Frontiers of Materials Science

, Volume 10, Issue 4, pp 358–366 | Cite as

Sodium carboxymethylation-functionalized chitosan fibers for cutaneous wound healing application

  • Dong Yan
  • Zhong-Zheng Zhou
  • Chang-Qing Jiang
  • Xiao-Jie Cheng
  • Ming Kong
  • Ya Liu
  • Chao Feng
  • Xi-Guang Chen
Research Article


A water absorption biomaterial, sodium carboxymethylation-functionalized chitosan fibers (Na-NOCC fibers) were prepared, applied for cutaneous wound repair, and characterized by FTIR and NMR. The water absorption of Na-NOCC fibers increased significantly with substitution degree rising, from 3.2 to 6.8 g/g, and higher than that of chitosan fibers (2.2 g/g) confirmed by swelling behavior. In the antibacterial action, the high degree of substitution of Na-NOCC fibers exhibited stronger antibacterial activities against E. coli (from 66.54% up to 88.86%). The inhibition of Na-NOCC fibers against S. aureus were above 90%, and more effective than E. coli. The cytotoxicity assay demonstrated that Na-NOCC2 fibers were no obvious cytotoxicity to mouse fibroblasts. Wound healing test and histological examination showed that significantly advanced granulation tissue and capillary formation in the healing-impaired wounds treated with Na-NOCC fibers, as compared to those treated with gauze, which demonstrated that Na- NOCC fibers could promote skin repair and might have great application for wound healing.


sodium carboxymethylation-functionalized chitosan fiber water absorption wound healing wound dressing 


