Establishment of culturing conditions and assessment of antioxidant activity and somaclonal variation in the adventitious root suspension cultures of Oplopanax elatus Nakai

  • Bimal-Kumar Ghimire
  • Hee-Young Kim
  • Eun-Soo Seong
  • Ji-Hye Yoo
  • Ji-Hee Lee
  • Sung-Kyu Park
  • Seung-Hyun Kim
  • Chang-Yeon Yu
  • Ill-Min Chung
Original Article


Oplopanax elatus Nakai, a plant traditionally used in folk medicine, is currently in population decline due to uncontrolled harvesting. In the present study, we investigated the factors affecting O. elatus adventitious root production, including hormones (alone or in combination), explant type, basal salt type and strength, sucrose concentration, pH, and temperature. Results revealed that adventitious root formation was optimal with root explants grown on 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L−1 Indole-3-butyric acid (IBA) (pH 5.8) at 25 °C. Chlorogenic acid concentration was highest in roots propagated in 1/2 MS medium containing 0.5 mg L−1 IBA; vanillin, another phenolic compound, was also detected in cultures. Liquid media containing 3% sucrose exhibited the highest radical scavenging activity and total phenolic compound contents. X-ray diffraction revealed significant differences in the elemental intensity between adventitious root and field-grown plantlet extracts. Analysis of simple sequence repeats confirmed that adventitious roots regenerated in vitro were genetically similar to their mother plant. Thus, we identified the optimal conditions for proliferation of O. elatus adventitious roots in liquid culture, from which, secondary metabolites, particularly bioactive compounds associated with the medicinal use of this plant, can be mass produced without further population deterioration.


Adventitious roots High performance liquid chromatography Oplopanax elatus Secondary metabolites Suspension cultures Simple sequence repeats 



This study was sponsored by the KU Brain Pool program of 2015, Konkuk University, South Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Agullo-Anton MA, Sanchez-bravo J, Acosta M, Druege U (2011) Auxins or sugars: what makes the difference in the adventitious rooting of stored carnation cuttings? J. Plant Grow. Regul 30:100–113CrossRefGoogle Scholar
  2. Al-Khayri JM, Al-Bahrany AM (2002) Callus growth and proline accumulation in response to sorbitol and sucrose-induced osmotic stress in rice. Biol Plant 45:609–611CrossRefGoogle Scholar
  3. Baque MA, Shiragi MHK, Moh SH, Lee EJ, Paek KY (2013) Production of biomass and bioactive compounds by adventitious root suspension cultures of Morinda citrifolia (L.) in a liquid-phase airlift balloon-type bioreactor. In Vitro Cell Dev Biol Plant 49:737–749CrossRefGoogle Scholar
  4. Bennett MD, Johnston S, Hodnett GL, Price HJ (2000) Allium cepa L. cultivars from four continents compared by flow cytometry show nuclear DNA constancy. Ann Bot 85:351–357CrossRefGoogle Scholar
  5. Bhatia P, Ashwath N (2005) Effect of medium pH on shoot regeneration from the cotyledonary explants of tomato. Biotechnology 4:7–10CrossRefGoogle Scholar
  6. Billotte N, Risterucci AM, Barcelos E, Noyer JL, Amblard P, Baurens FC (2001) Development, characterization and across-taxa utility of oil palm (Elaeis guineensis Jacq.) microsatellite markers. Genome 44:413–425CrossRefPubMedGoogle Scholar
  7. Blanca P, Bonfil M, Cruz-Hernandez A, Lopez-Laredo AR, Trejo-Tapia G, Trejo-Espino JL (2014) Effects of culture medium and auxins on growth of adventitious root cultures of Cuphea aequipetala Cav. and their ability to produce antioxidant compounds. Plant Cell Tissue Org Cult 118:401–408CrossRefGoogle Scholar
