Shexiang Tongxin Dropping Pill (麝香通心滴丸) Protects against Na2S2O4-Induced Hypoxia-Reoxygenation Injury in H9c2 Cells

  • Shan Lin
  • Jiu-mao Lin
  • Ling Zhang
  • Da-xin Chen
  • Fei Xiao
  • Hong-wei Chen
  • You-qin Chen
  • Yu-ling Zhu
  • Jian-feng Chu
  • Jun Peng
Original Article
  • 8 Downloads

Abstract

Objectives

To investigate the protective effects of Shexiang Tongxin Dropping Pill (麝香通心滴丸, STP) on Na2S2O4-induced hypoxia-reoxygenation injury in cardiomyoblast H9c2 cells.

Methods

The cell viability and levels of mRNA and protein expression in H9c2 cells were determined following Na2S2O4-induced hypoxia using Hoechst staining, annexin V/propidium iodide (PI) flow cytometry, real-time polymerase chain reaction and Western blot analysis.

Results

STP pretreatment significantly increased the viability and inhibited aberrant morphological changes in H9c2 cardiomyoblast cells induced by Na2S2O4 treatment (P<0.05). In addition, STP pretreatment attenuated Na2S2O4-induced hypoxic damage, down-regulated the expression of pro-apoptotic Bax, and up-regulated the expression of anti-apoptotic Bcl-2 in H9c2 cells (P<0.05).

Conclusions

STP was strongly cardioprotective in hypoxia-reoxygenation injury by preventing hypoxic damage and inhibiting cellular apoptosis. These results further support the use of STP as an effective drug for the treatment of ischemic heart disease.

Keywords

myocardial ischemia apoptosis Shexiang Tongxin Dropping Pill Chinese medicine Na2S2O4-induced hypoxia-reoxygenation injury 

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Notes

Acknowledgements

The project was completed in Academy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine on Geriatric of Fujian University of Traditional Chinese Medicine. We thank Inner Mongolia Conba Pharmaceutical Co., Ltd. for providing STP and support.

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Copyright information

© Chinese Association of the Integration of Traditional and Western Medicine 2018

Authors and Affiliations

  • Shan Lin
    • 1
    • 2
    • 3
  • Jiu-mao Lin
    • 1
    • 2
  • Ling Zhang
    • 1
    • 2
  • Da-xin Chen
    • 1
    • 2
    • 3
  • Fei Xiao
    • 1
    • 2
  • Hong-wei Chen
    • 1
    • 2
    • 3
  • You-qin Chen
    • 4
  • Yu-ling Zhu
    • 5
  • Jian-feng Chu
    • 1
    • 2
    • 3
  • Jun Peng
    • 1
    • 2
    • 3
  1. 1.Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhouChina
  2. 2.Fujian Key Laboratory of Integrative Medicine on GeriatricFujian University of Traditional Chinese MedicineFuzhouChina
  3. 3.Rainbow Babies Children’s HospitalCase Western Reserve University School of MedicineClevelandUSA
  4. 4.Inner Mongolia Conba Pharmaceutical Co., Ltd.ShanghaiChina
  5. 5.CHEN Ke-ji Academic Thought Heritage StudioFuzhouChina

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