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In vitro effect of ethyl acetate, butanol and water extracts of Juniperus excelsa Bieb. on angiotensin-converting enzyme purified from human plasma

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Abstract

Angiotensin-converting enzyme (ACE) was purified from human plasma by affinity chromatography. Effect of ethyl acetate, butanol and water extracts of Juniperus excelsa Bieb. (J. excelsa) fruits on purified ACE activity was investigated. ACE was purified 3659-fold with a specific activity of 1350 EU/mg protein from human plasma. The purity and molecular weight of ACE were determined by SDS-PAGE, and two bands 60 kDa and 70 kDa are seen on the gel. Ethyl acetate extract of J. excelsa fruits showed an activation effect on human plasma ACE activity. Butanol and water extracts of J. excelsa fruits showed an inhibition effect on ACE activity. IC50 values for butanol and water extracts of J. excelsa fruits were calculated to be 2.858 mg/mL and 5.790 mg/mL, respectively. Lisinopril was used to be reference inhibitor. Type of inhibition for all inhibitors was designated as non-competitive. IC50 value and Ki constant for lisinopril were determined to be 0.781 nM and 0.662 nM, respectively. These results show that butanol and water extracts of J. excelsa plant may have an ACE inhibitor potency.

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Abbreviations

ACE:

Angiotensin-converting enzyme

J. excelsa :

Juniperus excelsa Bieb

RAAS:

Renin–angiotensin–aldosterone system

FAPGG:

N-[3-(2-Furyl) acryloyl]-l-phenylalanyl-glycyl-glycine

Na2B4O7.10H2O:

Sodium tetraborate

NHS:

N-hydroxysuccinimide

EDTA:

Ethylenediaminetetraacetic acid

TEMED:

N,N,N′,N′-Tetramethylethylenediamine

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

CHCl3 :

Chloroform

MeOH:

Methanol

CHX:

Chlorhexidine

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Authors and Affiliations

  1. Department of Nutrition and Dietetics, School of Health, Van Yüzüncü Yıl University, 65080, Van, Turkey

    Zehra Basi

  2. Department of Horticultural, Faculty of Agriculture, Van Yüzüncü Yıl University, Van, Turkey

    Nalan Turkoglu

  3. Department of Chemistry, Faculty of Science, Van Yüzüncü Yıl University, Van, Turkey

    Vedat Turkoglu & Fatih Karahan

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  1. Zehra Basi
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  2. Nalan Turkoglu
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Correspondence to Zehra Basi.

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Basi, Z., Turkoglu, N., Turkoglu, V. et al. In vitro effect of ethyl acetate, butanol and water extracts of Juniperus excelsa Bieb. on angiotensin-converting enzyme purified from human plasma. Chem. Pap. 73, 2525–2533 (2019). https://doi.org/10.1007/s11696-019-00806-w

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