  8. Chan LK, Dewi PR, Boey PL (2005) Effect of plant growth regulators on regeneration of plantlets from bud cultures of Cymbopogon nardus L. and the detection of essential oils from the in vitro plantlets. J Plant Biol 48:142–145CrossRefGoogle Scholar
  9. Chao WJ, Serpe MD, Anderson RW, Gesch RW, Horvath DP (2006) Sugars, hormones, environment affect the dormancy status in underground adventitious buds of leaf spurge (Euphorbia esula). Weed Sci 54(1):59–68CrossRefGoogle Scholar
  10. Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101:708–4716CrossRefGoogle Scholar
  11. Cui HY, Baque MA, Lee EJ, Paek KY (2013) Scale-up of adventitious root cultures of Echinacea angustifolia in a pilot scale bioreactor for the production of biomass and caffeic acid derivatives. Plant Biotechnol Rep 7:97–308CrossRefGoogle Scholar
  12. Debnath SC (2005) Effects of carbon source and concentration on development of lingonberry (Vaccinium vitis-idaea L.) shoots cultivated in in vitro from nodal explants. In vitro Cell Dev Biol Plant 41:145–150CrossRefGoogle Scholar
  13. Dolezel J, Sgorbati S, Lucretti S (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiol Plant 85:625–631CrossRefGoogle Scholar
  14. Dornenburg H, Knorr D (1995) Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme Microb Technol 17:674–684CrossRefGoogle Scholar
  15. Dou DQ, Hu XY, Zhao YR, Kang TG, Liu FY, Kuang HX, Smith DC (2009) Studies on the anti-psoriasis constituents of Oplopanax elatus Nakai. Nat Prod Res 23:334–342CrossRefPubMedGoogle Scholar
  16. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
  17. Echeverrigaray S, Mossi AJ, Munari F (1998) Micropropagation of raisin tree (Hovenia dulcis Thunb.) through axillary bud culture. J Plant Biochem Biotechnol 7:99–102CrossRefGoogle Scholar
  18. Evans DE, Coleman JOD, Kearns A (2003) Callus cultures. In: Basics plant tissue culture. BIOS Scientific Publishers, Taylor and Francis group, New York, pp 63–67Google Scholar
  19. Fazal H, Abbasi BH, Ahmad N, Ali M, Ali S (2015) Sucrose induced osmotic stress and photoperiod regimes enhanced the biomass and production of antioxidant secondary metabolites in shake-flask suspension cultures of Prunella vulgaris L. Plant Cell Tissue Org Cult 124:573–581CrossRefGoogle Scholar
  20. Gao X, Zhu C, Jia W, Gao W, Qiu M, Zhang Y, Xiao P (2005) Induction and characterization of adventitious roots directly from leaf explants of Panax notoginseng. Biotechnol Lett 27:1771–1775CrossRefPubMedGoogle Scholar
  21. Ghimire BK, Yu CY, Chung IM (2017) Assessment of the phenolic profile, antimicrobial activity and oxidative stability of transgenic Perilla frutescens L. overexpressing tocopherol methyltransferase (g-tmt) gene. Plant Physiol Biochem 118:77–87CrossRefPubMedGoogle Scholar
  22. Hansen J (1989) Influence of cutting position and temperature during rooting on adventitious root formation and axillary bud break of Stephanotis floribunda. Scientia Hortic. 40:345–354CrossRefGoogle Scholar
  23. Hasan N, Hussein S, Ibrahim R (2014) Plant growth regulator effect on adventitious roots induction of Labisia pumila Mal. J Fundam Appl Sci 10:48–52Google Scholar
  24. Kaeppler SM, Kaepler HF, Rhee Y (2000) Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol 43:179–188CrossRefPubMedGoogle Scholar
  25. Kawakami H, Hara K, Komine M, Yamamoto Y (2015) Production of secoiridoids by adventitious root culture of Swertia japonica. Vitro Cell Dev Biol Plant 51:524–529CrossRefGoogle Scholar
