Dendrobium hybrids have been cultivated as commercially important ornamental plants in the floriculture industry. However, the potential of Dendrobium hybrids as a source of valuable secondary metabolites has not been fully exploited. In this study, the effect of various elicitors on the proliferation of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue and the corresponding production of dendrobine and anthocyanin was investigated. Yeast extract (YE) and glutamine (both at 200 mg L−1) increased dendrobine content in PLBs by 2.7- and 6.5-fold (54.874 ± 2.553 and 894.481 ± 26.185 ng mg−1dry weight (DW)) compared to controls (20.051 ± 0.182 and 136.956 ± 7.397 ng mg−1 DW). Glutamine (200 mg L−1) also significantly enhanced PLB biomass (0.190 ± 0.009 g) with respect to the control (0.144 ± 0.003 g). Chitosan and salicylic acid (SA) were not effective in enhancing the dendrobine and anthocyanin contents in PLBs. A combination of 4 mg L−1 NAA and 100 mg L−1 arginine increased dendrobine content by 8-fold (123.793 ± 5.446 ng mg−1 DW) compared to the control (15.554 ± 0.406 ng mg−1 DW). Anthocyanin content was increased by 1.7-fold (0.548 ± 0.019 Color Value (CV) g−1 DW) when PLBs treated with 4 mg L−1 TDZ were exposed to 1.5 h of UV irradiation as compared to those without UV treatment (0.331 ± 0.019 CV g−1 DW). Despite the low dendrobine content, PLBs as a source of dendrobine remains appealing due to its short culture duration (6 wk) compared to mother plants (2 yr).
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The authors would like to thank the Universiti Sains Malaysia Research University (USM-RU) Grant Scheme (1001/PBIOLOGI/811309) for supporting this study.
Conflict of interest
The authors declare no competing interests.
• To date, dendrobine and anthocyanins have not been reported in PLBs of Dendrobium species.
• In this study, PLBs of Dendrobium ‘Sabin Blue’ were used for producing dendrobine and anthocyanin compounds.
• This study aimed to establish an efficient PLB culture system of Dendrobium ‘Sabin Blue’ that can enhance the production of dendrobine and anthocyanin by using elicitors, amino acids, plant growth regulators (PGRs), and ultraviolet (UV) light exposure.
• Dendrobine and anthocyanin contents of PLBs, in vitro, and mother plants were compared to make a preliminary assessment of the enhancement strategies.
Editor: Praveen Saxena
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Chin, C.K., Stanly, C., Muniandy, A. et al. Protocorm-like bodies (PLBs) of Dendrobium Sabin Blue: a novel source for in vitro production of dendrobine and anthocyanin. In Vitro Cell.Dev.Biol.-Plant (2021). https://doi.org/10.1007/s11627-021-10159-3
- Plant growth regulators
- Amino acids
- Orchid tissue culture
- Secondary metabolites