Establishment of culturing conditions and assessment of antioxidant activity and somaclonal variation in the adventitious root suspension cultures of Oplopanax elatus Nakai

  • Bimal-Kumar Ghimire
  • Hee-Young Kim
  • Eun-Soo Seong
  • Ji-Hye Yoo
  • Ji-Hee Lee
  • Sung-Kyu Park
  • Seung-Hyun Kim
  • Chang-Yeon Yu
  • Ill-Min Chung
Original Article
  • 19 Downloads

Abstract

Oplopanax elatus Nakai, a plant traditionally used in folk medicine, is currently in population decline due to uncontrolled harvesting. In the present study, we investigated the factors affecting O. elatus adventitious root production, including hormones (alone or in combination), explant type, basal salt type and strength, sucrose concentration, pH, and temperature. Results revealed that adventitious root formation was optimal with root explants grown on 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L−1 Indole-3-butyric acid (IBA) (pH 5.8) at 25 °C. Chlorogenic acid concentration was highest in roots propagated in 1/2 MS medium containing 0.5 mg L−1 IBA; vanillin, another phenolic compound, was also detected in cultures. Liquid media containing 3% sucrose exhibited the highest radical scavenging activity and total phenolic compound contents. X-ray diffraction revealed significant differences in the elemental intensity between adventitious root and field-grown plantlet extracts. Analysis of simple sequence repeats confirmed that adventitious roots regenerated in vitro were genetically similar to their mother plant. Thus, we identified the optimal conditions for proliferation of O. elatus adventitious roots in liquid culture, from which, secondary metabolites, particularly bioactive compounds associated with the medicinal use of this plant, can be mass produced without further population deterioration.

Keywords

Adventitious roots High performance liquid chromatography Oplopanax elatus Secondary metabolites Suspension cultures Simple sequence repeats 

Notes

Acknowledgements

This study was sponsored by the KU Brain Pool program of 2015, Konkuk University, South Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2018

Authors and Affiliations

  • Bimal-Kumar Ghimire
    • 1
  • Hee-Young Kim
    • 2
  • Eun-Soo Seong
    • 3
  • Ji-Hye Yoo
    • 2
  • Ji-Hee Lee
    • 1
  • Sung-Kyu Park
    • 1
  • Seung-Hyun Kim
    • 1
  • Chang-Yeon Yu
    • 2
  • Ill-Min Chung
    • 1
  1. 1.Department of Applied BioscienceKonkuk UniversitySeoulRepublic of Korea
  2. 2.Department of Bioconvergence Science and Technology, College of Agriculture and Life ScienceKangwon National UniversityChuncheonRepublic of Korea
  3. 3.Department of Medicinal PlantSuwon Women’s UniversitySuwonRepublic of Korea

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