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Verification of the selectivity of a liquid chromatography method for determination of stilbenes and flavonols in red wines by mass spectrometry

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Abstract

Quantification of bioactive phenols, like stilbenes and flavonols (SaF), has been conducted to evaluate the nutraceutical potential of red wines. However, there is still a lack of full validated, fast and accessible liquid chromatography methods offering high selectivity and a simple procedure. We present here the use of a high-resolution mass spectrometer to evaluate the selectivity of a feasible and traditional liquid chromatography technique (HPLC–DAD) to analyze markers of aglycone SaF in red wines. The SaF compounds were tested: trans-resveratrol, trans-ε-viniferin, quercetin, myricetin, and kaempferol, as well as trans-cinnamic acid, one of their precursors. System suitability and validation tests were employed for the selected conditions (octylsilane column, methanol mobile phase, and gradient elution). The validation process ensured the HPLC–DAD method was selective, linear, sensitive, precise, accurate and robust. The method was then applied to red wine samples from the Campanha Gaúcha region, Southern Brazil. The real samples contained different SaF levels, showing that the method is applicable to routine use. Furthermore, this was the first SaF characterization of red wines from the Campanha Gaúcha, contributing to regional and product development.

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Abbreviations

t R :

Retention time (min)

V/V:

Volume/volume (%)

R s :

Resolution

TF:

Asymmetry

k :

Capacity factor

N :

Theoretical plates

r 2 :

Coefficient of determination

F calc :

Calculated Fisher value

F tab :

Fisher table value

n :

Number of repetitions

p :

Probability

m/z :

Mass/charge (Da)

λ max :

Maximum ultraviolet absorption wavelength (nm)

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Acknowledgements

Commercial samples of wines were kindly collected by Dr. Jorge Tonietto from Embrapa Uva e Vinho and his team (Brazilian Agricultural Research Corporation-Grape and Wine). The authors gratefully acknowledge the FINEP (Brazilian Innovation Agency) and PPGCF-UFRGS (Pharmaceutical Sciences Graduate Program-Federal University of Rio Grande do Sul) for the financial support. The authors also thank Stephani Amanda Lukasewicz Ferreira for reviewing the manuscript, Alessandra Russi for the illustrations and Dr. Fabio Rossi Cavalcanti for the statistical confirmations by OriginPro 8 software.

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Authors and Affiliations

  1. LACEM-Laboratório de Cromatografia e Espectrometria de Massas, Embrapa Uva e Vinho, Rua Livramento, 515, Bento Gonçalves, RS, 95700-000, Brazil

    Letícia F. da Silva, Celito C. Guerra & Alana Foresti

  2. Laboratório de Química Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre, RS, 90610-000, Brazil

    Letícia F. da Silva & Ana M. Bergold

  3. Tecnologia em Viticultura e Enologia, Instituto Federal do Rio Grande do Sul, Av. Osvaldo Aranha, 540, Bento Gonçalves, RS, 95700-000, Brazil

    Alana Foresti

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  1. Letícia F. da Silva
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  2. Celito C. Guerra
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  3. Alana Foresti
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Correspondence to Letícia F. da Silva.

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da Silva, L.F., Guerra, C.C., Foresti, A. et al. Verification of the selectivity of a liquid chromatography method for determination of stilbenes and flavonols in red wines by mass spectrometry. Chem. Pap. 71, 661–672 (2017). https://doi.org/10.1007/s11696-016-0066-7

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