  26. Khan MA, Abbasi BH, Ali H, Ali M, Adil M, Hussain I (2015a) Temporal variations in metabolite profiles at different growth phases during somatic embryogenesis of Silybum marianum L. Plant Cell Tiss Org Cult 120:127–139CrossRefGoogle Scholar
  27. Khan MA, Abbasi BH, Shah NA, Yucesan B, Ali H (2015b) Analysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal plant. Plant Cell Tissue Org Cult 123:501–510CrossRefGoogle Scholar
  28. Kim YJ, Wyslouzili BE, Weathers PJ (2002) Invited review: secondary metabolism of hairy root cultures in bioreactors. Vitro Cell Dev Biol Plant 38:1–10CrossRefGoogle Scholar
  29. Kim JH, Eom SH, Lee HS, Kim JK, Yu CY, Kwon YS, Lee JK, Kim MJ (2007) Assessment on antioxidant properties of Oplopanax elatus Nakai in vitro. Korean J Med Crop Sci 15:112–119Google Scholar
  30. Kwon HS, Kim DH, Shin HK, Yu CY, Kim MJ, Lim JD, Park JK, Kim JK (2007) Fourteen-day repeated-dose oral toxicity study of the ethanol extracts isolated from Oplopanax elatus in sprague-dawley rat. Korean J Food Sci Technol 39:470–475Google Scholar
  31. Kwon SJ, Cho KY, Kim HH (2014) Medium composition and growth regulator on organogenesis Platycodon grandiflorum (Jacq.) A. DC. with yellow green petals. Korean J Plant Res 27:43–50CrossRefGoogle Scholar
  32. Lee SW, Kim YM, Kim WW, Chung JM (2002) Genetic variation of I-SSR markers in the natural populations of a rare and endangered tree species, Oplopanax elatus in Korea. J Korean For Soc 91:565–573Google Scholar
  33. Lee EJ, Mobin ME, Hahn EJ, Paek KY (2006) Effects of sucrose, inoculums density, auxins and aerator volume on cell growth of Gymmema sylvestre. J Plant Biol 49:427–431CrossRefGoogle Scholar
  34. Lee JG, Seong ES, Goh EJ, Kim NY, Yu CY (2009) Factors involved in mass propagation of Ginseng (Panax ginseng C. A. Meyer) using bioreactor system. J Korean Soc Appl Biol Chem 52:466–471CrossRefGoogle Scholar
  35. Lee EJ, Park SY, Paek KY (2015) Enhancement strategies of bioactive compound production in adventitious root cultures of Eleutherococcus koreanum Nakai subjected to methyl jasmonate and salicylic acid elicitation through airlift bioreactors. Plant Cell Tissue Org Cult 120:1–10CrossRefGoogle Scholar
  36. Lian ML, Chakrabarty D, Paek KY (2002) Effect of plant growth regulators and medium composition on cell growth and saponin production during cell-suspension culture of mountain ginseng (Panax ginseng C.A. Mayer). J Plant Biol 45:201–206CrossRefGoogle Scholar
  37. Ling APK (2009) Kai Ming Kok K.M.; Hussein S.; Ong S.L. Effects of plant growth regulators on adventitious roots induction from different explants of Orthosiphon Stamineus. Am Eurasian J Sustain Agric 3(3):493–501Google Scholar
  38. Linkins AE, Lewis LN, Palmer RL (1973) Hormonally induced changes in the stem and petiole anatomy and cellulase enzyme patterns in Phaseolus vulgaris L. Plant Physiol 52:554–560CrossRefPubMedPubMedCentralGoogle Scholar
  39. Liu PP, Qu Y, Dou DQ, Kang TG, Smith D (2012) Determination of anti-cancer constituents in Oplopanax horridus and Oplopanax elatus. J Chem Soc Pak 34:417–423Google Scholar
  40. Ljung K, Nemhauser JL, Perata P (2015) New mechanistic links between sugar and hormone signalling networks. Curr Opin Plant Biol 25:130–137CrossRefPubMedGoogle Scholar
  41. Luschnig C (2002) Auxin transport: ABC proteins join the club. Trends Plant Sci 7(8):329–332CrossRefPubMedGoogle Scholar
