Bioproduction of phenylacetic acid in airlift reactor by immobilized Gluconobacter oxydans
- 93 Downloads
Phenylacetic acid can be produced from 2-phenylethanol by selective oxidation. This process can be performed using Gluconobacter oxydans immobilized in LentiKats®, polyvinyl alcohol particles with lenticular shape. The biotransformation took place at non-growth conditions in an airlift bioreactor. The goal of this work was to examine the impact of the particles on the hydrodynamics and volumetric mass transfer coefficient for oxygen in the airlift reactor and to describe the activity of the immobilized biomass in such systems. For this purpose, the influence of aeration and 2-phenylethanol concentration on the activity of biomass was examined. Also, the effects of long-term storage of immobilized biomass, long duration of fermentation and repeated use of biomass on the rate of phenylacetic acid production were tested. The results were compared with the phenylacetic acid production by free cells of Gluconobacter oxydans. The results show that aeration of the airlift reactor has only a minor effect on the phenylacetic acid production for immobilized biomass but a significant one for free cells; however, the impact of 2-phenylethanol concentration on the production was notable for both forms of biocatalyst depending on the biomass concentration. Production ability of free cells was ten times higher than that of immobilized cells, but the production time was only a quarter of that of immobilized cells.
KeywordsPhenylacetic acid Gluconobacter oxydans Immobilization Airlift reactor Biotransformation
This work was supported by the Slovak Research and Development Agency under the contract No. APVV-0656-11 and by the grant VEGA 1/0687/16.
- Bertóková A, Vikartovská A, Bučko M, Gemeiner P, Tkáč J, Chorvát D, Štefuca V, Neděla V (2015) Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules. Biocatal Biotransform 33:111–120. doi: 10.3109/10242422.2015.1053470 CrossRefGoogle Scholar
- Chisti Y (1989) Airlift bioreactors. Elsevier Science Publishers, LondonGoogle Scholar
- Gandolfi R, Cavenago K, Gualandris R, Sinisterra Gago JV, Molinari F (2004) Production of 2-phenylacetic acid and phenylacetaldehyde by oxidation of 2-phenylethanol with free immobilized cells of Acetobacter aceti. Process Biochem 39:749–753. doi: 10.1016/S0032-9592(03)00185-7 CrossRefGoogle Scholar
- Trogl J, Bouskova A, Mrakota J, Pilarova V, Krudencova J, Mechurova J, Krizenecka S, Stloukal R (2011) Removal of nitrates from simulated ion-exchange brines with Paracoccus denitrificans encapsulated in Lentikats biocatalyst. Desalination 275:82–86. doi: 10.1016/j.desal.2011.02.033 CrossRefGoogle Scholar
- Vorlop, K. D. & Jekel, M. (2000). Process for preparing a biocatalyst with a polyvinyl alcohol gel and biocatalyst produced by this process. German patent DE 198 27 552Google Scholar
- Wang CT, Sun BG, Cao YP, Wang J, Zhang H (2008) Biosynthesis of natural 2-phenylethanol by yeast cells. Modern Chem Ind 28:38–43Google Scholar
- Wenner W (1952) Phenylacetamide. Org Synth 4:760Google Scholar