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High throughput method for multielemental analysis of horse hair by oaTOF-ICP-MS

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Abstract

An approach for high throughput reliable multielemental analysis of trace elements in a large number of horse hair samples was designed. Suitability of time-of-flight mass spectrometer (oaTOF-ICP-MS) for fast determination of unlimited numbers of isotopes in the low volume samples was demonstrated. Due to quasi-simultaneous capability of the oaTOF-ICP-MS the large number of highly valuated data with unaffected isotopic ratio in a very short time could be obtained. The choice of horse hair was obvious because of easy reachability and clear conception about horse nutritional habits and stabling. Such large data set with preserved isotopic ratios is ideal for statistical evaluation which could reveal some interesting interconnection between elemental composition of horse hair and the way of stabling, feeding, etc. Statistical treatment of the data is not a part of this study and will be presented later. We collected one hundred horse hair samples from horse stables through Czech Republic. Samples were washed by optimized washing process to eliminate exogenic contamination prior to digestion and following analysis. A determination of 36 elements (As, Au, B, Be, Cd, Ce, Co, Cr, Dy, Er, Eu, Ga, Gd, Ge, Ho, La, Li, Lu, Nd, Ni, Pb, Pd, Pr, Pt, Rb, Sb, Sc, Sm, Ta, Tb, Te, Tm, U, V, Y, Yb) in horse hair by oaTOF-ICP-MS was optimized. A throughput of 100 samples with unlimited numbers of isotopes per 6 h was achieved. Proposed very fast multielemental method preserves isotopic ratios, and therefore, is undoubtedly highly suitable for statistical studies. Detection limits of the proposed method ranged from 0.13 μg kg−1 (Eu, Gd, Tm) to 27.9 μg kg−1 (Au), except for Ni (48.5 μg kg−1) that is probably affected by contamination raised from nickel cones.

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References

  • Anielski P et al (2005) Detection of testosterone, nandrolone and precursors. Anal Bioanal Chem 383:903–908. doi:10.1007/s00216-005-0104-8

    Article  CAS  Google Scholar 

  • Armelin MJA, Ávila RL, Piasentin RM, Saiki M (2001) Application of neutron activation analysis to evaluate the health status of equines by means of Cu, Fe, Mn and Zn determinations in their hair. J Radioanal Nucl Chem 249:417–419. doi:10.1023/A:1013239224784

    Article  CAS  Google Scholar 

  • Asano K, Suzuki K, Chiba M, Sera K, Asano R, Sakai T (2002) Concentrations of toxic metals and essential minerals in the mane hair of healthy racing horses and their relation to age. J Vet Med Sci 64:607–610. doi:10.1292/jvms.64.607

    Article  CAS  Google Scholar 

  • Asano K, Suzuki K, Chiba M, Sera K, Matsumoto T, Asano R, Sakai T (2005a) Influence of the coat color on the trace elemental status measured by particle–inducted X-ray emission in horse hair. Biol Trace Elem Res 103:169–176. doi:10.1385/BTER:103:2:169

    Article  CAS  Google Scholar 

  • Asano K, Suzuki K, Chiba M, Sera K, Asano R, Sakai T (2005b) Twenty-eight element concentrations in mane hair samples of adult riding horses determined by particle-inducted X-ray emission. Biol Trace Elem Res 107:135–140. doi:10.1385/BTER:107:2:135

    Article  CAS  Google Scholar 

  • Asano K, Suzuki K, Chiba M, Sera K, Asano R, Sakai T (2006) Relationship between trace elements status in mane hair and fibrillation in horse. J Vet Med Sci 68:769–771. doi:10.1292/jvms.68.769

    Article  CAS  Google Scholar 

  • Baysal A, Akman S (2010) Determination of lead in hair and its segmental analysis by solid sampling electrothermal atomic absorption spectrometry. Spectrochim Acta 65:340–344. doi:10.1016/j.sab.2010.02.016

    Article  Google Scholar 

  • Borgese L, Zacco A, Bontempi E, Pellegatta M, Vigna L, Patrini L, Riboldi L, Rubino FM, Depero LE (2010) Use of total reflection X-ray fluorescence (TXRF) for the evaluation of heavy metal poisoning due to the improper use of a traditional ayurvedic drug. J Pharm Biomed Anal 52:787–790. doi:10.1016/j.jpba.2010.02.030

    Article  CAS  Google Scholar 

  • Chandola LC, Lordello AR (1983) Elemental analysis of horse hair by optical emission spectroscopy. Microchem J 28:87–90. doi:10.1016/0026-265X(83)90032-2

    Article  CAS  Google Scholar 

  • Chojnacka K, Górecka H, Chojnacki A, Górecki H (2005) Inter-element interactions in human hair. Environ Toxicol Pharmacol 20:368–374. doi:10.1016/j.etap.2005.03.004

