Table 4 Test program and results of three-factor quadratic regression orthogonal design

From: Selection of culture conditions for callus induction and proliferation by somatic embryogenesis of Pinus koraiensis

Test no. Z0 Z1 Z2 Z3 Z1Z2 Z1Z3 Z2Z3 Z1 Z2 Z3 Mean appreciation percentage
1 1 1 1 1 1 1 1 0.406 0.406 0.406 111.81 ± 9.47
2 1 1 1 − 1 1 − 1 − 1 0.406 0.406 0.406 222.71 ± 23.73
3 1 1 − 1 1 − 1 1 − 1 0.406 0.406 0.406 144.85 ± 28.27
4 1 1 − 1 − 1 − 1 − 1 1 0.406 0.406 0.406 129.11 ± 20.12
5 1 − 1 1 1 − 1 − 1 1 0.406 0.406 0.406 192.50 ± 7.77
6 1 − 1 1 − 1 − 1 1 − 1 0.406 0.406 0.406 201.43 ± 12.87
7 1 − 1 − 1 1 1 − 1 − 1 0.406 0.406 0.406 200.80 ± 22.27
8 1 − 1 − 1 − 1 1 1 1 0.406 0.406 0.406 170.00 ± 5.40
9 1 1.682 0 0 0 0 0 2.234 − 0.594 − 0.594 93.66 ± 16.31
10 1 − 1.682 0 0 0 0 0 2.234 − 0.594 − 0.594 137.50 ± 1.44
11 1 0 1.682 0 0 0 0 − 0.594 2.234 − 0.594 165.00 ± 6.12
12 1 0 − 1.682 0 0 0 0 − 0.594 2.234 − 0.594 153.33 ± 29.02
13 1 0 0 1.682 0 0 0 − 0.594 − 0.594 2.234 127.50 ± 5.20
14 1 0 0 − 1.682 0 0 0 − 0.594 − 0.594 2.234 237.50 ± 12.99
15 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 218.33 ± 56.74
16 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 301.67 ± 8.33
17 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 221.76 ± 1.67
18 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 211.67 ± 52.39
19 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 213.75 ± 18.64
20 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 258.33 ± 4.41
21 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 236.67 ± 20.48
22 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 245.00 ± 7.64
23 1 0 0 0 0 0 0 − 0.594 − 0.594 − 0.594 208.33 ± 19.65
  1. Zj represents the no normative variable of its corresponding experimental factor Xj