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Electroacupuncture on serum interleukin level in rat models of cerebral ischemia-reperfusion injury

电针对脑缺血再灌注模型大鼠血清白介素的影响

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Abstract

Objective

To observe the effect of electroacupuncture (EA) on serum interleukin (IL)-6, IL-8 and IL-10 in rat models of cerebral ischemia-reperfusion, and to discover the mechanism of EA in preventing and treating cerebral ischemia.

Methods

Male Sprague Dawley (SD) rats were randomized into a sham-operation (SO) group, a model control (MC) group, and an EA group, which were sub-grouped into a 6-hour group and a 24-hour group. In the SO group, rats only received vessel separation with filament placed inside without any treatment. In the MC and EA groups, the focal cerebral ischemia-reperfusion model was induced by using modified Longa method with intraluminal filament. The MC group didn’t receive any treatment; the EA group received EA at Baihui (GV 20) and Dazhui (GV 14) with sparse-dense wave for 30 min. The levels of serum IL-6, IL-8 and IL-10 were detected by using Elisa test.

Results

Six hours after ischemia-reperfusion injury, the levels of serum IL-6, IL-8 and IL-10 in the MC group were significantly higher than those in the SO group (P<0.01, P<0.05, P<0.05); the level of serum IL-8 in the EA group was significantly lower than that in the MC group (P<0.05), while there were no significant differences in comparing IL-6 and IL-10 between the EA group and the MC group. Twenty-four hours after ischemia-reperfusion injury, the levels of serum IL-6 and IL-8 in the EA group were significantly lower than those in the MC group (both P<0.05), while there were no significant differences in comparing the level of IL-10 among the three groups.

Conclusion

Early intervention by EA can regulate the levels of serum IL-6 and IL-8 in cerebral ischemic injury.

摘要

目的

观察电针对脑缺血再灌注模型大鼠血清白介素-6(interleukin-6, IL-6)、 IL-8和IL-10的影响, 探讨电针防治缺血性脑病的作用机理。

方法

雄性Sprague Dawley (SD)大鼠按随机数字表分为假手术组、 模型组和电针组, 以上三组又各分为6 h和24 h两个亚组。 假手术组仅予手术血管分离, 插入线栓, 不进行治疗。 模型组及电针组采用改良Longa线栓法复制大脑中动脉局灶性脑缺血再灌注模型。 模型组仅造模, 不作任何治疗; 电针组治疗取百会(GV 20)及大椎(GV 14), 采用电针疏密波刺激30 min。 应用Elisa法测定各组大鼠IL-6、 IL-8和L-10水平。

结果

脑缺血再灌注后6 h, 模型组血清IL-6、 IL-8及IL-10水平均较假手术组高(P<0.01, P<0.05, P<0.05); 电针组血清IL-8水平低于模型组(P<0.05), 血清IL-6及IL-10水平与模型组无统计学差异。 脑缺血再灌注后24 h, 电针组血清IL-6及IL-8水平低于模型组(均P<0.05), 血清IL-10水平三组比较均无统计学差异。

结论

电针早期介入脑缺血损伤可以调节血清IL-6、 IL-8水平。

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, the People’s Hospital of Jimo, Qingdao, Shandong, 266200, China

    Ping Wang  (王平)

  2. The Second Medical College of Nanjing University of Chinese Medicine, Jiangsu, 210046, China

    Yan-yun Mu  (穆艳云), Jie Cheng  (程洁), Jie Shen  (沈洁), Mei-hong Shen  (沈梅红), Xia Chen  (陈霞), Qian Li  (李茜), Yong Sun  (孙永) & Mei-rong Gong  (龚美蓉)

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Correspondence to Yan-yun Mu  (穆艳云).

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Wang, P., Mu, Yy., Cheng, J. et al. Electroacupuncture on serum interleukin level in rat models of cerebral ischemia-reperfusion injury. J. Acupunct. Tuina. Sci. 13, 9–14 (2015). https://doi.org/10.1007/s11726-015-0815-0

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  • DOI: https://doi.org/10.1007/s11726-015-0815-0

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