This study investigated the effects of high hydrostatic pressure (HHP) (100, 250, and 500 MPa for 5 min) on components and functions of thylakoid membrane extracted from spinach subjected to 26 days of storage. We aimed to better understand the changes of chlorophylls and chlorophyll–protein complexes under HHP conditions. HHP treatments maintained higher (p < 0.05) chlorophylls and soluble protein contents than thermal treatment in the whole storage duration. Protein peptide components, including PsaA, PsaB, CP43, CP47, LHCII, CP26, and CP24 were more stable in HHP-treated samples than those of thermally treated ones. Moreover, chlorophylls emission and excitation fluorescence spectra capacities of thylakoid membrane were better maintained in HHP-treated samples than thermally treated ones, indicating higher light-harvesting and excitation efficiencies of thylakoid membrane during storage. Hence, HHP treatments, especially for 100 and 250 MPa, are beneficial for retention of chlorophylls and chlorophyll–protein complexes by stabilizing components and functions of thylakoid membrane during storage.
High hydrostatic pressure Chlorophylls Chlorophyll–protein complexes Thylakoid membrane Storage
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This work was supported by National Natural Science Foundation of China (No. 31601525), National Natural Science Foundation of China (No. 31501543) and Scientific Research Fund of Hunan Provincial Education Department (16B123).