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Bioproduction of phenylacetic acid in airlift reactor by immobilized Gluconobacter oxydans

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Abstract

Phenylacetic acid can be produced from 2-phenylethanol by selective oxidation. This process can be performed using Gluconobacter oxydans immobilized in LentiKats®, polyvinyl alcohol particles with lenticular shape. The biotransformation took place at non-growth conditions in an airlift bioreactor. The goal of this work was to examine the impact of the particles on the hydrodynamics and volumetric mass transfer coefficient for oxygen in the airlift reactor and to describe the activity of the immobilized biomass in such systems. For this purpose, the influence of aeration and 2-phenylethanol concentration on the activity of biomass was examined. Also, the effects of long-term storage of immobilized biomass, long duration of fermentation and repeated use of biomass on the rate of phenylacetic acid production were tested. The results were compared with the phenylacetic acid production by free cells of Gluconobacter oxydans. The results show that aeration of the airlift reactor has only a minor effect on the phenylacetic acid production for immobilized biomass but a significant one for free cells; however, the impact of 2-phenylethanol concentration on the production was notable for both forms of biocatalyst depending on the biomass concentration. Production ability of free cells was ten times higher than that of immobilized cells, but the production time was only a quarter of that of immobilized cells.

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Acknowledgements

This work was supported by the Slovak Research and Development Agency under the contract No. APVV-0656-11 and by the grant VEGA 1/0687/16.

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Authors and Affiliations

  1. Faculty of Chemical and Food Technology, Institute of Chemical and Environmental Engineering, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Mário Mihaľ, Ivan Červeňanský & Jozef Markoš

  2. Faculty of Chemical and Food Technology, Institute of Biotechnology and Food Science, Slovak University of Technology, Radlinského 9, 812 37, Bratislava, Slovakia

    Martin Rebroš

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  1. Mário Mihaľ
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  2. Ivan Červeňanský
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  3. Jozef Markoš
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  4. Martin Rebroš
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Correspondence to Jozef Markoš.

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Mihaľ, M., Červeňanský, I., Markoš, J. et al. Bioproduction of phenylacetic acid in airlift reactor by immobilized Gluconobacter oxydans . Chem. Pap. 71, 103–118 (2017). https://doi.org/10.1007/s11696-016-0062-y

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