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Journal of Forestry Research

, Volume 29, Issue 3, pp 631–638 | Cite as

Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination

  • Ling Yang
  • Dongyan Zhang
  • Hongnan Liu
  • Cheng Wei
  • Jianan Wang
  • Hailong Shen
Original Paper
  • 91 Downloads

Abstract

The effects of an exogenous nitric oxide donor (sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode (PTIO) and carboxy-PTIO potassium salt (cPTIO) on the embryo germination of Sorbus pohuashanensis were studied in a petri dish test. SNP at 0.5–5 mmol L−1 increased germination percentage, mean time to germination, germination index and germination energy compared with the control to different degrees. Treatment with 2 mmol L−1 SNP improved germination most significantly; embryo germination percentage for mother tree 1 (91.11%) and mother tree 2 (64.44%) were much higher than the control. In addition, excessive SNP levels did not enhance embryo germination. Combined treatment with SNP and an NO scavenger delayed embryo germination. Treatment with cPTIO inhibited embryo germination; germination percentage was 42.22% and was lower than that of the control. These results show that low concentrations of exogenous NO can enhance the embryo germination of S. pohuashanensis, providing a simple, effective way for promoting germination of S. pohuashanensis.

Keywords

Mountain ash Embryo germination Nitric oxide Sodium nitroprusside Nitric oxide scavenger 

Notes

Acknowledgements

We thank all the colleagues in our lab for constructive discussion and technical support.

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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Ling Yang
    • 1
  • Dongyan Zhang
    • 1
  • Hongnan Liu
    • 1
  • Cheng Wei
    • 1
  • Jianan Wang
    • 1
  • Hailong Shen
    • 1
  1. 1.State Key Laboratory of Forest Genetics and Breeding, School of ForestryNortheast Forestry UniversityHarbinPeople’s Republic of China

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