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Electrochemical and spectroelectrochemical characterization of different mesoporous TiO2 film electrodes for the immobilization of Cytochrome c

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Abstract

In this work three different mesoporous TiO2 film electrodes were prepared and used for the immobilization of Cytochrome c (Cyt-c). Films prepared via a standard sol-gel route (SG-films) were compared with commercially available benchmark nanotitania materials, namely P25 Degussa (P25-films) and Dyesol nanopaste (Dyesol films). Their properties, film deposition characteristics and their abilities to adsorb protein molecules in a stable and functional way were examined. We investigated whether it is possible, rather than preparing TiO2 films using multistep, lengthy and not always reproducible sol-gel procedures, to use commercially available nanotitania materials and produce reproducible films faster that exhibit all the properties that make TiO2 films ideal for protein immobilization. Although these materials are formulated primarily for dye-sensitized solar cell applications, in this study we found out that protein immobilization is facile and remarkably stable on all of them. We also investigated their electrochemical properties by using cyclic voltammetry and spectroelectrochemistry and found out that not only direct reduction of Fe(III)-heme to Fe(II)-heme of immobilized Cyt-c was possible on all films but that the adsorbed protein remained electroactive.

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References

  1. Solanki P R, Kaushik A, Agrawal V V, et al. Nanostructured metal oxide-based biosensors. NPG Asia Materials, 2011, 3(1): 17–24

    Article  Google Scholar 

  2. Bisquert J, Fabregat-Santiago F, Mora-Sero I, et al. A review of recent results on electrochemical determination of the density of electronic states of nanostructured metal-oxide semiconductors and organic hole conductors. Inorganica Chimica Acta, 2008, 361 (3): 684–698

    Article  Google Scholar 

  3. Obare S O, Ito T, Balfour M H, et al. Ferrous hemin oxidation by organic halides at nanocrystalline TiO2 interfaces. Nano Letters, 2003, 3(8): 1151–1153

    Article  Google Scholar 

  4. Topoglidis E, Campbell C J, Cass A E G, et al. Factors that affect protein adsorption on nanostructured titania films. A novel spectroelectrochemical application to sensing. Langmuir, 2001, 17(25): 7899–7906

    Google Scholar 

  5. Topoglidis E, Cass A E G, O’Regan B, et al. Immobilization and bioelectrochemistry of proteins on nanoporous TiO2 and ZnO films. Journal of Electroanalytical Chemistry, 2001, 517(1–2): 20–27

    Article  Google Scholar 

  6. Topoglidis E, Astuti Y, Duriaux F, et al. Direct electrochemistry and nitric oxide interaction of heme proteins adsorbed on nanocrystalline tin oxide electrodes. Langmuir, 2003, 19(17): 6894–6900

    Article  Google Scholar 

  7. Li Q, Luo G, Feng J. Direct electron transfer for heme proteins assembled on nanocrystalline TiO2 film. Electroanalysis, 2001, 13 (5): 359–363

    Article  Google Scholar 

  8. Jiang G, Tang H, Zhu L, et al. Improving electrochemical properties of liquid phase deposited TiO2 thin films by doping sodium dodecylsulfonate and its application as bioelectrocatalytic sensor for hydrogen peroxide. Sensors and Actuators B: Chemical, 2009, 138(2): 607–612

    Article  Google Scholar 

  9. Renault C, Balland V, Martinez-Ferrero E, et al. Highly ordered transparent mesoporous TiO2 thin films: an attractive matrix for efficient immobilization and spectroelectrochemical characterization of cytochrome c. Chemical Communications, 2009, 48(48): 7494–7496

    Article  Google Scholar 

  10. Yang D H, Takahara N, Mizutani N, et al. Fabrication of TiO2 and cytochrome c alternate ultrathin films via a gas-phase surface sol–gel process. Chemistry Letters, 2006, 35(9): 990–991

    Article  Google Scholar 

  11. McKenzie K J, Marken F. Accumulation and reactivity of the redox protein cytochrome c in mesoporous films of phytate. Langmuir, 2003, 19(10): 4327–4331

    Article  Google Scholar 

  12. Kim H, Park S S, Seo J, et al. Stable protein device platform based on pyridine dicarboxylic acid-bound cubic-nanostructured mesoporous titania films. ACS Applied Materials & Interfaces, 2013, 5 (15): 6873–6878

