Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway

Abstract

Objective

To explore the protective effect and the underlying mechanism of Hu-Lu-Ba-Wan (葫芦巴丸, HLBW) on the testis of diabetic rats.

Methods

Twenty-four male Wistar rats (160–180 g) were randomly divided into 3 groups according to a random number table, including a control group (n=8), diabetic group (n=8), and HLBW group (n=8). Diabetic rat model was established by high-fat-diet administration and single intravenous injection of streptozotocin (26 mg/kg). Then HLBW granule was administrated for 12 weeks. Fasting blood glucose and insulin levels as well as serum total testosterone level and testicular testosterone content were examined. Oxidative stress markers in both serum and testis were tested. Meanwhile, testicular morphology was observed under hematoxylin and eosin (HE) and the ultrastructure of Leydig cell was observed by electron microscope. The superoxide anion level was detected by DHE, and TUNEL-positive cells of testis was evaluated by TUNEL assay. The gene and protein expression of protein kinase C (PKCα), phosphorylated PKCα (P-PKCα) and P47phox in testicular tissues were determined by quantitative RT-PCR analysis and Western bolt analysis.

Results

Compared with the diabetic group, HLBW treatment significantly reduced the fasting glucose levels and increased the levels of fasting insulin and testosterone in serum (P<0.01). HLBW administration also reduced the levels of reactive oxygen species (ROS) in plasma and alleviated the damage of oxidative stress in the testis of diabetic rats. Additionally, HLBW down-regulated the protein and mRNA levels of PKCα, P-PKCα and P47phox in testicular tissues.

Conclusion

HLBW may attenuate the oxidative stress in the testis of diabetic rats via PKCα /NAPDH oxidase signaling pathway.

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Authors

Corresponding author

Correspondence to Fu-er Lu.

Additional information

Supported by the National Natural Science Foundation of China (No. 81703886, 81273683)

Conflict of Interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

Author Contributions

Jiang SJ carried out the experiments, collected references and drafted the manuscript. Fang K and Li JB assisted Jiang SJ to finish the experiments. Chen G contributed to the statistical analysis. Lu FE defined the ideas of this study. Dong H designed the study and revised the manuscript. Xu LJ and Zou X provided the major support to study. All authors have read and approved the final manuscript.

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Jiang, Sj., Dong, H., Fang, K. et al. Protective Effects of Hu-Lu-Ba-Wan (葫芦巴丸) against Oxidative Stress in Testis of Diabetic Rats through PKCα/NAPDH Oxidase Signaling Pathway. Chin. J. Integr. Med. (2021). https://doi.org/10.1007/s11655-021-2863-2

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Keyworlds

  • Hu-Lu-Ba-Wan
  • Chinese medicine
  • oxidative stress
  • testis
  • diabetes
  • nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase