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Evaluation of hydrogels for soft tissue adhesives in vitro and in vivo analyses

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Abstract

In this study, natural materials (sodium alginate, dextran, gelatin and carboxymethyl chitosan) were modified to get aldehyde components and amino components. Upon mixing the two-component solutions together, four kinds of Schiff base hydrogels formed successfully within 5-300 s and could seal the wound tissue. The cytotoxicity tests of hydrogel extraction solution confirmed that the hydrogels are nontoxic materials. The adhesive ability was evaluated in vivo by measuring the adhesive strength after sealing the skin incisions on the back of rats. All the hydrogels showed higher adhesive strength than that of commercial fibrin glue and the blank control. The histological staining observation by hematoxylin and eosin staining (HE) and Masson’s trichrome staining (MTC) methods suggested that the hydrogels had good biocompatibility and biodegradation in vivo. They have only normal initial inflammation to skin tissue and could improve the formation of new collagen in the incision section. So, the prepared hydrogels were both safe and effective tissue adhesive, which had the great potentials to be used as skin tissue adhesive.

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Acknowledgements

This research was supported by the National Key Research Program of China (2016YFA0201702 of 2016YFA0201700), the National Natural Science Foundation of China (Grant No. 31470941), the Science and Technology Commission of Shanghai Municipality (Nos. 15JC1490100, 15441905100), Donghua University Graduate Student Innovation Fund Project (CUSF-DH-D-2015032), Yantai Double Hundred Talent Plan and “111 Project” Biomedical Textile Materials Science and Technology, China (No. B07024).

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Author notes

  1. L.Y. and W.F. contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, China

    Liu Yuan, Meifang Zhu & Xiumei Mo

  2. Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China

    Liu Yuan, Linyingjun Han & Xiumei Mo

  3. Department of Orthopedics, Zhongshan Hospital of Fudan University, 180 Fenglin Road, Shanghai, 200032, China

    Wenshuai Fan, Changan Guo & Zuoqin Yan

  4. Shandong International Biotechnology Park Development Co., Ltd., Yantai, 264003, China

    Xiumei Mo

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  1. Liu Yuan
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  3. Linyingjun Han
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  4. Changan Guo
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Correspondence to Xiumei Mo.

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Yuan, L., Fan, W., Han, L. et al. Evaluation of hydrogels for soft tissue adhesives in vitro and in vivo analyses. Front. Mater. Sci. 12, 95–104 (2018). https://doi.org/10.1007/s11706-018-0405-4

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  • DOI: https://doi.org/10.1007/s11706-018-0405-4

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