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NMR-based metabolomics profile comparisons to distinguish between embryogenic and non-embryogenic callus tissue of sugarcane at the biochemical level

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Abstract

Nuclear magnetic resonance (NMR)-based metabolomics profile comparisons of embryogenic and non-embryogenic calli of sugarcane were performed using principal component analysis (PCA) to determine a possible relationship between certain metabolites and somatic embryogenesis. Mahalanobis distance (DM) analysis showed significant metabolic profile differences between the embryogenic and non-embryogenic callus groups. Significantly different spectral buckets and their corresponding metabolites have been identified using volcano- and loading-plot analyses, where glucose, fructose, sucrose, and alanine were observed at higher concentrations and asparagine, glutamine, lysine, 2-hydroxyisobutyrate, and choline were observed at lower concentrations in embryogenic calli than in non-embryogenic calli. The results of this research indicate possible roles of different sugars, amino acids, and aliphatic compounds during sugarcane somatic embryogenesis.

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Acknowledgments

We would like to thank Dr. Jack C. Comstock of USDA-ARS Sugarcane Field Station Canal Point, Florida for supplying the sugarcane materials used for initiating callus cultures that were used in this investigation as plant tissue materials for comparison. AB is supported by SC-INBRE (2 P20 GM103499), BS was supported by BlueCross BlueShield of South Carolina, and IM was supported by Biotechnology graduate program of Claflin University, South Carolina.

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Authors and Affiliations

  1. Department of Biology, Claflin University, 400 Magnolia St., Orangeburg, SC, 29115, USA

    Iqbal Mahmud & Kamal Chowdhury

  2. Department of Chemistry, Claflin University, Orangeburg, SC, 29115, USA

    Bhawana Shrestha & Arezue Boroujerdi

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  1. Iqbal Mahmud
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  2. Bhawana Shrestha
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  3. Arezue Boroujerdi
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  4. Kamal Chowdhury
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Correspondence to Kamal Chowdhury.

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Editor: David Duncan

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Mahmud, I., Shrestha, B., Boroujerdi, A. et al. NMR-based metabolomics profile comparisons to distinguish between embryogenic and non-embryogenic callus tissue of sugarcane at the biochemical level. In Vitro Cell.Dev.Biol.-Plant 51, 340–349 (2015). https://doi.org/10.1007/s11627-015-9687-8

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