  42. Manuhara YSW, Kristanti AN, Utami ESW, Yachya A (2015) Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. hairy root in balloon-type bubble bioreactor. Asian Pac J Trop Biomed 5:1027–1032CrossRefGoogle Scholar
  43. Maria GO, Alena G, Emilia O, Miroslava L, Gabriela L (2010) Effect of medium pH on axillary shoot proliferation of selected Vaccinium vitisidaea L. cultivars. Botanica 52:92–96Google Scholar
  44. McClung CRM (2001) Circadian rhythms in plants. Annu Rev Plant Physiol Mol Biol 52:139–162CrossRefGoogle Scholar
  45. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 7:109–114CrossRefGoogle Scholar
  46. Murthy HN, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotechnol 24:711–716CrossRefGoogle Scholar
  47. Murthy HM, Lee EJ, Paek KY (2014) Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tissue Org Cult 118:1–16CrossRefGoogle Scholar
  48. Narayan MS, Thimmaraju R, Bhagyalakshmi N (2005) Interplay of growth regulators during solid-state and liquid-state batch cultivation of anthocyanin producing cell line of Daucus carota. Process Biochem 40:351–358CrossRefGoogle Scholar
  49. Nookaraju A, Agrawal DC (2012) Genetic homogeneity of in vitro raised plants of grapevine cv. Crimson Seedless revealed by ISSR and microsatellite markers. S Afr J Bot 78:302–306CrossRefGoogle Scholar
  50. Park YY, Cho MS, Chung JB (2007) Effect of salt strength, sucrose concentration and NH4/NO3 ratio of medium on the shoot growth of Wasabia japonica in vitro culture. J Plant Biotechnol 34:263–269CrossRefGoogle Scholar
  51. Park SY, Moon HK, Murthy HN, Kim YW (2011) Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture. Biol Plant 55:559–562CrossRefGoogle Scholar
  52. Pasko P, Barton H, Zagrodzki P, Gorinstein S, Folta M, Zachwieja Z (2009) Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth. Food Chem 115:994–998CrossRefGoogle Scholar
  53. Peng X, Zhang TT, Zhang J (2015) Effect of subculture times on genetic fidelity, endogenous hormone level and pharmaceutical potential of Tetrastigma hemsleyanum callus. Plant Cell Tissue Org Cult 122:67–77CrossRefGoogle Scholar
  54. Perilli S, Moubayidin L, Sabatini S (2010) The molecular basis of cytokinin function. Curr Opin Plant Biol 13:21–26CrossRefPubMedGoogle Scholar
  55. Pop TI, Pamfil D, Bellini C (2011) Auxin control in the formation of adventitious roots. Not Bot Hortic Agrobot Cluj-Napoca 39(1):307–316Google Scholar
  56. Price AH, Atherton NM, Hendry GAF (1989) Plants under droughtstress generate activated oxygen. Free Rad Res Commun 8:61–66CrossRefGoogle Scholar
  57. Rajesh M, Sivanandhan G, Arun M, Vasudevan V, Theboral J, Girija S, Manickavasagam M, Selvaraj N, Ganapathi A (2014) Factors influencing podophyllotoxin production in adventitious root culture of Podophyllum hexandrum Royle. Acta Physiol Plant 36:1009–1021CrossRefGoogle Scholar
  58. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237CrossRefPubMedGoogle Scholar
  59. Riasat R, Zertashia Riasat Z, Abbasi BH, Liu C, Khan MA (2015) Silybum marianum: adventitious roots induction along with free radical scavenging activity. J Plant Biol Res 4:12–21Google Scholar
  60. Ricci A, Rolli E, Brunoni F, Dramis L, Sacco E, Fattorini L, Ruffoni B, Díaz-Sala C, Altamura MM (2016) 1,3-di(benzo[d]oxazol-5-yl)urea acts as either adventitious rooting adjuvant or xylogenesis enhancer in carob and pine microcuttings depending on the presence/absence of exogenous indole-3-butyric acid. Plant Cell Tissue Org Cult 126:411–427CrossRefGoogle Scholar
