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Effects of different transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids, HSL and HMG-CoA reductase in hyperlipidemia rabbits

不同促透剂用于隔药饼灸对高脂血症兔肝脏脂质及HSL和HMG-CoA还原酶的影响

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Abstract

Objective

To observe the effects of laurocapram and borneol as transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids, hormone-sensitive lipase (HSL) and hydroxymethylglutaryl CoA (HMG-CoA) reductase in hyperlipidemia rabbits.

Methods

Forty New-Zealand rabbits were randomly divided into 5 groups using the random number table method, with 8 rats in each group. Rabbits in the blank group were fed routinely with a normal diet; rabbits in the other groups were fed with high-fat diet for 12 weeks to establish the hyperlipidemia model. Rabbits in the blank and the model groups were not given any intervention. After the model was prepared successfully, rabbits in the non-transdermal penetration enhancer group received herbal cake-partitioned moxibustion without transdermal penetration enhancers; rabbits in the laurocapram group and the borneol group received herbal cake-partitioned moxibustion with laurocapram or borneol respectively. After 4 weeks of treatment, the serum was isolated and enzyme-linked immunosorbent assay (ELISA) was applied for the detection of HSL and HMG-CoA reductase. The liver tissues were isolated, and total cholesterol (TC) and triglycerides (TG) were measured by enzymatic methods. One-step method was applied for high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) detection, and transmission turbidimetry was for apolipoprotein A1 (Apo-A1) and apolipoprotein B (Apo-B) detection.

Results

The serum concentrations of the drugs in the laurocapram and the borneol groups were significantly higher than those in the non-transdermal penetration enhancer group (both P<0.05); all drug penetrations in the borneol group were significantly higher than those in the laurocapram group (both P<0.05), except for tanshinone IIA. Compared with the non-transdermal penetration enhancer group, the HSL was significantly increased while the HMG-CoA reductase was significantly decreased in the laurocapram and the borneol groups (both P<0.05); between groups, the HSL in the borneol group was significantly higher than that in the laurocapram group (P<0.05). Compared with the blank group, the levels of LDL-C, TG, TC and Apo-B in rabbit liver were significantly increased in the model group (P<0.05); compared with the model group, the levels of LDL-C, TG, TC and Apo-B in the non-transdermal penetration enhancer, the laurocapram, and the borneol groups were significantly decreased (all P<0.05); between groups, the TG and TC in the laurocapram group and the LDL-C, TG, TC and Apo-B in the borneol group were significantly lower than those in the non-transdermal penetration enhancer group (all P<0.05), and the TG, LDL-C and Apo-B in the borneol group were significantly lower than those in the laurocapram group (all P<0.05). Compared with the blank group, the HDL-C and Apo-A1 were significantly decreased in the model group (both P<0.05), while compared with the model group, the HDL-C and Apo-A1 were significantly increased in the non-transdermal penetration enhancer, the laurocapram, and the borneol groups (all P<0.05). Between groups, the Apo-A1 in the laurocapram group, the HDL-C and Apo-A1 in the borneol group were significantly higher than those in the non-transdermal penetration enhancer group (all P<0.05).

Conclusion

The application of laurocapram and borneol, as transdermal penetration enhancers, in herbal cake-partitioned moxibustion can promote the penetration of the drugs in the herbal cake, increase the levels of HDL-C and Apo-A1, improve the metabolism of HSL and HMG-CoA reductase, and also simultaneously reduce the levels of TC, TG, LDL-C and Apo-B in the liver. The transdermal penetration enhancement effect of borneol is slightly better than or equivalent to that of laurocapram.

摘要

目的

探讨冰片和氮酮作为促透剂用于隔药饼灸对高脂血症兔肝脏脂质及激素敏感脂肪酶(HSL)和羟甲基戊二酰辅酶A(HMG-CoA)还原酶的影响.

