In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system

Abstract

Pteris aspericaulis var. tricolor T. Moore ex Lowe is a fern species with colorful fronds, potentially used as an ornamental plant. In the present study, we reported the establishment of in vitro regeneration of P. aspericaulis var. tricolor via green globular bodies (GGBs) system by using in vitro juvenile sporophytes as explants. GGB induction, multiplication and plantlet regeneration media, as well as the influence of light-emitting diodes (LEDs) on GGB multiplication and plantlet regeneration were investigated. The highest GGB induction frequency obtained on the medium supplemented with 2.73 μM thidiazuron (TDZ). The medium containing 1.32 μM 6-Benzyladenine (BA) was the optimum for GGB multiplication. GGB cultivated on 1/4 MS medium with the addition of 0.1% (w/v) activated charcoal (AC) behaved the highest differentiation frequency (94.44%), and 1/4 MS medium without AC was the most effective for plantlet growth. The light quality of LEDs (white, blue, red) had no effect on GGB multiplication and differentiation, but remarkably affected the plantlet height. Red LEDs promoted the petiole elongation, while blue LEDs inhibited it. Morphological and histological observation showed that GGBs consisted of a cluster of single GGBs with meristem and scale-like structure, and new single GGBs were efficiently initiated from the epidermal cells in top meristem of GGBs.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Amaki W, Higuchi H (1991) A possible propagation system of Nephrolepis, Asplenium, Pteris, Adiantum and Rumohra (Arachniodes) through tissue culture. Acta Hortic 300:237–243

    Google Scholar 

  2. Amirchakhmaghi N, Hosseinpour B, Yousefzadeh H (2019) Development of a micropropagation protocol for Malus orientalis using axillary buds. In Vitro Cell Dev Bio - Plant 55:625–634. https://doi.org/10.1007/s11627-019-09992-4

    CAS  Article  Google Scholar 

  3. Bertrand AM, Albuerne MA, Fernández H, González A, Sánchez-Tamés R (1999) In vitro organogenesis of Polypodium cambricum. Plant Cell, Tissue and Organ Cult 57:65–69. https://doi.org/10.1023/A:1006348628114

    Article  Google Scholar 

  4. Camloha M, Gogala N, Rode J (1994) Plant regeneration from leaf explants of the fern Platycerium bifurcatum in vitro. Sci Hortic 56:257–266. https://doi.org/10.1016/0304-4238(94)90007-8

    Article  Google Scholar 

  5. Chen X, Yang Q, Song W, Wang L, Guo W, Xue X (2017) Growth and nutritional properties of lettuce affected by different alternating intervals of red and blue LED irradiation. Sci Hortic 223:44–52. https://doi.org/10.1016/j.scienta.2017.04.037

    CAS  Article  Google Scholar 

  6. Fernández H, Bertrand AM, Sáchez-Tamés R (1996) Micropropagation and phase change in Blechnum spicant and Pteris ensiformis. Plant cell, tissue and organ cult 44:261–265. https://doi.org/10.1007/BF00048534

    Article  Google Scholar 

  7. Fernández H, Revilla MA (2003) In vitro culture of ornamental ferns. Plant Cell, Tissue and Organ Cult 73:1–13. https://doi.org/10.1023/A:1022650701341

    Article  Google Scholar 

  8. Higuchi H, Amaki W (1989) Effects of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation. Sci Hortic 37:351–359. https://doi.org/10.1016/0304-4238(89)90146-5

    CAS  Article  Google Scholar 

  9. Higuchi H, Amaki W, Suzuki S (1987) In vitro propagation of Nephrolepis cordifolia Prsel. Sci Hortic 32:105–113. https://doi.org/10.1016/0304-4238(87)90021-5

