Si-doping bone composite based on protein template-mediated assembly for enhancing bone regeneration
Bio-inspired hybrid materials that contain organic and inorganic networks interpenetration at the molecular level have been a particular focus of interest on designing novel nanoscale composites. Here we firstly synthesized a series of hybrid bone composites, silicon-hydroxyapatites/silk fibroin/collagen, based on a specific molecular assembled strategy. Results of material characterization confirmed that silicate had been successfully doped into nano-hydroxyapatite lattice. In vitro evaluation at the cellular level clearly showed that these Si-doped composites were capable of promoting the adhesion and proliferation of rat mesenchymal stem cells (rMSCs), extremely enhancing osteoblastic differentiation of rMSCs compared with silicon-free composite. More interestingly, we found there was a critical point of silicon content in the composition on regulating multiple cell behaviors. In vivo animal evaluation further demonstrated that Si-doped composites enabled to significantly improve the repair of cranial bone defect. Consequently, our current work not only suggests fabricating a potential bone repair materials by integrating element-doping and molecular assembled strategy in one system, but also paves a new way for constructing multi-functional composite materials in the future.
Keywordssilicate-doped molecular assembly biomimetic bone bone regeneration osteoblastic differentiation
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This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 81461148032, 81471792, 31430029, 81601610 and 31670968), the HUST Key Innovation Team Foundation for Interdisciplinary Promotion (2016JCTD101), and the Research Fund for the Doctoral Program of Higher Education of China (20110142110034). We also thank the Analytical and Testing Center of HUST for XRD, FTIR and TEM testing.
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