  61. Roitsch T, Bittner M, Godt DE (1995) Induction of apoplastic invertase of Chenopodium rubrum by d-glucose and a glucose analog and tissue-specific expression suggest a role in sinksource regulation. Plant Physiol 108:285–294CrossRefPubMedPubMedCentralGoogle Scholar
  62. Ruiz-Ramírez A, Chávez-Salgado M, Peñeda-Flores JA, Zapata E, Masso F, El-Hafidi M (2011) High-sucrose diet increases ROS generation, FFA accumulation, UCP2 level, and proton leak in liver mitochondria. Am J Physiol Endocrinol Metab 301(6):1198–1207CrossRefGoogle Scholar
  63. Sandnes JM, Kallqvist T, Wenner D, Gislerod HR (2005) Combined influence of light and temperature on growth rates of Nannochloropsis oceanica: linking cellular responses to large-scale biomass production. J Appl Phycol 17:515–525CrossRefGoogle Scholar
  64. Schenk RU, Hildebrandt AC (1972) Medium and techniques to induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204CrossRefGoogle Scholar
  65. Semalty A, Semalty M, Panda VS, Asrani KH, Ashar HD (2012) Herbal drugs in chronic fatigue syndrome: an overview. Schweiz Z Ganzheitsmed 24:155–168CrossRefGoogle Scholar
  66. Sheeja T, Mondal EA, Rathore PK (2004) Efficient plantlet regeneration in tomato (Lycopersicon esculentum Mill.). Plant Cell Tissue Org Cult 14:45–53Google Scholar
  67. Shinohara N, Sugiyama M, Fukuda H (2006) Higher extracellular pH suppresses tracheary element differentiation by affecting auxin uptake. Planta 224:394–404CrossRefPubMedGoogle Scholar
  68. Silveira JAG, Melo ARB, Viegas RA, Oliveira JTA (2001) Salinity-induced effects on nitrogen assimilation related to growth in cowpea plants. Environ Exp Bot 46:171–179CrossRefGoogle Scholar
  69. Simão MJ, Fonseca E, Garcia R, Mansur E, Pacheco G (2016) Effects of auxins and different culture systems on the adventitious root development of Passiflora pohlii Mast and their ability to produce antioxidant compounds. Plant Cell Tissue Org Cult 124:419–430CrossRefGoogle Scholar
  70. Sivanandhan G, Arun M, Mayavan S, Rajesh M, Mariashibu TS, Manickavasagam M, Selvaraj N, Ganapathi A (2012) Chitosan enhances withanolides production in adventitious root cultures of Withania somnifera (L.) Dunal. Ind Crops Prod 37:124–129CrossRefGoogle Scholar
  71. Slazak B, Sliwinska E, Saługa M, Ronikier M, Bujak J, Słomka A, Göransson U, Kuta E (2015) Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation enhanced cyclotide biosynthesis. Plant Cell Tissue Org Cult 120:179–190CrossRefGoogle Scholar
  72. Smeekens S (2000) Sugar-induced signal transduction in plants. Annu Rev Plant Physiol Plant Mol Biol 51:49–81CrossRefPubMedGoogle Scholar
  73. Solfanelli C, Poggi A, Loreti E, Alpi A, Perata P (2006) Sucrosespecific induction of the anthocyanin biosynthetic pathway in Arabidopsis. Plant Physiol 140:637–646CrossRefPubMedPubMedCentralGoogle Scholar
  74. Sorin C, Bussell JD, Camus I, Ljung K, Kowalczyk M, Geiss G, Mckhann H, Garcion C, Vaucheret H, Sandberg G, Bellini C (2005) Auxin and light control of adventitious rooting in Arabidopsis require ARGONAUTE1. Plant Cell 17:1343–1359CrossRefPubMedPubMedCentralGoogle Scholar
  75. Stingu A, Volf I, Popa VI, Gostin I (2012) New approaches concerning the utilization of natural amendments in cadmium phytoremediation. Ind Crops Prod 35:53–60CrossRefGoogle Scholar
  76. Tangahu BV, Abdullah SRS, Basri H, Idris M, Anuar N, Mukhlisin M (2011) A review on heavy metals (As, Pb, and Hg) uptake by plants through phytoremediation. Int J Chem 21(1–31):2011Google Scholar
  77. Thiruvengadam M, Chung IM (2015) Selenium, putrescine, and cadmium influence health-promoting phytochemicals and molecular-level effects on turnip (Brassica rapa ssp. rapa). Food Chem 173:185–193CrossRefPubMedGoogle Scholar
