Puerarin up-regulates methyl-CpG binding protein 2 phosphorylation in hippocampus of vascular dementia rats
- 12 Downloads
To observe the effect of puerarin on methyl-CpG binding protein 2 (MeCP2) phosphorylation (pMeCP2) in the hippocampus of a rat model of vascular dementia (VD).
Thirty-six healthy Sprague-Dawley rats were randomly assigned to the sham-operated group, dementia group and puerarintreated group using a random number table (n=12 per group). The modifified permanent bilateral common carotid artery occlusion method was used to establish the VD model. The sham-operated and dementia groups were given 2 mL/d of saline, while the puerarin-treated group was given 100 mg/(kg•d) of puerarin for 17 days. The learning and memory abilities were evaluated by the Morris water maze test. Hematoxylin-eosin staining, immunohistochemical (IHC) staining and Western blot analysis were carried out to observe changes in neuron morphology and in level of pMeCP2 in the hippocampus, respectively.
The morphologies of rat hippocampal neurons in the puerarintreated group were markedly improved compared with the dementia group. The escape latency of the dementia group was significantly longer than the sham-operated group (P<0.05), while the puerarin-treated group was obviously shorter than the dementia group (P<0.05). Cross-platform times of the dementia group were signifificantly decreased compared with the sham-operated group (P<0.05), while the puerarin-treated group was obviously increased compared with the dementia group (P<0.05). IHC staining showed no significant difference in the number of MeCP2 positive cells among 3 groups (P<0.05). The number of pMeCP2 positive cells in the CA1 region of hippocampus in the dementia group was signifificantly increased compared with the sham-operated group, and the puerarin-treated group was signifificantly increased compared with the dementia group (both P>0.05). Western blot analysis showed no signifificant difference of MeCP2 expression among 3 groups (P>0.05). The expression of pMeCP2 in the dementia group was signifificantly increased compared with the sham-operated group, while it in the puerarin-treated group was signifificantly increased compared with the dementia group (P<0.05).
Puerarin could play a role in the protection of nerve cells through up-regulating pMeCP2 in the hippocampus, improving neuron morphologies, and enhancing learning and memory ablities in a rat model of VD.
Keywordsvascular dementia puerarin methyl-CpG binding protein 2 phosphorylation
Unable to display preview. Download preview PDF.
- 1.Sun GH. A review on the clinical application of puerarin. Clin J Chin Med (Chin) 2010;2:100–101.Google Scholar
- 3.Kong H, Wang XQ, Wang QG, Zhao Y, Sun Y, Zhang Y, et al. Effect of puerarin on the pharmacokinetics of baicalin in Gegen Qinlian Decoction in mice. Chin J Integr Med 2015. [Epub ahead of print]Google Scholar
- 4.Wu HQ, Wang HQ, Zhang B, Zhang GL, Zhang R, Zhang LF. Puerarin decreases hypoxia inducible factor-1 alpha in the hippocampus of vascular dementia rats. Neur Regen Res 2012;7:421–425.Google Scholar
- 14.Li P, Zhou YY. Methyl CpG-binding protein 2 participating in the regulation of differentiation plasticity of nerve regeneration in the basal ganglia after ischemic stroke. Chin J Contemp Neurol Neurosurg (Chin) 2013;13:955–966.Google Scholar
- 17.Wang XH, Li LS. Comparison between the two methods of establishing 2VO model in rat. Acta Academ Med Milit Tertiae (Chin) 2002;24:1496.Google Scholar
- 18.Zhao J, Gou YJ, Peng XL, Yan W, Xie SP, Fan C, et al. Donepezil in the treatment of senile vascular dementia: a systematic review. Chin J Evid Based Med 2011;11:1280–1289.Google Scholar
- 22.Zhang BQ, Wang YL. Effcts of puerarin on hippocampal pyramidal cell and the expression of BDNF in vascular dementia rats. Chin J Neuroanat (Chin) 2007;23:615–620.Google Scholar