    Article  Google Scholar 

  10. Hung CD, Hong C, Jung H, Kim S, Ket NV, Nam M, Choi D, Lee H (2015) Growth and morphogenesis of encapsulated strawberry shoot tips under mixed LEDs. Sci Hortic 194:94–200. https://doi.org/10.1016/j.scienta.2015.08.016

    Article  Google Scholar 

  11. Jiang A, Zuo J, Zheng Q, Guo L, Gao L, Zhao S, Wang Q, Hu W (2019) Red LED irradiation maintains the postharvest quality of broccoli by elevating antioxidant enzyme activity and reducing the expression of senescence-related genes. Sci Hortic 251:73–79. https://doi.org/10.1016/j.scienta.2019.03.016

    CAS  Article  Google Scholar 

  12. Jones DL (1987) Encyclopaedia of ferns. Timber press, Portland

    Google Scholar 

  13. Kher MM, Nataraj M (2019) Direct somatic embryogenesis and shoot regeneration from leaves and internodes of Pluchea lanceolata (DC.) C.B. Clarke. In vitro cell Dev bio - plant. https://doi.org/10.1007/s11627-019-10016-4

  14. Khvatkov P, Chernobrovkina M, Okuneva A, Shvedova A, Chaban I, Dolgov S (2015) Callus induction and regeneration in Wolffia arrhiza (L.) Horkel ex Wimm. Plant cell. Tissue and Organ Cult 120:263–273. https://doi.org/10.1007/s11240-014-0624-z

    CAS  Article  Google Scholar 

  15. Kim S, Hahn E, Heo J, Paek K (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Sci Hortic 101:143–151. https://doi.org/10.1016/j.scienta.2003.10.003

    Article  Google Scholar 

  16. Kundu S, Gantait S (2018) Thidiazuron-induced protocorm-like bodies in orchid: progress and prospects. In: Ahmad N, Faisal M (eds) Thidiazuron: from urea derivative to plant growth regulator. Springer, Singapore, pp 273–287

    Google Scholar 

  17. Li C, Xu Z, Dong R, Chang S, Wang L, Khalil-Ur-Rehman M, Tao J (2017) An RNA-seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red LED light, and white fluorescent light. Front Plant Sci 8:78. https://doi.org/10.3389/fpls.2017.00078

  18. Li H, Xu Z, Tang C (2010) Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant cell. Tissue and Organ Cult 103:155–163

  19. Lian M, Murthy HN, Paek K (2002) Effects of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid 'Pesaro'. Sci Hortic 94:365–370. https://doi.org/10.1016/S0304-4238(01)00385-5

    Article  Google Scholar 

  20. Liao YK, Wu YH (2011) In vitro propagation of Platycerium bifurcatum (Cav.) C. Chr. Via green globular body initiation. Bot Stud 52:455–463

    CAS  Google Scholar 

  21. Liu M, Xu Z, Yang Y, Feng Y (2011) Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant cell, tissue and organ cult 106(1):1–10. https://doi.org/10.1007/s11240-010-9887-1

    Article  Google Scholar 

  22. Lu SG (2017) An introduction of pteridology. Science Press, Beijing

    Google Scholar 

  23. Ma X, Wang Y, Liu M, Xu J, Xu Z (2015) Effects of green and red lights on the growth and morphogenesis of potato (Solanum tuberosum L.) plantlets in vitro. Sci Hortic 190:104–109. https://doi.org/10.1016/j.scienta.2015.01.006

    Article  Google Scholar 

  24. Mikuła A, Pożoga M, Tomiczak K, Rybczyński J (2015) Somatic embryogenesis in ferns: a new experimental system. Plant Cell Rep 34:783–794. https://doi.org/10.1007/s00299-015-1741-9

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

    CAS  Article  Google Scholar 

  26. Murata T, Kadota A, Wada M (1997) Effects of blue light on cell elongation and microtubule orientation in dark-grown gametophytes of Ceratopteris richardii. Plant Cell Physiol 38:201–209. https://doi.org/10.1093/oxfordjournals.pcp.a029153

