Gene cloning and expression analysis of limonene synthase in Syringa oblata and S. oblata var. alba

  • Zifei Yan
  • Ying Qin
  • Jian Zheng
  • Pingsheng Leng
  • Zenghui Hu
Original Paper
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Abstract

Syringa species are important ornamentals with strong floral scent, of which monoterpenes are the main component. In this study, a new monoterpene synthase gene, named SoLIM, was collected from the flowers of Syringa oblata and S. oblata var. alba using a homologous cloning method. The full-length cDNA of SoLIM was 1746 bp and encoded 581 amino acids. Sequence analysis showed that SoLIM contained the DDxxD and RRx8W motifs, which are two typical conserved monoterpene synthase motifs, and was thus classified as belonging to the Tpsb subfamily. Using quantitative reverse-transcription PCR, SoLIM was significantly expressed in the petals and pistils of S. oblata and S. oblata var. alba, respectively. SoLIM expression peaked earlier than the D-limonene emissions in the diurnal experiments, but occurred later when D-limonene had peaked during the flowering phase, indicating that differences in SoLIM gene expression and D-limonene emissions existed. The synthesis of floral scent is thus associated with diverse regulatory mechanisms that require further investigation.

Keywords

Floral scent Syringa oblata Syringa oblata var. alba Limonene synthase gene 

Notes

Acknowledgements

We would like to thank LetPub (www.letpub.com) for providing linguistic assistance during the preparation of this manuscript.

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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Zifei Yan
    • 1
  • Ying Qin
    • 1
  • Jian Zheng
    • 1
    • 2
  • Pingsheng Leng
    • 1
    • 2
  • Zenghui Hu
    • 1
    • 2
  1. 1.College of Landscape ArchitectureBeijing University of AgricultureBeijingPeople’s Republic of China
  2. 2.Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit TreesBeijingPeople’s Republic of China

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