Abstract
Although emerging data support crucial roles for microRNAs (miRNAs) during adipogenesis, the detailed mechanisms remain largely unknown. In this study, it was shown that in rabbits, levels of miR-148a-3p not only increased in white adipose tissue during early stages of growth but also during in vitro cultured preadipocyte differentiation. Furthermore, overexpression of miR-148a-3p significantly upregulated the mRNA levels of PPARγ, C/EBPα, and FABP4, as well as the protein levels of PPARγ, as indicated by qPCR and western blotting analyses. Overexpression of miR-148a-3p also promoted intracellular triglyceride accumulation. In contrast, downregulation of miR-148a-3p inhibited the differentiation of rabbit preadipocytes. Next, based on target gene prediction and a luciferase reporter assay, we further demonstrated that miR-148a-3p directly targeted one of the 3′ untranslated regions of PTEN. Finally, it was observed inhibition of PTEN by siRNA promoted rabbit preadipocyte differentiation. Taken together, our results suggested that miR-148a-3p could be involved in regulating rabbit preadipocyte differentiation through inhibiting expression of PTEN, which further highlighted the importance of miRNAs during adipogenesis.
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Abbreviations
- PPAR:
-
peroxisome proliferator-activated receptor
- C/EBP:
-
CCAAT/enhancer binding protein
- FABP4:
-
fatty acid-binding protein4
- PTEN:
-
tensin homolog
- PI3K:
-
phosphatidylinositol 3-kinase
- PIP 3:
-
phosphatidylinositol-3, 4, 5-trisphosphate
- GAPDH:
-
glyceraldehyde-3-phosphate dehydrogenase
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This work was supported by the earmarked fund for China Agriculture Research System (CARS-44-A-2).
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All experimental procedures using rabbits in our study were approved by the Institutional Animal Care and Use Committee of the College of Animal Science and Technology, Sichuan Agricultural University, China.
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The authors declare that they have no conflict of interest.
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Editor: Tetsuji Okamoto
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He, H., Cai, M., Zhu, J. et al. miR-148a-3p promotes rabbit preadipocyte differentiation by targeting PTEN. In Vitro Cell.Dev.Biol.-Animal 54, 241–249 (2018). https://doi.org/10.1007/s11626-018-0232-z
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DOI: https://doi.org/10.1007/s11626-018-0232-z