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Development of new ionic gelation strategy: Towards the preparation of new monodisperse and stable hyaluronic acid/β-cyclodextrin-grafted chitosan nanoparticles as drug delivery carriers for doxorubicin

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Abstract

In the present study, β-cyclodextrin-grafted chitosan nanoparticles (β-CD-g-CS NPs) were prepared using a new ionic gelation strategy involving a synergistic effect of NaCl (150 mmol/L), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 10 mmol/L), and water bath sonication. This new strategy afforded smaller and more monodisperse β-CD-g-CS NPs vs. the classical ionic gelation method. New HA/β-CD-g-CS NPs were also prepared using the above-mentioned strategy by adding hyaluronic acid (HA) to the β-CD-g-CS copolymer at different weight ratios until the ZP values conversion. The best result was obtained with the weight ratio of w(HA):w(β-CD-g-CS) = 2:1 and furnished new spherical and smooth HA/β-CD-g-CS NPs. Furthermore, the stability of β- CD-g-CS NPs and HA/β-CD-g-CS NPs at 4°C in physiological medium (pH 7.4) was compared for 3 weeks period and showed that HA/β-CD-g-CS NPs were more stable all maintaining their monodispersity and high negative ZP values compared to β-CD-g-CS NPs. Finally, preliminary study of HA/β-CD-g-CS NPs as carrier for the controlled release of the anticancer drug doxorubicin was investigated. These new HA/β-CD-g-CS NPs can potentially be used as drug delivery and targeting systems for cancer treatment.

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Acknowledgements

A.B.M. was supported by the Algerian Ministry of Higher Education and Scientific Research scholarship (No: 115/PNE: 2016/2017). The authors acknowledge the Dr. Benoît Frisch and Maria-Vittoria Spanedda (« Laboratoire de Conception et Application de Molécules Bioactives » UMR7199, Équipe de biovectorologie, Strasbourg) for nanoparticles characterizations.

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Authors and Affiliations

  1. Laboratoire de Chimie Physique Macromoléculaire (LCPM), Université de Lorraine-CNRS, UMR 7375, 1 Rue Grandville, BP 20451, 54001, Nancy Cedex, France

    Amina Ben Mihoub, Régis Vanderesse & Samir Acherar

  2. Laboratory of Physical Chemistry of Materials (LPCM), Faculty of Sciences, (UATL), BP 37G, Laghouat, 03000, Algeria

    Amina Ben Mihoub & Boubakeur Saidat

  3. Laboratory of Biomaterials & Transport Phenomena (LBTP), Quartier Ain D’Heb, 26000, Medea, Algeria

    Youssef Bal

  4. Department of Chemistry, Faculty of Sciences, University Saéd Dahleb of Blida (USDB), route de Soumaé, 09000, Blida, Algeria

    Youssef Bal

  5. Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine-CNRS, UMR 7274, 1 Rue Grandville, BP 20451, 54001, Nancy Cedex, France

    Céline Frochot

Authors
  1. Amina Ben Mihoub
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  2. Boubakeur Saidat
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  3. Youssef Bal
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  4. Céline Frochot
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  5. Régis Vanderesse
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  6. Samir Acherar
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Correspondence to Samir Acherar.

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Mihoub, A.B., Saidat, B., Bal, Y. et al. Development of new ionic gelation strategy: Towards the preparation of new monodisperse and stable hyaluronic acid/β-cyclodextrin-grafted chitosan nanoparticles as drug delivery carriers for doxorubicin. Front. Mater. Sci. 12, 83–94 (2018). https://doi.org/10.1007/s11706-018-0407-2

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  • DOI: https://doi.org/10.1007/s11706-018-0407-2

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