Abstract
Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.
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This work was supported by the Specific University Research Grant (MUNI/A/0910/2017) provided by the Ministry of Education, Youth and Sports of the Czech Republic in the year 2017.
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Kostolanska, K., Novotna, L., Taborska, E. et al. Online solid-phase extraction liquid chromatography–mass spectrometry of hair cortisol using a surrogate analyte. Chem. Pap. 73, 151–158 (2019). https://doi.org/10.1007/s11696-018-0560-1
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DOI: https://doi.org/10.1007/s11696-018-0560-1