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Journal of Forestry Research

, Volume 29, Issue 4, pp 983–990 | Cite as

Screening chloroplast regions in Calophyllum brasiliense (Calophyllaceae) for suitability for population genetic analyses

  • Cecilia B. Percuoco
  • Lucas G. Giménez
  • Liliana N. Talavera Stéfani
  • Jorge V. Crisci
  • Carina F. Argüelles
Original Paper

Abstract

The uniparental inheritance and conserved structure of the chloroplast genome among many angiosperms makes it suitable for constructing gene lineages and inferring population histories based on these relationships. However, the analysis of a single chloroplast region to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast intergenic or intronic regions to select the most appropriate for studying the genetic variability in the chloroplast genome of a particular taxon. In this context, the aim of this work was to characterize 8 regions in Calophyllum brasiliense using 11 selected primer pairs and to evaluate their usefulness for phylogeographic analysis. Intergenic spacers petA-psbJ, petG-trnP and rpl32-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 regions will allow the analysis of genetic population structure and the integration of historical aspects to inform strategies for conserving C. brasiliense, especially for highly impacted populations and those at risk of local extinction.

Keywords

cpDNA cpSSRs Noncoding regions 

Notes

Acknowledgements

The present work was partially supported by Consejo de Investigación y Desarrollo Tecnológico (CIDET Project 16Q409 to C.F.A.) and Fondo para la Investigación Científica y Tecnológica Project (PICTO UNaM 2011 N°122 to C.F.A.). We also thank the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) for providing postgraduate fellowships to C.B.P. and L.N.T.S. and the Comité Ejecutivo de Desarrollo e Innovación Tecnológica (CEDIT) for the research fellowship to L.G.G. and L.N.T.S. and the Consejo Interuniversitario Nacional (CIN) for the fellowship to L.N.T.S.

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Copyright information

© Northeast Forestry University and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Cecilia B. Percuoco
    • 1
  • Lucas G. Giménez
    • 2
  • Liliana N. Talavera Stéfani
    • 1
  • Jorge V. Crisci
    • 3
  • Carina F. Argüelles
    • 1
  1. 1.Laboratorio GIGA, Instituto de Biología Subtropical, Nodo PosadasUniversidad Nacional de Misiones (UNaM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)PosadasArgentina
  2. 2.Cátedra de Genética Molecular, Facultad de Ciencias Exactas Químicas y Naturales (FCEQyN)Universidad Nacional de Misiones (UNaM)PosadasArgentina
  3. 3.Laboratorio de Sistemática y Biología Evolutiva (LASBE) - Facultad de Ciencias Naturales y Museo (FCNyM)Universidad Nacional de La Plata-(UNLP)La PlataArgentina

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