environ 10:125–129Google Scholar
- Torrigiani P, Bressanin D, Ruiz KB, Tadiello A, Trainotti L, Bonghi C, Ziosi V, Costa G (2012) Spermidine application to young developing peach fruits leads to a slowing down of ripening by impairing ripening-related ethylene and auxin metabolism and signaling. Physiol Plantarum 146:86–98CrossRefGoogle Scholar