Journal of Forestry Research

, Volume 29, Issue 3, pp 631–638 | Cite as

Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination

  • Ling Yang
  • Dongyan Zhang
  • Hongnan Liu
  • Cheng Wei
  • Jianan Wang
  • Hailong Shen
Original Paper
First Online:
Received:
Accepted:
  • 44 Downloads

Abstract

The effects of an exogenous nitric oxide donor (sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode (PTIO) and carboxy-PTIO potassium salt (cPTIO) on the embryo germination of Sorbus pohuashanensis were studied in a petri dish test. SNP at 0.5–5 mmol L−1 increased germination percentage, mean time to germination, germination index and germination energy compared with the control to different degrees. Treatment with 2 mmol L−1 SNP improved germination most significantly; embryo germination percentage for mother tree 1 (91.11%) and mother tree 2 (64.44%) were much higher than the control. In addition, excessive SNP levels did not enhance embryo germination. Combined treatment with SNP and an NO scavenger delayed embryo germination. Treatment with cPTIO inhibited embryo germination; germination percentage was 42.22% and was lower than that of the control. These results show that low concentrations of exogenous NO can enhance the embryo germination of S. pohuashanensis, providing a simple, effective way for promoting germination of S. pohuashanensis.

Keywords

Mountain ash Embryo germination Nitric oxide Sodium nitroprusside Nitric oxide scavenger 
This is a preview of subscription content, log in to check access

Notes

Acknowledgements

We thank all the colleagues in our lab for constructive discussion and technical support.

References

  1. Cao BJ, Reith MEA (2002) Nitric oxide scavenger carboxy-PTIO potentiates the inhibition of dopamine uptake by nitric oxide donors. Eur J Pharmacol 448(1):27–30CrossRefPubMedGoogle Scholar
  2. Chang QS, Lx Zhang, Yang W, Ss Zhou, Huang QZ, Lü FJ, Huang Y, Ge SH, Zhang TM (2016) Effects of exogenous nitric oxide on antioxidant activity and photosynthetic characteristics of Prunella vulgaris seedlings under NaCl stress. Acta Pratacult Sinica 25(7):121–130Google Scholar
  3. Deng W, Qing XG, Yang Y (2010) Effects of applying organic fertilizer on rice lodging resistance and yield. J Agric Sci Technol 11(2):98–101Google Scholar
  4. Gniazdowska A, Dobrzyńska U, Babańczyk T, Bogatek R (2007) Breaking the apple embryo dormancy by nitric oxide involves the stimulation of ethylene production. Planta 225(4):1051–1057CrossRefPubMedGoogle Scholar
  5. Gniazdowska A, Krasuska U, Czajkowska K, Bogatek R (2010) Nitric oxide, hydrogen cyanide and ethylene are required in the control of germination and undisturbed development of young apple seedlings. Plant Growth Regul 61(1):75–84CrossRefGoogle Scholar
  6. Jia HF, Zhang HY (2014) Effects of exogenous nitric oxide on seed germination and seedling physiological characteristics of Isatis indigotica under NaCl stress. Chin Tradit Herb Drugs 45(1):118–124Google Scholar
  7. Krasuska U, Ciacka K, Bogatek R, Gniazdowska A (2014) Polyamines and nitric oxide link in regulation of dormancy removal and germination of apple (Malus domestica, Borkh.) embryos. J Plant Growth Regul 33(3):590–601CrossRefGoogle Scholar
  8. Lamotte O, Courtois C, Barnavon L, Pugin A, Wendehenne D (2005) Nitric oxide in plants: the biosynthesis and cell signalling properties of a fascinating molecule. Planta 221(1):1–4CrossRefPubMedGoogle Scholar
  9. Libourel IGL, Bethke PC, Michele RD, Jones RL (2006) Nitric oxide gas stimulates germination of dormant Arabidopsis seeds: use of a flow-through apparatus for delivery of nitric oxide. Planta 223(4):813–820CrossRefPubMedGoogle Scholar
  10. Ma Z, Marsolais F, Bykova NV, Igamberdiev AU (2016) Nitric oxide and reactive oxygen species mediate metabolic changes in barley seed embryo during Germination. Front Plant Sci 7(126):1–13PubMedCentralPubMedGoogle Scholar
  11. Petruzzelli L, Coraggio I, Leubner-Metzger G (2000) Ethylene promotes ethylene biosynthesis during pea seed germination by positive feedback regulation of 1-aminocyclo-propane-1-carboxylic acid oxidase. Planta 211(1):144–149CrossRefPubMedGoogle Scholar
  12. Sanz L, Albertos P, Mateos I, Sánchez-Vicente I, Lechón T, Fernández-Marcos M, Lorenzo O (2015) Nitric oxide (NO) and phytohormones crosstalk during early plant development. J Exp Bot 66(10):2857–2868CrossRefPubMedGoogle Scholar
  13. Shen H (2006) Influencing factors to seed dormancy and germination characteristics of Sorbus pohuashanensis. Scientia Silvae Sinicae 42(10):133–138Google Scholar
  14. Tang WQ, Zhang YX, Liu BH (2014) Grafting survival rate in different strains of Sorbus spp. Prot For Sci Technol 7:14–15Google Scholar
  15. Wang P, Zhu JK, Lang Z (2015) Nitric oxide suppresses the inhibitory effect of abscisic acid on seed germination by S-nitrosylation of SnRK2 proteins. Plant Signal Behav 10(6):1–3Google Scholar
  16. Xu JW, Shen HL, Zhang XL, Zhang P, Huang J (2010) Sorbus pohuashanensis seed dispersal and germination and their relationships with population natural regeneration. Chin J Appl Ecol 21(10):2536–2544Google Scholar
  17. Yang L (2013) Effects of exogenous nitric oxide on embryo germination and ROS accumulation in seedling growth initial stage of Sorbus pohuashanensis. Scientia Silvae Sinicae 49(6):60–67Google Scholar
  18. Yang L, Shen HL (2011) Effect of electrostatic field on seed germination and seedling growth of Sorbus pohuashanesis. J For Res 22(1):27–34CrossRefGoogle Scholar
  19. Yang L, Zhang P (2011) Experimental course of tree seeds. Northeast Forestry University Press, Harbin, pp 85–87Google Scholar
  20. Yang L, Liu CP, Shen HL (2008) Effect of cold stratification durations and germination temperatures on seed germination of Sorbus Pohuashanensis. Seed 27(10):20–22Google Scholar
  21. Yang L, Shen HL, Liu CP, Zhai XJ (2010) Somatic embryogenesis from mature zygotic embryo explants of Sorbus pohuashanensis Hedl. Bull Bot Res 30(2):174–179Google Scholar
  22. Yin H, Liu Q (2004) Advances in studies on molecular biology of seed dormancy and germination. Chin Bull Bot 21(2):156–163Google Scholar
  23. Zhang YY (2015) Study on the Technology of Microcutting and Rooting in Sorbus aucuparia L. Planting in Harbin. Journal of Anhui. Agric Sci 43(22):128–131Google Scholar

Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Ling Yang
    • 1
  • Dongyan Zhang
    • 1
  • Hongnan Liu
    • 1
  • Cheng Wei
    • 1
  • Jianan Wang
    • 1
  • Hailong Shen
    • 1
  1. 1.State Key Laboratory of Forest Genetics and Breeding, School of ForestryNortheast Forestry UniversityHarbinPeople’s Republic of China

Personalised recommendations