Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice
To evaluate the effect of Heyan Kuntai Capsule (和颜坤泰胶囊, HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes.
Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group), including the control group (saline), 17 β-estradiol group [E2, 100 μg/(kg·d)], and low-, medium-, and high-dose of HYKT groups [0.3, 0.9, 2.7 g/(kg·d), respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X (γH2AX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits, REC8 meiotic recombination protein (REC8), structural maintenance of chromosome (SMC) 1 β and SMC3 in oocytes were evaluated by immunofluorescent staining.
After administration of E2 and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P<0.05). Anti-γ H2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles (P<0.05). Furthermore, it showed an increased trend in the levels of REC8 and SMC1 β, after administration with E2 and HYKT, and no obvious change in the level of SMC3.
HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1 β) in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
Keywordsfollicular development cohesin oocyte Heyan Kuntai Capsule Chinese medicine
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- 26.Yang YF. RP-HPLC determination of paeonifl orin, baicalin, palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules. Chin Traditional Patent Med (Chin) 2010;32:958–960.Google Scholar
- 27.Li HF. Simultaneous determination of 6 components in Kuntai Capsule by RP-HPLC wavelength switching method. China Mod Med (Chin) 2015;22:7–11.Google Scholar
- 28.Zhang QY, Wang JW, Yu JM. Efficacy and safety of Kuntai Capsule and estrogen for menopausal syndrome: a metaanalysis. Natl Med J China (Chin) 2013;93:3445–3449.Google Scholar
- 29.Li CC, Wang JJ, Chen C, Li YF, Zheng QS, Yang J, et al. Treating menopause syndrome by Kuntai Capsule and hormone replacement therapy: a meta-analysis of efficacy and safety comparison. Chin J Integr Tradit West Med (Chin) 2013;33:1183–1190.Google Scholar
- 30.Zhang SF, Liu Y, Xie Q, Bi CP, Zhu HT, Ha LX, et al. Effects of Gengnian Ningxin Capsule on the histomorphology of ovarin and uterine in the menopausal rats. Chin J Geriatr (Chin) 2004;23:648–651.Google Scholar
- 31.Lian F, Jiang XY. Effect of Kuntai Capsule on the number of retrieved oocytes, high-quality oocytes and embryos in in vitro fertilization of poor ovarian response patients. Chin J Integr Tradit West Med (Chin) 2014;34:917–921.Google Scholar
- 32.Li WW, An LH, Zhang G, Wang XL, Sun LJ, Guan YC, et al. The Combined application of estradiol valerate-cyproterone acetate and Kuntai Capsule in poor ovarian response patients in vitro fertilization. J Pract Obestet Gynecol (Chin) 2014;30:681–685.Google Scholar
- 35.You SL, ed. Experimental zoology of obstetrics and gynecology. Beijing: Chinese Medicine Publisher of China;2007:7–8.Google Scholar
- 37.Skaznik-Wikiel ME, Mcguire MM, Sukhwani M, Donohue J, Chu T, Krivak TC, et al. Granulocyte colony-stimulating factor with or without stem cell factor extends time to premature ovarian insufficiency in female mice treated with alkylating chemotherapy. Fertil Steril 2013;99:2045–2054.CrossRefPubMedPubMedCentralGoogle Scholar
- 47.Zhang SF, Ha LX, Xie Q, Jiang J, Liu Y, Xia X, et al. Effect of Gengnian Ningxin Capsule and estradial valerate on splenocyte apoptosis gene Bcl-2 and Bax expression of menopausal rats. Chin J Integr Tradit West Med (Chin) 2004;24:201–204.Google Scholar
- 48.Cun-Cun L, Jing-Jing W, Chao C, Yun-Fei L, Qing-Shan Z, Juan Y, et al. Clinical efficacy of Kuntai Capsule compared with estrogen progesterone sequential treatment on perimenopausal syndrome and impact on life quality. Chin J Biochem Pharm (Chin) 2013;33:1183–1190.Google Scholar