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Acta Physiologiae Plantarum

, Volume 36, Issue 12, pp 3325–3331 | Cite as

Significant enhancement of lignan accumulation in hairy root cultures of Linum album using biotic elicitors

  • Sedigheh Esmaeilzadeh BahabadiEmail author
  • Mozafar Sharifi
  • Najme Ahmadian Chashmi
  • Jun Murata
  • Honoo Satake
Original Paper

Abstract

Linum album has been shown to accumulate some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, we examined the effects of fungal elicitors on the production of lignans in L. album hairy root cultures. The biosynthesis of lignans was differentially affected by fungal elicitors. Fusarium graminearum extract induced the highest increase of PTOX, 190 μg g−1 dry weight (DW), and lariciresinol, 260 μg g−1 DW, which was two-fold and three-fold greater than the untreated control, respectively, while Trichoderma viride extract enhanced the accumulation of MPTOX, instead of PTOX, up to 160 µg g−1 DW, which was 2.4-fold greater than the control. The enhancing effects of fungal elicitors on lignans production was correlated with the increased expression of some key genes involved in the biosynthesis of these compounds, phenylalanine ammonia-lyase, cinnamoyl-CoA reductase, cinnamyl-alcohol dehydrogenase and pinoresinol-lariciresinol reductase.

Keywords

Elicitation Hairy root Lignan Linum album 

Abbreviations

CAD

Cinnamyl-alcohol dehydrogenase

CCR

Cinnamoyl-CoA reductase

LC–ESI–MS/MS

Liquid chromatography–electrospray–mass spectrometry/mass spectrometry

MPTOX

6-Methoxy podophyllotoxin

PAL

Phenylalanine ammonia-lyase

PTOX

Podophyllotoxin

PLR

Pinoresinol-lariciresinol reductase

Notes

Acknowledgments

We are thankful to Tohru Yamagaki and Ryoichi Araki from Scientific and Technical Services of the Suntory Foundation for Life Sciences, Bioorganic Research Institute, for ESI–MS and qPCR analyses.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2014

Authors and Affiliations

  • Sedigheh Esmaeilzadeh Bahabadi
    • 1
    Email author
  • Mozafar Sharifi
    • 2
  • Najme Ahmadian Chashmi
    • 3
  • Jun Murata
    • 4
  • Honoo Satake
    • 4
  1. 1.Department of Biology, Faculty of Basic SciencesUniversity of ZabolZabolIran
  2. 2.Department of Plant Biology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  3. 3.Department of Biology, Faculty of Basic SciencesUniversity of MazandaranBabolsarIran
  4. 4.Suntory Foundation for Life SciencesBioorganic Research InstituteOsakaJapan

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