方法

将40只新西兰兔按随机数字表法随机分成5组, 每组8只. 空白组正常喂养普通饲料; 其余组高脂饲料喂养12周, 复制高脂模型. 空白组和模型组不治疗. 无促透剂组成模后采用不加促透剂的药饼施灸; 氮酮组和冰片组分别将氮酮和冰片加于药饼中, 进行隔药饼灸. 治疗4周后, 分离血清, 酶联免疫吸附法(ELISA)检测HSL和HMG-CoA还原酶. 取肝组织采用酶法测定总胆固醇(TC)和甘油三酯(TG), 直接一步法测定高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C), 透射比浊法测定载脂蛋白A1(Apo-A1)及载脂蛋白B(Apo-B).

结果

氮酮组和冰片组血清药物浓度显著高于无促透剂组(均P<0.05); 除丹参酮IIA外, 冰片组其他药物渗透量均显著高于氮酮组(均P<0.05). 氮酮组和冰片组HSL显著高于无促透剂组, HMG-CoA还原酶显著低于无促透剂组(均P<0.05); 冰片组HSL显著高于氮酮组(P<0.05). 模型组兔肝脏LDL-C、 TG、 TC和Apo-B含量显著高于空白组(P<0.05); 无促透剂组、 氮酮组和冰片组LDL-C、 TG、 TC及Apo-B含量显著低于模型组(均P<0.05), 氮酮组TG和TC显著低于无促透剂组(均P<0.05), 冰片组LDL-C、 TG、 TC和Apo-B显著低于无促透剂组(均P<0.05); 冰片组TG、 LDL-C和Apo-B显著低于氮酮组(均P<0.05); 模型组HDL-C和Apo-A1显著低于空白组(均P<0.05), 无促透剂组、 氮酮组和冰片组HDL-C及Apo-A1显著高于模型组(均P<0.05), 氮酮组Apo-A1、 冰片组HDL-C及Apo-A1均显著高于无促透剂组(均P<0.05).

结论

氮酮和冰片作为促透剂用于隔药饼灸, 能促进药饼中药物吸收, 降低肝脏TC、TG、LDL-C和Apo-B含量, 提高HDL-C和Apo-A1含量, 改善HSL和HMG-CoA还原酶的代谢; 冰片效果略优于或相当于氮酮.

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Acknowledgments

This work was sponsored by Hunan Province College Innovation Open-end Fund Project (湖南省高校创新平台 开放基金项目, No. 16K068); 2017 Scientific and Research Innovative Project for Postgraduate Students of Hunan Province (2017 年湖南省研究生科研创新项目, No. CX2017B431); Scientific and Research Innovative Project for Undergraduate Students of Hunan Province (湖 南省大学生创新实验项目, No. 2017-132).

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Authors and Affiliations

  1. Hunan University of Chinese Medicine, Changsha, 410208, China

    Zong-li Liao  (廖宗力), Jing Tan  (谭静), Lu Sun  (孙璐), Yan-ping Chen  (陈艳萍), Ren-da Yang  (阳仁达) & Xiao-rong Chang  (常小荣)

  2. Xiniujiao Community Health Service Station, Jingxi Street, Baiyun District in Guangzhou City, Guangdong, 510515, China

    Zong-li Liao  (廖宗力)

  3. Huizhou Hospital of Traditional Chinese Medicine, Guangdong, 516000, China

    Chong-zheng Zhu  (朱重政)

  4. Department of Stomach Surgery, Sun Yat-sen University Cancer Center, Guangdong, 510060, China

    Feng-jiao Luo  (罗凤姣)

  5. The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410005, China

    Wen-tao Huang  (黄文韬)

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  1. Zong-li Liao  (廖宗力)
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Correspondence to Xiao-rong Chang  (常小荣).

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Author: Liao Zong-li, doctoral degree candidate

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Liao, Zl., Zhu, Cz., Tan, J. et al. Effects of different transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids, HSL and HMG-CoA reductase in hyperlipidemia rabbits. J. Acupunct. Tuina. Sci. 18, 157–164 (2020). https://doi.org/10.1007/s11726-020-1174-z

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