  78. Thiruvengadam M, Rekha K, Chung IM (2016) Induction of hairy roots by Agrobacterium rhizogenes-mediated transformation of spine gourd (Momordica dioica Roxb. ex. willd) for the assessment of phenolic compounds and biological activities. Sci Hortic 198:132–141CrossRefGoogle Scholar
  79. Tingey SV, del Tufo JP (1993) Genetic analysis with random amplified polymorphic DNA markers. Plant Physiol 101:349–352CrossRefPubMedPubMedCentralGoogle Scholar
  80. Turan M, Esringu A (2007) Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb and Zn. Plant Soil Environ 53:7–15CrossRefGoogle Scholar
  81. Viehmannova I, Cepkova PH, Vitamvas J, Streblova P, Jana Kisilova J (2016) Micropropagation of a giant ornamental bromeliad Puya berteroniana through adventitious shoots and assessment of their genetic stability through ISSR primers and flow cytometry. Plant Cell Tissue Org Cult 125:293–302CrossRefGoogle Scholar
  82. Vinterhalter B, Krstic-Milosevic D, Jankovic T, Pljevljakusic D, Ninkovic S, Smigocki A, Vinterhalter D (2015) Gentiana dinarica Beck. hairy root cultures and evaluation of factors affecting growth and xanthone production. Plant Cell Tissue Org Cult 121:667–679CrossRefGoogle Scholar
  83. Visser RG, Stolte A, Jacobsen E (1991) Expression of a chimaeric granule-bound starch synthase-GUS gene in transgenic potato plants. Plant Mol Biol 4:691–699CrossRefGoogle Scholar
  84. Vitrac X, Larronde F, Krisa S, Decendit A, Deffieux G, Merillon JM (2000) Sugar sensing and Ca2+–calmodulin requirement in Vitis vinifera cells producing anthocyanins. Phytochem 53:659–665CrossRefGoogle Scholar
  85. Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49:193–199CrossRefGoogle Scholar
  86. Wu SQ, Yu XK, Lian ML, Park SY, Piao XC (2014) Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors. Acta Physiol Plant 36:975–981CrossRefGoogle Scholar
  87. Yang MC, Kwon HC, Kim YJ, Lee KR, Yang HO (2010) Oploxynes A and B, polyacetylenes from the stems of Oplopanax elatus. J Nat Prod 73:801–805CrossRefPubMedGoogle Scholar
  88. Yin S, Gao W, Liang Y, Wang J, Liu H, Wei C, Zuo B (2013) Influence of sucrose concentration and phosphate source on biomass and metabolite accumulation in adventitious roots of Pseudostellaria heterophylla. Acta Physiol Plant 35:1579–1585CrossRefGoogle Scholar
  89. Zhang J, Gao WY, Wang J, Li XL (2011) Effects of explant types and media salt strength on growth and secondary metabolite accumulation in adventitious roots of Periploca sepium Bunge. Acta Physiol Plant 33:2447–2452CrossRefGoogle Scholar
  90. Zhang ZS, Yu ZY, Jin ZX, Liu J, Li YF (2013) Liquid culture of adventitious roots is a potential alternative to field cultivation for Psammosilene tunicoides, a rare and endangered endemic medicinal plant. Adv J Food Sci Technol 52:127–131CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Bimal-Kumar Ghimire
    • 1
  • Hee-Young Kim
    • 2
  • Eun-Soo Seong
    • 3
  • Ji-Hye Yoo
    • 2
  • Ji-Hee Lee
    • 1
  • Sung-Kyu Park
    • 1
  • Seung-Hyun Kim
    • 1
  • Chang-Yeon Yu
    • 2
  • Ill-Min Chung
    • 1
  1. 1.Department of Applied BioscienceKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Bioconvergence Science and Technology, College of Agriculture and Life ScienceKangwon National UniversityChuncheonRepublic of Korea
  3. 3.Department of Medicinal PlantSuwon Women’s UniversitySuwonRepublic of Korea

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