Chemical Papers

, Volume 72, Issue 11, pp 2899–2908 | Cite as

Efficient production of arachidonic acid of Mortierella sp. by solid-state fermentation using combinatorial medium with spent mushroom substrate

  • Sompot Antimanon
  • Warinthon Chamkhuy
  • Sarinya Sutthiwattanakul
  • Kobkul LaotengEmail author
Original Paper


Solid-state fermentation (SSF) is a bioconversion process for turning cheap agro-industrial materials to added-value products. For enrichment of agro-industrial materials with arachidonic acid (ARA; C20:4 n-6), SSF process of Mortierella sp. was developed by optimizing cultivation medium and parameters. The results showed that the fungal cultivation on the medium with optimal ratio of selected agricultural materials provided the fermented mass containing high ARA proportion of total fatty acid. Inclusion of the optimal medium with suitable amount of spent mushroom substrate, which was used as an internal support, significantly promoted the ARA production yield. Using the predicted quadratic model generated by Box–Behnken design, the maximal ARA production yield was achieved, thereby the fermentation parameter set for ARA production was experimentally validated using the developed medium formula. Of variables studied, the culture temperature and initial moisture content were important for the ARA production. The developed SSF process would provide a prospect for larger scale production of ARA by this fungal strain.


Arachidonic acid Agro-industrial materials Mortierella sp. Solid-state fermentation Spent mushroom substrate 



Arachidonic acid


Solid-state fermentation


Spent mushroom substrate


Total fatty acid


Polyunsaturated fatty acid


Fatty acid methyl ester



This work was funded by Cluster Program Management Office, National Science and Technology Development Agency, Thailand (grant number P-13-50368), and Research Initiative fund of Food Biotechnology Research Unit, BIOTEC (grant number P-17-51646).

Supplementary material

11696_2018_519_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)
11696_2018_519_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 15 kb)


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2018

Authors and Affiliations

  • Sompot Antimanon
    • 1
  • Warinthon Chamkhuy
    • 1
  • Sarinya Sutthiwattanakul
    • 1
  • Kobkul Laoteng
    • 1
    Email author
  1. 1.Bioprocess Technology Laboratory, Food Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Thailand Science ParkKhlong LuangThailand

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