    Article  Google Scholar 

  13. Yang D H, Shin M J, Choi S M, et al. Cytochrome c assembly on fullerene nanohybrid metal oxide ultrathin films. RSC Advances, 2016, 6(23): 19173–19181

    Article  Google Scholar 

  14. Liu L, Wang N, Cao X, et al. Direct electrochemistry of cytochrome c at a hierarchically nanostructured TiO2 quantum electrode. Nano Research, 2010, 3(5): 369–378

    Article  Google Scholar 

  15. McKenzie K J, Marken F, Opallo M. TiO2 phytate films as hosts and conduits for cytochrome c electrochemistry. Bioelectrochemistry, 2005, 66(1–2): 41–47

    Article  Google Scholar 

  16. Topoglidis E, Lutz T, Durrant J R, et al. Interfacial electron transfer on cytochrome-c sensitised conformally coated mesoporous TiO2 films. Bioelectrochemistry, 2008, 74(1): 142–148

    Article  Google Scholar 

  17. Rafiee-Pour H A, Hamadanian M, Koushali S K. Nanocrystalline TiO2 films containing sulfur and gold: Synthesis, characterization and application to immobilize and direct electrochemistry of cytochrome c. Applied Surface Science, 2016, 363: 604–612

    Article  Google Scholar 

  18. Qiu J, Zhang S, Zhao H. Recent applications of TiO2 nanomaterials in chemical sensing in aqueous media. Sensors and Actuators B: Chemical, 2011, 160(1): 875–890

    Article  Google Scholar 

  19. Wang R, Zhang J, Hu Y. Liquid phase deposition of hemoglobin/ SDS/TiO2 hybrid film preserving photoelectrochemical activity. Bioelectrochemistry, 2011, 81(1): 34–38

    Article  Google Scholar 

  20. Luo Y, Tian Y, Zhu A, et al. pH-dependent electrochemical behavior of proteins with different isoelectric points of the nanostructured TiO2 surface. Journal of Electroanalytical Chemistry, 2010, 642(2): 109–114

    Article  Google Scholar 

  21. Renault C, Nicole L, Sanchez C, et al. Unraveling the charge transfer/electron transport in mesoporous semiconductive TiO2 films by voltabsorptometry. Physical Chemistry Chemical Physics, 2015, 17(16): 10592–10607

    Article  Google Scholar 

  22. Kityakarn S, Pooarporn Y, Songsiriritthigul P, et al. (Photo) Electrochemical characterization of nanoporous TiO2 and Cedoped TiO2 sol–gel film electrodes. Electrochimica Acta, 2012, 83: 113–124

    Article  Google Scholar 

  23. Dai G, Zhao L, Wang S, et al. Double-layer composite film based on sponge-like TiO2 and P25 as photoelectrode for enhanced efficiency in dye-sensitized solar cells. Journal of Alloys and Compounds, 2012, 539: 264–270

    Article  Google Scholar 

  24. Lee M S, Cheon I C, Kim Y I. Photoelectrochemical studies on nanocrystalline TiO2 film electrodes. Bulletin of the Korean Chemical Society, 2003, 24(8): 1155–1162

    Article  Google Scholar 

  25. Moore G R, Pettigrew G W. Cytochrome c: Evolution, Structure, and Physicochemical Aspects. Berlin: Springer-Verlag, 1990

    Book  Google Scholar 

  26. Stellwagen E. Haem exposure as the determinate of oxidation–reduction potential of haem proteins. Nature, 1978, 275(5675): 73–74

    Article  Google Scholar 

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Acknowledgement

This work was partially supported by a small initiative grant from the University of Patras.

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Authors and Affiliations

  1. Department of Materials Science, University of Patras, Rion, 26504, Greece

    Stavros Katsiaounis, Christina Tiflidis, Christina Tsekoura & Emmanuel Topoglidis

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  1. Stavros Katsiaounis
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  2. Christina Tiflidis
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  3. Christina Tsekoura
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  4. Emmanuel Topoglidis
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Correspondence to Emmanuel Topoglidis.

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Katsiaounis, S., Tiflidis, C., Tsekoura, C. et al. Electrochemical and spectroelectrochemical characterization of different mesoporous TiO2 film electrodes for the immobilization of Cytochrome c. Front. Mater. Sci. 12, 64–73 (2018). https://doi.org/10.1007/s11706-018-0406-3

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  • DOI: https://doi.org/10.1007/s11706-018-0406-3

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