Molecular cloning and characterization of a lipid transfer protein gene (PsLTP1) from Pinus sylvestris (L.)
- 21 Downloads
Plant nonspecific lipid transfer proteins (nsLTPs) are widely distributed through plant kingdom and are characterized by the presence of a central hydrophobic cavity, suitable for binding various hydrophobic molecules. Despite extensive research on nsLTP in different plant species, mostly angiosperm, and the great diversity of physiological processes in which they seem to be involved, their exact functions still remain unclear. Also, very limited experimental data are available on nsLTP in gymnosperm. In this study, we report for the first time on the molecular cloning of nsLTP, from Pinus sylvestris L. (PsLTP1, GenBank accession JN980402.1) and the expression pattern of PsLTP1 during ontogenesis and in response to environmental stress conditions. Total RNA from roots of 7-day old pine seedlings was used to isolate the cDNA clone, corresponding to Scots pine lipid transfer protein. The open reading frame of PsLTP1 consists of 372 bp encoding a protein of 123 amino acids. Amino acid sequence alignment revealed that mature PsLTP1 shares high level of similarity with nsLTP from other conifers and with well-studied nsLTPs from angiosperms. The PsLTP1 contains a 27-amino-acid N-terminal signal sequence and presents all the features of a plant nsLTP. Amino acid comparison analysis and 3D structure prediction showed that PsLTP1 is a type 1 nsLTP. The results of the expression analysis of Scots pine PsLTP1 gene revealed that its transcripts accumulate in actively growing tissues. Furthermore, transcription of PsLTP1 was upregulated in response to cold and salt treatments, and downregulated during acidic, osmotic and water stresses.
KeywordsScots pine Nonspecific lipid transfer protein (nsLTP) Molecular cloning Expression Abiotic stresses
- Carvalho AO, Souza-Filho GA, Ferreira BS, Branco AT, Araújo IS, Fernandes KV, Retamal CA, Gomes VM (2006) Cloning and characterization of a cowpea seed lipid transfer protein cDNA: expression analysis during seed development and under fungal and cold stresses in seedlings’ tissues. Plant Physiol Biochem 44:732–742CrossRefPubMedGoogle Scholar
- Hensiruk SA (2002) Forests of Ukraine. Shevchenko Scientific Society, Ukrainian State university of Forestry and Wood Technology, LvivGoogle Scholar
- Scheurer S, Lauer I, Foetisch K, San Miguel Moncin M, Retzek M, Hartz C, Enrique E, Lidholm J, Cistero-Bahima A, Vieths S (2004) Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion. J Allergy Clin Immunol 114:900–907CrossRefPubMedGoogle Scholar