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A novel long non-coding RNA, lncKBTBD10, involved in bovine skeletal muscle myogenesis

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Abstract

Accumulating evidence suggests that long non-coding RNAs (lncRNAs) play a crucial role in regulating skeletal muscle myogenesis, a highly coordinated multistep biological process. However, most studies of lncRNAs have focused on humans, mouse, and other model animals. In this study, we identified a novel lncRNA, named lncKBTBD10, located in the nucleus and involved in the proliferation and differentiation of bovine skeletal muscle satellite cells. Prediction of coding potential and in vitro translation system illustrated that lncKBTBD10 has no encoding capability. With the process of myogenic differentiation, the expression of lncKBTBD10 gradually increased. To elucidate the functions of lncKBTBD10 during myogenesis, the gain/loss-of-function experiments were performed. Results showed that the proliferation and differentiation of bovine skeletal muscle satellite cells were all suppressed whether lncKBTBD10 was knocked down or over-expressed. Furthermore, we found that lncKBTBD10 may affect its proximity gene KBTBD10 to involve in myogenesis. Results indicated that the protein level of KBTBD10 was all diminished after induced differentiation for 2 d in differentiation medium (DM2) whether lncKBTBD10 was knocked down or over-expressed. It may support why the altering of lncKBTBD10 can suppress the proliferation and differentiation of bovine skeletal muscle satellite cells. In short, our study elucidated that lncKBTBD10 could induce a decrease of KBTBD10 protein and further to affect bovine skeletal muscle myogenesis. The novel identified lncKBTBD10 may provide a reference for lncRNAs involved in myogenesis of bovine skeletal muscle.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (No.31572380) and the Natural Science Foundation of Tianjin City (No.15JCZDJC33700).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China

    Mingming Chen, Xin Li, Xiaojuan Zhang, Yan Li, Junxing Zhang, Minhui Liu, Linlin Zhang, Xiangbin Ding, Xinfeng Liu & Hong Guo

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  1. Mingming Chen
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  2. Xin Li
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  3. Xiaojuan Zhang
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  4. Yan Li
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  5. Junxing Zhang
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  7. Linlin Zhang
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  8. Xiangbin Ding
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  9. Xinfeng Liu
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  10. Hong Guo
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Correspondence to Xinfeng Liu or Hong Guo.

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Editor: Tetsuji Okamoto

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Chen, M., Li, X., Zhang, X. et al. A novel long non-coding RNA, lncKBTBD10, involved in bovine skeletal muscle myogenesis. In Vitro Cell.Dev.Biol.-Animal 55, 25–35 (2019). https://doi.org/10.1007/s11626-018-0306-y

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