Pteris aspericaulis var. tricolor T. Moore ex Lowe is a fern species with colorful fronds, potentially used as an ornamental plant. In the present study, we reported the establishment of in vitro regeneration of P. aspericaulis var. tricolor via green globular bodies (GGBs) system by using in vitro juvenile sporophytes as explants. GGB induction, multiplication and plantlet regeneration media, as well as the influence of light-emitting diodes (LEDs) on GGB multiplication and plantlet regeneration were investigated. The highest GGB induction frequency obtained on the medium supplemented with 2.73 μM thidiazuron (TDZ). The medium containing 1.32 μM 6-Benzyladenine (BA) was the optimum for GGB multiplication. GGB cultivated on 1/4 MS medium with the addition of 0.1% (w/v) activated charcoal (AC) behaved the highest differentiation frequency (94.44%), and 1/4 MS medium without AC was the most effective for plantlet growth. The light quality of LEDs (white, blue, red) had no effect on GGB multiplication and differentiation, but remarkably affected the plantlet height. Red LEDs promoted the petiole elongation, while blue LEDs inhibited it. Morphological and histological observation showed that GGBs consisted of a cluster of single GGBs with meristem and scale-like structure, and new single GGBs were efficiently initiated from the epidermal cells in top meristem of GGBs.
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Amaki W, Higuchi H (1991) A possible propagation system of Nephrolepis, Asplenium, Pteris, Adiantum and Rumohra (Arachniodes) through tissue culture. Acta Hortic 300:237–243
Amirchakhmaghi N, Hosseinpour B, Yousefzadeh H (2019) Development of a micropropagation protocol for Malus orientalis using axillary buds. In Vitro Cell Dev Bio - Plant 55:625–634. https://doi.org/10.1007/s11627-019-09992-4
Bertrand AM, Albuerne MA, Fernández H, González A, Sánchez-Tamés R (1999) In vitro organogenesis of Polypodium cambricum. Plant Cell, Tissue and Organ Cult 57:65–69. https://doi.org/10.1023/A:1006348628114
Camloha M, Gogala N, Rode J (1994) Plant regeneration from leaf explants of the fern Platycerium bifurcatum in vitro. Sci Hortic 56:257–266. https://doi.org/10.1016/0304-4238(94)90007-8
Chen X, Yang Q, Song W, Wang L, Guo W, Xue X (2017) Growth and nutritional properties of lettuce affected by different alternating intervals of red and blue LED irradiation. Sci Hortic 223:44–52. https://doi.org/10.1016/j.scienta.2017.04.037
Fernández H, Bertrand AM, Sáchez-Tamés R (1996) Micropropagation and phase change in Blechnum spicant and Pteris ensiformis. Plant cell, tissue and organ cult 44:261–265. https://doi.org/10.1007/BF00048534
Fernández H, Revilla MA (2003) In vitro culture of ornamental ferns. Plant Cell, Tissue and Organ Cult 73:1–13. https://doi.org/10.1023/A:1022650701341
Higuchi H, Amaki W (1989) Effects of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation. Sci Hortic 37:351–359. https://doi.org/10.1016/0304-4238(89)90146-5
Higuchi H, Amaki W, Suzuki S (1987) In vitro propagation of Nephrolepis cordifolia Prsel. Sci Hortic 32:105–113. https://doi.org/10.1016/0304-4238(87)90021-5
Hung CD, Hong C, Jung H, Kim S, Ket NV, Nam M, Choi D, Lee H (2015) Growth and morphogenesis of encapsulated strawberry shoot tips under mixed LEDs. Sci Hortic 194:94–200. https://doi.org/10.1016/j.scienta.2015.08.016
Jiang A, Zuo J, Zheng Q, Guo L, Gao L, Zhao S, Wang Q, Hu W (2019) Red LED irradiation maintains the postharvest quality of broccoli by elevating antioxidant enzyme activity and reducing the expression of senescence-related genes. Sci Hortic 251:73–79. https://doi.org/10.1016/j.scienta.2019.03.016
Jones DL (1987) Encyclopaedia of ferns. Timber press, Portland
Kher MM, Nataraj M (2019) Direct somatic embryogenesis and shoot regeneration from leaves and internodes of Pluchea lanceolata (DC.) C.B. Clarke. In vitro cell Dev bio - plant. https://doi.org/10.1007/s11627-019-10016-4
Khvatkov P, Chernobrovkina M, Okuneva A, Shvedova A, Chaban I, Dolgov S (2015) Callus induction and regeneration in Wolffia arrhiza (L.) Horkel ex Wimm. Plant cell. Tissue and Organ Cult 120:263–273. https://doi.org/10.1007/s11240-014-0624-z
Kim S, Hahn E, Heo J, Paek K (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143–151. https://doi.org/10.1016/j.scienta.2003.10.003