    CAS  Article  Google Scholar 

  27. Ng CY, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies. Plant Cell, Tissue and Organ Cult 105(2):193–202. https://doi.org/10.1007/s11240-010-9851-0

    Article  Google Scholar 

  28. Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue and Organ Cult 73:43–52. https://doi.org/10.1023/A:1022638508007

    CAS  Article  Google Scholar 

  29. Ponni TG, Nair AS (2019) Somatic embryogenesis to overcome low seed viability and conserve wild banana (Ensete superbum (Roxb.) Cheesman). In Vitro Cell Dev Bio - Plant 55(4):371–379. https://doi.org/10.1007/s11627-019-09998-y

    CAS  Article  Google Scholar 

  30. Seabrook JEA (2005) Light effects on the growth and morphogenesis of potato (Solanum tuberosum) in vitro: a review. Am J Potato Res 5(82):353–367

    Article  Google Scholar 

  31. Soonthornkalump S, Nakkanong K, Meesawat U (2019) In vitro cloning via direct somatic embryogenesis and genetic stability assessment of Paphiopedilum niveum (Rchb.F.) stein: the endangered Venus's slipper orchid. In Vitro Cell & Dev Bio - Plant 55(3):265–276. https://doi.org/10.1007/s11627-019-09981-7

    CAS  Article  Google Scholar 

  32. Teng WL (1997) Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant cell rep 17(2):77–83. https://doi.org/10.1007/s002990050356

    CAS  Article  PubMed  Google Scholar 

  33. Thakur RC, Hosoi Y, Ishii K (1998) Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro -an edible fern. Plant Cell Rep 18:203–208. https://doi.org/10.1007/s002990050557

    CAS  Article  PubMed  Google Scholar 

  34. Yu R, Zhang G, Li H, Mo X, Gui M, Zhou X, Jiang Y, Li S, Wang J (2017) In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies. Plant cell tissues organ cult 128:369–379. https://doi.org/10.1007/s11240-016-1116-0

    CAS  Article  Google Scholar 

  35. Zhang XC (2012) Lycophytes and ferns of China. Peking University Press, Beijing

    Google Scholar 

  36. Zhou X, Gui M, Zhao D, Chen M, Ju S, Li S, Lu Z, Mo X, Wang J (2013) Study on reproductive barriers in 4x-2x crosses in Dianthus caryophyllus L. Euphytica 189:471–483. https://doi.org/10.1007/s10681-012-0819-5

    CAS  Article  Google Scholar 

Download references

Acknowledgments

We thank Shiliang Yang (Kunming University of Science and Technology) for his help in revising the paper.

Funding

This work was financially supported by the National Natural Science Foundations of China (Grant No. 31860569), the Science and Technology Talents and Platform Program of Yunnan Province - Rongpei Yu (2021-2026), the Science and Technology Talentsand Platform Program of Yunnan Province- Qinli Shan (Grant No. 2018HB117), Talent Introduction and Training Program of Yunnan Provincial Science and Technology Department (Grant No. 2016HA005).

Author information

Affiliations

Authors

Contributions

CY and QS collected spores and designed experiments. RY carried out experiments, analyzed data and wrote the manuscript. FL assisted in data analysis and manuscript revision. GW and JR assisted in establishment of GGB system. LW and MW assisted in plantlet acclimatization.

Corresponding authors

Correspondence to Chunmei Yang or Qinli Shan.

Additional information

Editor: Yong Eui Choi

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Yu, R., Li, F., Wang, G. et al. In vitro regeneration of the colorful fern Pteris aspericaulis var. tricolor via green globular bodies system. In Vitro Cell.Dev.Biol.-Plant (2021). https://doi.org/10.1007/s11627-020-10059-y

Download citation

Keywords

  • Pteris aspericaulis var. tricolor
  • In vitro regeneration
  • Green globular bodies
  • LEDs