Conductometric sensor with calixarene-based chemosensitive element for the arginine detection
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A possibility of the creation of conductometric chemosensor with calixarene-based sensitive element for arginine detection was evaluated. The surface of gold interdigitated electrodes of conductometric transducer was modified with calixarene. The optimal concentration of calixarene for preparation of chemosensitive element was determined to be 100 mg/ml. The basic analytical characteristics of the developed chemosensor were determined (sensitivity to arginine—37.5 μS/mM, limit of arginine detection 5 μM, linear range 0.005–150 μM, response time 150 s) and analyzed as regards its application for arginine determination. It was established for all types of developed sensors that they have good reproducibility of signals to arginine over one working day; the measurement error (RSD) did not exceed 5%. The selectivity of calixarene-based chemosensor to arginine was investigated as well as the selectivity of calixarene complexation with arginine compared with other amino acids.
KeywordsChemosensor Calixarene Arginine Conductometric transducer
The authors gratefully acknowledge the financial support of this study by the STCU (Project 6177) and Scientific and Technical Government Program “Smart sensor devices of a new generation based on modern materials and technologies” of National Academy of Sciences of Ukraine.
VIK and SVD proposed an idea of the development of conductometric chemosensors based on calixarene 3 for arginine determination. SOC suggested a new procedure of calixarene 3 synthesis. OIK and OSP took part in the chromatographic study of calixarene 3 complexation with arginine and other amino acids. OMS performed experiments with 31 P and 1H NMR. SVM and OVS performed the main experiments to development conductometric chemosensor with calixarene-based chemosensitive element for arginine detection. OOS and SOC prepared and arranged the article. VIK, SVD, SOC, OOS and SVM took part in the discussion about the possibility of developed chemosensor used for partially selective detection of arginine or as the element of multisensor for amino acid analysis. All authors read and approved the final manuscript.
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Conflict of interest
The authors declare that they have no competing interests.
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