Chemical Papers

, Volume 71, Issue 1, pp 71–79 | Cite as

Response surface optimization of cholesterol extraction from lanolin alcohol by selective solvent crystallization

  • Hui Ding
  • Dan Zhao
  • Yujie GaoEmail author
Original Paper


A process to extract cholesterol from lanolin alcohol by selective solvent crystallization and recrystallization was developed. The crude lanosterol crystallization process was performed in methanol-acetone (3:1, v/v) as solvent at 18 mL g−1 liquid–solid ratio and 20.0 °C for 6 h. To be optimized by response surface methodology (RSM) coupled with central composite design, the process of cholesterol crystallization was carried out in acetic acid as solvent at 4.18 mL g−1 liquid–solid ratio and 26.1 °C for 6 h. Under the optimized conditions, cholesterol purity was substantially improved increasing from 17.5 to 76.2%, while the purity and recovery efficiency of cholesterol increased to 94.7 and 88.0% after recrystallization, respectively.


Selective solvent crystallization Response surface methodology Cholesterol Crude lanosterol Extraction 



This work was financially supported by the National Natural Science Foundation of China (Grant No. 21376166).


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2016

Authors and Affiliations

  1. 1.School of Environmental Science and EngineeringTianjin UniversityTianjinChina
  2. 2.Tianjin Academy of Environmental SciencesTianjinChina
  3. 3.Tianjin United Environmental Engineering Design Company LimitedTianjinChina

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