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  1. [1]
    Miraftab M, Masood R, Edward-Jones V. A new carbohydratebased wound dressing fibre with superior absorption and antimicrobial potency. Carbohydrate Polymers, 2014, 101: 1184–1190CrossRefGoogle Scholar
  2. [2]
    Babu R, Zhang J, Beckman E J, et al. Antimicrobial activities of silver used as a polymerization catalyst for a wound-healing matrix. Biomaterials, 2006, 27(24): 4304–4314CrossRefGoogle Scholar
  3. [3]
    Kanokpanont S, Damrongsakkul S, Ratanavaraporn J, et al. An innovative bi-layered wound dressing made of silk and gelatin for accelerated wound healing. International Journal of Pharmaceutics, 2012, 436(1–2): 141–153CrossRefGoogle Scholar
  4. [4]
    Lu S, Gao W, Gu H Y. Construction, application and biosafety of silver nanocrystalline chitosan wound dressing. Burns, 2008, 34(5): 623–628CrossRefGoogle Scholar
  5. [5]
    Sashiwa H, Aiba S I. Chemically modified chitin and chitosan as biomaterials. Progress in Polymer Science, 2004, 29(9): 887–908CrossRefGoogle Scholar
  6. [6]
    Muzzarelli R A A. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 2009, 76(2): 167–182CrossRefGoogle Scholar
  7. [7]
    Chang J, Liu W, Han B, et al. Investigation of the skin repair and healing mechanism of N-carboxymethyl chitosan in seconddegree burn wounds. Wound Repair and Regeneration, 2013, 21(1): 113–121CrossRefGoogle Scholar
  8. [8]
    Zhou Y, Yang H, Liu X, et al. Potential of quaternizationfunctionalized chitosan fiber for wound dressing. International Journal of Biological Macromolecules, 2013, 52: 327–332CrossRefGoogle Scholar
  9. [9]
    Anitha A, Divya Rani V V, Krishna R, et al. Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N,O-carboxymethyl chitosan nanoparticles. Carbohydrate Polymers, 2009, 78(4): 672–677CrossRefGoogle Scholar
  10. [10]
    Kim I Y, Seo S J, Moon H S, et al. Chitosan and its derivatives for tissue engineering applications. Biotechnology Advances, 2008, 26(1): 1–21CrossRefGoogle Scholar
  11. [11]
    Qin Y, Hu H, Luo A, et al. Effect of carboxymethylation on the absorption and chelating properties of chitosan fibers. Journal of Applied Polymer Science, 2006, 99(6): 3110–3115CrossRefGoogle Scholar
  12. [12]
    Upadhyaya L, Singh J, Agarwal V, et al. Biomedical applications of carboxymethyl chitosans. Carbohydrate Polymers, 2013, 91(1): 452–466CrossRefGoogle Scholar
  13. [13]
    Rinaudo M. Chitin and chitosan: Properties and applications. Progress in Polymer Science, 2006, 31(7): 603–632CrossRefGoogle Scholar
  14. [14]
    Kong X. Simultaneous determination of degree of deacetylation, degree of substitution and distribution fraction of–COONa in carboxymethyl chitosan by potentiometric titration. Carbohydrate Polymers, 2012, 88(1): 336–341CrossRefGoogle Scholar
  15. [15]
    Chen X G, Park H J. Chemical characteristics of O-carboxymethyl chitosans related to the preparation conditions. Carbohydrate Polymers, 2003, 53(4): 355–359CrossRefGoogle Scholar
  16. [16]
    Zhou Y, Shi L, Li F, et al. Preparation and characterization of carboxymethyl-functionalized chitosan fiber. Journal of Natural Fibers, 2015, 12(3): 211–221CrossRefGoogle Scholar
  17. [17]
    Muzzarelli R A A, Tanfani F, Emanuelli M, et al. N- (carboxymethylidene) chitosans and N-(carboxymethyl) chitosans: Novel chelating polyampholytes obtained from chitosan glyoxylate. Carbohydrate Research, 1982, 107(2): 199–214CrossRefGoogle Scholar
  18. [18]
    Lv J, Zhou Q, Liu G, et al. Preparation and properties of polyester fabrics grafted with O-carboxymethyl chitosan. Carbohydrate Polymers, 2014, 113: 344–352CrossRefGoogle Scholar
  19. [19]
    Sweeney I R, Miraftab M, Collyer G. Absorbent alginate fibres modified with hydrolysed chitosan for wound care dressings—II. Pilot scale development. Carbohydrate Polymers, 2014, 102: 920–927CrossRefGoogle Scholar
  20. [20]
    Zeng X, Sun Y X, Qu W, et al. Biotinylated transferrin/avidin/ biotinylated disulfide containing PEI bioconjugates mediated p53 gene delivery system for tumor targeted transfection. Biomaterials, 2010, 31(17): 4771–4780CrossRefGoogle Scholar
  21. [21]
    Li X, Kong X, Zhang Z, et al. Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for drug delivery application. International Journal of Biological Macromolecules, 2012, 50(5): 1299–1305CrossRefGoogle Scholar
  22. [22]
    Tao S, Gao S, Zhou Y, et al. Preparation of carboxymethyl chitosan sulfate for improved cell proliferation of skin fibroblasts. International Journal of Biological Macromolecules, 2013, 54: 160–165CrossRefGoogle Scholar
  23. [23]
    Zhou Z, Yan D, Cheng X, et al. Biomaterials based on N,N,Ntrimethyl chitosan fibers in wound dressing applications. International Journal of Biological Macromolecules, 2016, 89: 471–476CrossRefGoogle Scholar
  24. [24]
    Huang Y, Zhang L, Yang J, et al. Structure and properties of cellulose films reinforced by chitin whiskers. Macromolecular Materials and Engineering, 2013, 298(3): 303–310CrossRefGoogle Scholar
  25. [25]
    Ong S Y, Wu J, Moochhala SM, et al. Development of a chitosanbased wound dressing with improved hemostatic and antimicrobial properties. Biomaterials, 2008, 29(32): 4323–4332CrossRefGoogle Scholar
  26. [26]
    Dowling M B, Smith W, Balogh P, et al. Hydrophobicallymodified chitosan foam: description and hemostatic efficacy. The Journal of Surgical Research, 2015, 193(1): 316–323CrossRefGoogle Scholar
  27. [27]
    Upadhyaya L, Singh J, Agarwal V, et al. The implications of recent advances in carboxymethyl chitosan based targeted drug delivery and tissue engineering applications. Journal of Controlled Release, 2014, 186: 54–87CrossRefGoogle Scholar
  28. [28]
    Gong C, Wu Q, Wang Y, et al. A biodegradable hydrogel system containing curcumin encapsulated in micelles for cutaneous wound healing. Biomaterials, 2013, 34(27): 6377–6387CrossRefGoogle Scholar
  29. [29]
    Murakami K, Aoki H, Nakamura S, et al. Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials, 2010, 31(1): 83–90CrossRefGoogle Scholar
  30. [30]
    Brugnerotto J, Lizardi J, Goycoolea F M, et al. An infrared investigation in relation with chitin and chitosan characterization. Polymer, 2001, 42(8): 3569–3580CrossRefGoogle Scholar
  31. [31]
    Zhao Y W, Liu L, Han X, et al. Preparation of N, Ocarboxymethyl chitosan with different substitutional degree and its application for hemostasis. Advanced Materials Research, 2013, 798–799: 1061–1066CrossRefGoogle Scholar
  32. [32]
    Kubota N, Tatsumoto N, Sano T, et al. A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents. Carbohydrate Research, 2000, 324(4): 268–274CrossRefGoogle Scholar
  33. [33]
    Patrulea V, Applegate L A, Ostafe V, et al. Optimized synthesis of O-carboxymethyl-N,N,N-trimethyl chitosan. Carbohydrate Polymers, 2015, 122: 46–52CrossRefGoogle Scholar
  34. [34]
    Zheng M, Han B, Yang Y, et al. Synthesis, characterization and biological safety of O-carboxymethyl chitosan used to treat Sarcoma 180 tumor. Carbohydrate Polymers, 2011, 86(1): 231–238CrossRefGoogle Scholar
  35. [35]
    Lim S H, Hudson S M. Application of a fiber-reactive chitosan derivative to cotton fabric as an antimicrobial textile finish. Carbohydrate Polymers, 2004, 56(2): 227–234CrossRefGoogle Scholar
  36. [36]
    Lim S H, Hudson S M. Review of chitosan and its derivatives as antimicrobial agents and their uses as textile chemicals. Journal of Macromolecular Science Part C: Polymer Reviews, 2003, 43(2): 223–269CrossRefGoogle Scholar
  37. [37]
    Lim S H, Hudson S M. Synthesis and antimicrobial activity of a water-soluble chitosan derivative with a fiber-reactive group. Carbohydrate Research, 2004, 339(2): 313–319CrossRefGoogle Scholar
  38. [38]
    Raafat D, von Bargen K, Haas A, et al. Insights into the mode of action of chitosan as an antibacterial compound. Applied and Environmental Microbiology, 2008, 74(12): 3764–3773CrossRefGoogle Scholar
  39. [39]
    Kong M, Chen X G, Xing K, et al. Antimicrobial properties of chitosan and mode of action: a state of the art review. International Journal of Food Microbiology, 2010, 144(1): 51–63CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Dong Yan
    • 1
  • Zhong-Zheng Zhou
    • 1
  • Chang-Qing Jiang
    • 1
  • Xiao-Jie Cheng
    • 1
  • Ming Kong
    • 1
  • Ya Liu
    • 1
  • Chao Feng
    • 1
  • Xi-Guang Chen
    • 1
  1. 1.College of Marine Life ScienceOcean University of ChinaQingdaoChina

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