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Cloning of flavonoid 3′,5′-hydroxylase and 3′-hydroxylase homologs from black currant (Ribes nigrum) and their differential expression at various fruit maturation stages

  • Xing-guo Li
  • Tian-tian Yang
  • Ze-yuan Yu
  • Jun-wei Huo
  • Qin Dong
  • Ya-dong Duan
  • Guang Yang
Original Paper

Abstract

Flavonoid 3′-hydroxylase (F3′H) and 3′,5′-hydroxylase (F3′5′H) generate precursor molecules for the synthesis of cyanidin-based anthocyanins (red) and delphinidin-based anthocyanins (blue to purple) in Ribes nigrum L. (black currant). In this study, full-length 1780 and 1650-bp cDNA homologs of RnF3′5′H1 and RnF3′H1 from black currant were identified and cloned using a homologous cloning technique. Data revealed that Rn F3′5′H1 and RnF3′H1 are homologs that encode enzymes involved in anthocyanin synthesis from different plants, which phylogenetically cluster with the CYP75B and CYP75A families in the P450 superfamily, respectively. The enzymes encoded by these two genes also shared a high homology with flavonoid hydroxylases identified from other plants. Furthermore, RnF3′5′H1 and RnF3′H1 levels were upregulated during fruit maturation. RnF3′5′H1 levels were associated with both anthocyanin and soluble carbohydrate levels in blackcurrant, while RnF3′H1 expression did not have such an association. The structure and expression patterns of RnF3′5′H1 and RnF3′H1 in blackcurrant were also characterized. Further studies should aid understanding of anthocyanin biosynthesis in black currant to develop molecular approaches and manipulate anthocyanin production in blackcurrant.

Keywords

Anthocyanin Flavonoid Gene cloning Expression Blackcurrant 

Supplementary material

11676_2017_560_MOESM1_ESM.docx (281 kb)
Supplementary material 1 (DOCX 281 kb)

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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xing-guo Li
    • 1
  • Tian-tian Yang
    • 2
  • Ze-yuan Yu
    • 1
  • Jun-wei Huo
    • 1
  • Qin Dong
    • 1
  • Ya-dong Duan
    • 3
  • Guang Yang
    • 3
  1. 1.College of Horticulture & Landscape ArchitectureNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.College of Wildlife ResourcesNortheast Forestry UniversityHarbinPeople’s Republic of China
  3. 3.Berries Research InstituteHeilongjiang Academy of Agricultural SciencesSuilingPeople’s Republic of China

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