Kundu S, Gantait S (2018) Thidiazuron-induced protocorm-like bodies in orchid: progress and prospects. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 273–287
Li C, Xu Z, Dong R, Chang S, Wang L, Khalil-Ur-Rehman M, Tao J (2017) An RNA-seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red LED light, and white fluorescent light. Front Plant Sci 8:78. https://doi.org/10.3389/fpls.2017.00078
Li H, Xu Z, Tang C (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant cell. Tissue and Organ Cult 103:155–163
Lian M, Murthy HN, Paek K (2002) Effects of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid 'Pesaro'. Sci Hortic 94:365–370. https://doi.org/10.1016/S0304-4238(01)00385-5
Liao YK, Wu YH (2011) In vitro propagation of Platycerium bifurcatum (Cav.) C. Chr. Via green globular body initiation. Bot Stud 52:455–463
Liu M, Xu Z, Yang Y, Feng Y (2011) Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant cell, tissue and organ cult 106(1):1–10. https://doi.org/10.1007/s11240-010-9887-1
Lu SG (2017) An introduction of pteridology. Science Press, Beijing
Ma X, Wang Y, Liu M, Xu J, Xu Z (2015) Effects of green and red lights on the growth and morphogenesis of potato (Solanum tuberosum L.) plantlets in vitro. Sci Hortic 190:104–109. https://doi.org/10.1016/j.scienta.2015.01.006
Mikuła A, Pożoga M, Tomiczak K, Rybczyński J (2015) Somatic embryogenesis in ferns: a new experimental system. Plant Cell Rep 34:783–794. https://doi.org/10.1007/s00299-015-1741-9
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Murata T, Kadota A, Wada M (1997) Effects of blue light on cell elongation and microtubule orientation in dark-grown gametophytes of Ceratopteris richardii. Plant Cell Physiol 38:201–209. https://doi.org/10.1093/oxfordjournals.pcp.a029153
Ng CY, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies. Plant Cell, Tissue and Organ Cult 105(2):193–202. https://doi.org/10.1007/s11240-010-9851-0
Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue and Organ Cult 73:43–52. https://doi.org/10.1023/A:1022638508007
Ponni TG, Nair AS (2019) Somatic embryogenesis to overcome low seed viability and conserve wild banana (Ensete superbum (Roxb.) Cheesman). In Vitro Cell Dev Bio - Plant 55(4):371–379. https://doi.org/10.1007/s11627-019-09998-y
Seabrook JEA (2005) Light effects on the growth and morphogenesis of potato (Solanum tuberosum) in vitro: a review. Am J Potato Res 5(82):353–367
Soonthornkalump S, Nakkanong K, Meesawat U (2019) In vitro cloning via direct somatic embryogenesis and genetic stability assessment of Paphiopedilum niveum (Rchb.F.) stein: the endangered Venus's slipper orchid. In Vitro Cell & Dev Bio - Plant 55(3):265–276. https://doi.org/10.1007/s11627-019-09981-7
Teng WL (1997) Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant cell rep 17(2):77–83. https://doi.org/10.1007/s002990050356
Thakur RC, Hosoi Y, Ishii K (1998) Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro -an edible fern. Plant Cell Rep 18:203–208. https://doi.org/10.1007/s002990050557
Yu R, Zhang G, Li H, Mo X, Gui M, Zhou X, Jiang Y, Li S, Wang J (2017) In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies. Plant cell tissues organ cult 128:369–379. https://doi.org/10.1007/s11240-016-1116-0
Zhang XC (2012) Lycophytes and ferns of China. Peking University Press, Beijing
Zhou X, Gui M, Zhao D, Chen M, Ju S, Li S, Lu Z, Mo X, Wang J (2013) Study on reproductive barriers in 4x-2x crosses in Dianthus caryophyllus L. Euphytica 189:471–483. https://doi.org/10.1007/s10681-012-0819-5
We thank Shiliang Yang (Kunming University of Science and Technology) for his help in revising the paper.
This work was financially supported by the National Natural Science Foundations of China (Grant No. 31860569), the Science and Technology Talents and Platform Program of Yunnan Province - Rongpei Yu (2021-2026), the Science and Technology Talentsand Platform Program of Yunnan Province- Qinli Shan (Grant No. 2018HB117), Talent Introduction and Training Program of Yunnan Provincial Science and Technology Department (Grant No. 2016HA005).
Editor: Yong Eui Choi
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Yu, R., Li, F., Wang, G. et al. In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system. In Vitro Cell.Dev.Biol.-Plant (2021). https://doi.org/10.1007/s11627-020-10059-y
- Pteris aspericaulis var. tricolor
- In vitro regeneration
- Green globular bodies