Response surface optimization of cholesterol extraction from lanolin alcohol by selective solvent crystallization
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A process to extract cholesterol from lanolin alcohol by selective solvent crystallization and recrystallization was developed. The crude lanosterol crystallization process was performed in methanol-acetone (3:1, v/v) as solvent at 18 mL g−1 liquid–solid ratio and 20.0 °C for 6 h. To be optimized by response surface methodology (RSM) coupled with central composite design, the process of cholesterol crystallization was carried out in acetic acid as solvent at 4.18 mL g−1 liquid–solid ratio and 26.1 °C for 6 h. Under the optimized conditions, cholesterol purity was substantially improved increasing from 17.5 to 76.2%, while the purity and recovery efficiency of cholesterol increased to 94.7 and 88.0% after recrystallization, respectively.
KeywordsSelective solvent crystallization Response surface methodology Cholesterol Crude lanosterol Extraction
This work was financially supported by the National Natural Science Foundation of China (Grant No. 21376166).
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