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Frontiers of Materials Science

, Volume 10, Issue 4, pp 346–357 | Cite as

The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

  • Xuran Guo
  • Kaile Zhang
  • Mohamed El-Aassar
  • Nanping Wang
  • Hany El-Hamshary
  • Mohamed El-Newehy
  • Qiang Fu
  • Xiumei Mo
Research Article

Abstract

Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. Collagen/poly(L-lactide-co-caprolactone) (Col/P(LLA-CL)) nanoyarn scaffolds were prepared by conjugated electrospinning and dynamic liquid electrospinning, respectively. In addition, co-axial electrospinning technique was combined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading Wnt signaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H&E staining, respectively. The release profiles of different scaffolds were determined using HPLC. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds prepared by dynamic liquid electrospinning (DLY). It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY could facilitate the infiltration of the fibroblasts and could be a promising scaffold for the treatment of urethra stricture while it may inhibit the collagen production.

Keywords

biomaterials nanoyarn electrospinning inhibitor 

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Supplementary material

11706_2016_359_MOESM1_ESM.pdf (50 kb)
Fig. S1 The H&E staining image of fibroblast seeded on nanofiber scaffold (NF).

References

  1. [1]
    van der Veer W M, Bloemen M C, Ulrich M M, et al. Potential cellular and molecular causes of hypertrophic scar formation. Burns, 2009, 35(1): 15–29CrossRefGoogle Scholar
  2. [2]
    Zhang H, Ran X, Hu C L, et al. Therapeutic effects of liposomeenveloped Ligusticum chuanxiong essential oil on hypertrophic scars in the rabbit ear model. PLoS One, 2012, 7(2): e31157CrossRefGoogle Scholar
  3. [3]
    Zhang K, Guo X, Zhao W, et al. Application of Wnt pathway inhibitor delivering scaffold for inhibiting fibrosis in urethra strictures: in vitro and in vivo study. International Journal of Molecular Sciences, 2015, 16(11): 27659–27676CrossRefGoogle Scholar
  4. [4]
    Qi L, Song W, Liu Z, et al. Wnt3a promotes the vasculogenic mimicry formation of colon cancer via Wnt/ß-catenin signaling. International Journal of Molecular Sciences, 2015, 16(8): 18564–18579CrossRefGoogle Scholar
  5. [5]
    Tan J, Tong B-D, Wu Y-J, et al. MicroRNA-29 mediates TGFß1- induced extracellular matrix synthesis by targeting wnt/ß-catenin pathway in human orbital fibroblasts. International Journal of Clinical and Experimental Pathology, 2014, 7(11): 7571–7577Google Scholar
  6. [6]
    Baarsma H A, Spanjer A I, Haitsma G, et al. Activation of WNT/ ß-catenin signaling in pulmonary fibroblasts by TGF-ß1 is increased in chronic obstructive pulmonary disease. PLoS One, 2011, 6(9): e25450CrossRefGoogle Scholar
  7. [7]
    Bergmann C, Akhmetshina A, Dees C, et al. Inhibition of glycogen synthase kinase 3ß induces dermal fibrosis by activation of the canonical Wnt pathway. Annals of the Rheumatic Diseases, 2011, 70(12): 2191–2198CrossRefGoogle Scholar
  8. [8]
    Conidi A, van den Berghe V, Huylebroeck D. Aptamers and their potential to selectively target aspects of EGF, Wnt/ß-catenin and TGFß-smad family signaling. International Journal of Molecular Sciences, 2013, 14(4): 6690–6719CrossRefGoogle Scholar
  9. [9]
    Park K, Lee K, Zhang B, et al. Identification of a novel inhibitor of the canonical Wnt pathway. Molecular and Cellular Biology, 2011, 31(14): 3038–3051CrossRefGoogle Scholar
  10. [10]
    Beyer C, Reichert H, Akan H, et al. Blockade of canonical Wnt signalling ameliorates experimental dermal fibrosis. Annals of the Rheumatic Diseases, 2013, 72(7): 1255–1258CrossRefGoogle Scholar
  11. [11]
    Chuang P Y, Menon M C, He J C. Molecular targets for treatment of kidney fibrosis. Journal of Molecular Medicine, 2013, 91(5): 549–559CrossRefGoogle Scholar
  12. [12]
    Hao S, He W, Li Y, et al. Targeted inhibition of ß-catenin/CBP signaling ameliorates renal interstitial fibrosis. Journal of the American Society of Nephrology, 2011, 22(9): 1642–1653CrossRefGoogle Scholar
  13. [13]
    Langer R, Vacanti J P. Tissue engineering. Science, 1993, 260(5110): 920–926CrossRefGoogle Scholar
  14. [14]
    Zhang Y, Venugopal J R, El-Turki A, et al. Electrospun biomimetic nanocomposite nanofibers of hydroxyapatite/chitosan for bone tissue engineering. Biomaterials, 2008, 29(32): 4314–4322CrossRefGoogle Scholar
  15. [15]
