Fabrication and characterization of Antheraea pernyi silk fibroin-blended P(LLA-CL) nanofibrous scaffolds for peripheral nerve tissue engineering
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Electrospun nanofibers have gained widespreading interest for tissue engineering application. In the present study, ApF/P(LLA-CL) nanofibrous scaffolds were fabricated via electrospinning. The feasibility of the material as tissue engineering nerve scaffold was investigated in vitro. The average diameter increased with decreasing the blend ratio of ApF to P(LLA-CL). Characterization of 13C NMR and FTIR clarified that there is no obvious chemical bond reaction between ApF and P(LLA-CL). The tensile strength and elongation at break increased with the content increase of P(LLA-CL). The surface hydrophilic property of nanofibrous scaffolds enhanced with the increased content of ApF. Cell viability studies with Schwann cells demonstrated that ApF/P(LLA-CL) blended nanofibrous scaffolds significantly promoted cell growth as compare to P(LLA-CL), especially when the weight ratio of ApF to P(LLA-CL) was 25:75. The present work provides a basis for further studies of this novel nanofibrous material (ApF/P(LLA-CL)) in peripheral nerve tissue repair or regeneration.
KeywordsApF/P(LLA-CL) electrospinning nanofibers scaffolds Schwann cells peripheral nerve tissue engineering
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This research was supported by the National Key Research Program of China (2016YFA0201702 of 2016YFA0201700), the National Natural Science Foundation of China (Grant Nos. 31470941 and 31271035), the Science and Technology Commission of Shanghai Municipality (Grant Nos. 15JC1490100 and 15441905100), the Ph. D. Programs Foundation of Ministry of Education of China (Grant No. 20130075110005), and the Yantai Double Hundred Talent Plan. The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for funding this research work through ISPP# 0049.
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