    Article  CAS  Google Scholar 

  • Chojnacka K, Górecka H, Górecki H (2006) The influence of living habits and family relationships on element concentrations in human hair. Sci Total Environ 366:612–620. doi:10.1016/j.scitotenv.2005.12.005

    Article  CAS  Google Scholar 

  • Dunnett M (2005) Hair analysis for screening horses for exposure to dietary toxic residues. Pferdeheilkunde 21:457–467

    Article  Google Scholar 

  • Dunnett M, Lees P (2003) Trace element, toxin and drug elimination in hair with particular reference to the horse. Res Vet Sci 75:89–101. doi:10.1016/S0034-5288(03)00074-2

    Article  CAS  Google Scholar 

  • Esteban M, Castano A (2009) Non-invasive matrices in human biomonitoring: a review. Environ Int 35:438–449. doi:10.1016/j.envint.2008.09.003

    Article  CAS  Google Scholar 

  • Kazi TG, Jamali MK, Arain MB, Afridi HI, Jalbani N, Sarfaz RA, Ansari R (2009) Evaluation of an ultrasonic acid digestion procedure for total heavy metals determination in environmental and biological samples. J Hazard Mater 161:1391–1398. doi:10.1016/j.jhazmat.2008.04.103

    Article  CAS  Google Scholar 

  • Kolacz R, Bodak E, Dobrzanski Z, Patkowska-Sokola B (1999) Trace elements in the wool of Polish merino sheep grazed in polluted and unpolluted environment. J Anim Sci 44:509–514

    CAS  Google Scholar 

  • Madejón P, Domínguez MT, Murillo JM (2009) Evaluation of pastures for horses grazing on soils polluted by trace elements. Ecotoxicology 18:417–428. doi:10.1007/s10646-009-0296-3

    Article  Google Scholar 

  • Patkowska-Sokola B, Dobrzanski Z, Osman K, Bodkowski R, Zygadlik K (2009) The content of chosen chemical elements in wool of sheep of different origins and breeds. Arch Anim Breed 52:410–418

    CAS  Google Scholar 

  • Rao KS, Bajali T, Rao TP, Babu Y, Naidu GRK (2002) Determination of iron, cobalt, nickel, manganese, zinc, copper, cadmium and lead in human hair by inductively coupled plasma—atomic emission spectrometry. Spectrochim Acta 57:1333–1338. doi:10.1016/S0584-8547(02)00045-9

    Article  Google Scholar 

  • Ribeiro AS, Curtius AJ, Pozebon D (2000) Determination of As, Cd, Ni and Pb in human hair by electrothermal atomic absorption spectrometry after sample treatment with tetramethylammonium hydroxide. Microchem J 64:105–110. doi:10.1016/S0026-265X(99)00024-7

    Article  CAS  Google Scholar 

  • Rodrigues JL, Batista BL, Nunes JA, Passos CJS, Barbosa F (2008) A fast method for the determination of 16 elements in hair samples by inductively coupled plasma mass spectrometry (ICP-MS) with tetramethylammonium hydroxide solubilization at room temperature. J Anal At Spectrom 23:992–996. doi:10.1039/b800595h

    Article  CAS  Google Scholar 

  • Rodushkin I, Axelsson MD (2000) Application of double focusing sector field ICP-MS for multielemental characterization of human hair and nails. Part I. Analytical methodology. Sci Total Environ 250:83–100. doi:10.1016/S0048-9697(00)00369-7

    Article  CAS  Google Scholar 

  • Sobanska MA (2005) Wild boar hair (Sus scrofa) as a non-invasive indicator of mercury pollution. Sci Total Environ 339:81–88. doi:10.1016/j.scitotenv.2004.07.018

    Article  CAS  Google Scholar 

  • Steely S, Amarasiriwardena D, Jones J, Yanes J (2007) A rapid approach for assessment of arsenic exposure by elemental analysis of single strand of hair using laser ablation-inductively coupled plasma-mass spectrometry. Microchem J 86:235–240. doi:10.1016/j.microc.2007.03.009

    Article  CAS  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge the financial support of the research plan SGFChT05/2012. Providing samples from all subjects is gratefully appreciated.

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Authors and Affiliations

  1. Institute of Environmental and Chemical Engineering, University of Pardubice, Studentska 573, 532 10, Pardubice, Czech Republic

    Jakub Návesník, Anna Krejčová, Tomáš Černohorský & Iveta Petrovická

  2. Department of Biological and Biochemical Sciences, University of Pardubice, Studentska 573, 532 10, Pardubice, Czech Republic

    Aneta Pátková

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  1. Jakub Návesník
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  2. Anna Krejčová
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Correspondence to Jakub Návesník.

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Návesník, J., Krejčová, A., Černohorský, T. et al. High throughput method for multielemental analysis of horse hair by oaTOF-ICP-MS. Chem. Pap. 71, 991–998 (2017). https://doi.org/10.1007/s11696-016-0090-7

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  • DOI: https://doi.org/10.1007/s11696-016-0090-7

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