    Wu J, Liu S, He L, et al. Electrospun nanoyarn scaffold and its application in tissue engineering. Materials Letters, 2012, 89: 146–149CrossRefGoogle Scholar
  16. [16]
    Xu Y, Wu J, Wang H, et al. Fabrication of electrospun poly(Llactide-co-e-caprolactone)/collagen nanoyarn network as a novel, three-dimensional, macroporous, aligned scaffold for tendon tissue engineering. Tissue Engineering Part C: Methods, 2013, 19(12): 925–936CrossRefGoogle Scholar
  17. [17]
    Di Lullo G A, Sweeney S M, Korkko J, et al. Mapping the ligandbinding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. The Journal of Biological Chemistry, 2002, 277(6): 4223–4231CrossRefGoogle Scholar
  18. [18]
    Kim B S, Mooney D J. Development of biocompatible synthetic extracellular matrices for tissue engineering. Trends in Biotechnology, 1998, 16(5): 224–230CrossRefGoogle Scholar
  19. [19]
    Baker B M, Handorf A M, Ionescu L C, et al. New directions in nanofibrous scaffolds for soft tissue engineering and regeneration. Expert Review of Medical Devices, 2009, 6(5): 515–532CrossRefGoogle Scholar
  20. [20]
    Huang C, Chen R, Ke Q, et al. Electrospun collagen-chitosan-TPU nanofibrous scaffolds for tissue engineered tubular grafts. Colloids and Surfaces B: Biointerfaces, 2011, 82(2): 307–315CrossRefGoogle Scholar
  21. [21]
    Grover C N, Cameron R E, Best S M. Investigating the morphological, mechanical and degradation properties of scaffolds comprising collagen, gelatin and elastin for use in soft tissue engineering. Journal of the Mechanical Behavior of Biomedical Materials, 2012, 10: 62–74CrossRefGoogle Scholar
  22. [22]
    Ji W, Sun Y, Yang F, et al. Bioactive electrospun scaffolds delivering growth factors and genes for tissue engineering applications. Pharmaceutical Research, 2011, 28(6): 1259–1272CrossRefGoogle Scholar
  23. [23]
    Mirdailami O, Soleimani M, Dinarvand R, et al. Controlled release of rhEGF and rhbFGF from electrospun scaffolds for skin regeneration. Journal of Biomedical Materials Research Part A, 2015, 103(10): 3374–3385CrossRefGoogle Scholar
  24. [24]
    Baker B M, Handorf A M, Ionescu L C, et al. New directions in nanofibrous scaffolds for soft tissue engineering and regeneration. Expert Review of Medical Devices, 2009, 6(5): 515–532CrossRefGoogle Scholar
  25. [25]
    Lee H J, Lee S J, Uthaman S, et al. Biomedical applications of magnetically functionalized organic/inorganic hybrid nanofibers. International Journal of Molecular Sciences, 2015, 16(6): 13661–13677CrossRefGoogle Scholar
  26. [26]
    Li X Y, Li Y C, Yu D G, et al. Fast disintegrating quercetin-loaded drug delivery systems fabricated using coaxial electrospinning. International Journal of Molecular Sciences, 2013, 14(11): 21647–21659CrossRefGoogle Scholar
  27. [27]
    Stout D A. Recent advancements in carbon nanofiber and carbon nanotube applications in drug delivery and tissue engineering. Current Pharmaceutical Design, 2015, 21(15): 2037–2044CrossRefGoogle Scholar
  28. [28]
    Bonkat G, Braissant O, Rieken M, et al. Comparison of the rollplate and sonication techniques in the diagnosis of microbial ureteral stent colonisation: results of the first prospective randomised study. World Journal of Urology, 2013, 31(3): 579–584CrossRefGoogle Scholar
  29. [29]
    Lv Y. Nanofiber-based drug design, delivery and application. Current Pharmaceutical Design, 2015, 21(15): 1918–1919CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Xuran Guo
    • 1
  • Kaile Zhang
    • 2
  • Mohamed El-Aassar
    • 1
    • 3
  • Nanping Wang
    • 4
  • Hany El-Hamshary
    • 5
    • 6
  • Mohamed El-Newehy
    • 5
    • 6
  • Qiang Fu
    • 2
  • Xiumei Mo
    • 1
    • 7
  1. 1.State Key Lab for Modification of Chemical Fibers & Polymer Materials, College of Chemistry & Chemical Engineering and BiotechnologyDonghua UniversityShanghaiChina
  2. 2.Department of Urology, Affiliated Sixth People’s HospitalShanghai Jiaotong UniversityShanghaiChina
  3. 3.Polymer Materials Research Department, Advanced Technology and New Material InstituteCity of Scientific Research and Technological Applications (SRTA-City)New Borg El-Arab City, AlexandriaEgypt
  4. 4.Shanghai Aquatic Product Research InstituteShanghaiChina
  5. 5.Department of Chemistry, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  6. 6.Department of Chemistry, Faculty of ScienceTanta UniversityTantaEgypt
  7. 7.Shandong International Biotechnology Park Development Co., Ltd.ShanghaiChina

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