Establishment and proteomic characterization of a novel synovial sarcoma cell line, NCC-SS2-C1
Synovial sarcoma is an aggressive mesenchymal tumor, characterized by the presence of unique transfusion gene, SS18–SSX. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of SS18–SSX in relevant cellular contexts. We report the establishment and proteomic characterization of a novel synovial sarcoma cell line. Primary tissue culture was performed using tumor tissue of synovial sarcoma. The established cell line was authenticated by assessing its DNA microsatellite short tandem repeat analysis and characterized by in vitro assay. Proteomic study was achieved by mass spectrometry, and the results were analyzed by treemap. The cell line NCC-SS2-C1 was established from a primary tumor tissue of a synovial sarcoma patient. The cell line has grown well for 11 mo and has been subcultured more than 15 times. The established cells were authenticated by assessing their short tandem repeat pattern comparing with that of original tumor tissue. The cells showed polygonal in shape and formed spheroid when seeded on the low-attachment dish. Proteomic analysis revealed the molecular pathways which are unique to the original tumor tissue or the established cell line. In conclusion, a novel synovial sarcoma cell line NCC-SS2-C1 was successfully established from the primary tumor tissue. The cell line has characteristic transfusion SS18–SSX and poses aggressive in vitro growth and capability of spheroid formation. Thus, NCC-SS2-C1 cell line will be a useful tool for investigation of the mechanisms of disease and the biological role of fusion gene.
KeywordsSynovial sarcoma Fusion gene Cell line Proteome
We thank Drs. Y. Minami, K. Shimizu, T. Mori, T. Uehara, M. Sugawara, Y. Araki, and Ms. R. Nakano (Division of Musculoskeletal Oncology, National Cancer Center Hospital) for sampling tumor tissue specimens from surgically resected materials. We would also like to thank Editage (www.editage.jp) for English language editing and constructive comments on the manuscript.
RO and TK prepared the manuscript. ST, ME, AY, and AK were involved in the analysis of the clinical and pathological data. RO, FK, MS, KS, and YT conducted the study. All authors read and approved the final manuscript.
This work was supported by the National Cancer Center Research and Development Fund (26-A-3, 26-A-9, and 29-A-2). The funding body financially supported the research.
Compliance with ethical standards
Ethics approval and consent to participate
This study was approved by the ethical committee of the National Cancer Center, and the patient in this study provided written informed consent.
Consent for publication
The authors declare that they have no competing interests.
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