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Optimization of the chemolithoautotrophic biofilm growth of Cupriavidus necator by means of electrochemical hydrogen synthesis

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Abstract

The chemolithoautotrophic biofilm growth of Cupriavidus necator on a platinum cathode is presented. Different current densities from 0 to − 2000 µA/cm2 were investigated. − 500 µA/cm2 was selected as the favourable current density for most optimal hydrogen production for biofilm growth. Lower and higher current densities cause a lower microbial surface coverage due to higher cell death because of nutrient deficiency or due to a fast hydrogen saturation of the medium solution where no impulse is present for the microorganisms to grow at the cathodic surface. The first microbial attachment of Cupriavidus necator and its surface coverage can lead to better understanding of biofilm development and control.

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Acknowledgements

Financial support by the Bundesministerium für Wirtschaft und Energie (BMWi) through the Federation for Industrial Research (AiF) (IGF project: 18150BG) is thankfully acknowledged. We are grateful to Cora Kroner and Dirk Holtmann from DECHEMA Forschungsinstitut for critical reading and a helpful cooperation.

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Correspondence to S. Bause.

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Bause, S., Decker, M., Neubauer, P. et al. Optimization of the chemolithoautotrophic biofilm growth of Cupriavidus necator by means of electrochemical hydrogen synthesis. Chem. Pap. 72, 1205–1211 (2018). https://doi.org/10.1007/s11696-018-0382-1

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  • DOI: https://doi.org/10.1007/s11696-018-0382-1

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