Labeling of adipose-derived stem cells with quantum dots provides stable and long-term fluorescent signal for ex vivo cell tracking
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Stem cells derived from adipose tissue (ADSC) have been used in cell therapy as an alternative to treat chronic and degenerative diseases. Using biomedical and image trials to track the cells when infused in the target tissue is essential to control cell migration and adhesion. The objective of the present study was to label and assess the adhesion of goat adipose tissue-derived stem cells (g-ADSC) after cell infusion in animal models by tracking luminescent intracytoplasmatic nanocrystals. The cells were labeled by using Qdots. The g-ADSCs infused with nanocrystal were prepared either fresh or fixed and further visualized under a fluorescence microscope. The labeled cells were infused in the goat mammary glands and mouse testicles and kidneys via tail vein injection. Thirty days after cell infusion, biopsy was carried out for analyses. The g-ADSC cultures were presented with high cellularity and fibroblast morphology, even after infusion of the nanocrystals. It was possible, by processing in paraffin and under fluorescence microscopy, demonstrating the success of the labeling in the long term. Freezing mammary gland biopsies in liquid NO2 did not alter the quality of labeling with Qdots. Therefore, g-ADSCs can be labeled with intracytoplasmatic nanocrystals (Qdots) enabling their in vitro and ex vivo tracking.
KeywordsQdots Adipose-derived stem cells Cell tracking Stem cells
We are thankful to the National Scientific and Technological Development Council-CNPq (Process: 552400/11-4; 311 684/2012-2) for their financial support. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Committee on the Ethics of Animal Experiments of the Federal Univesity of Piauí (Permit Number 037/2012).
- Barbash IM, Chouraqui P, Baron J, Feinberg MS, Etzion S, Tessone A, Miller L, Guetta E, Zipori D, Kedes LH, Kloner RA, Leor J (2003) Systemic delivery of bone marrow-derived mesenchymal stem cells to the infarcted myocardium: feasibility, cell migration, and body distribution. Circulation 108:863–868CrossRefPubMedGoogle Scholar
- Muccioli M, Pate M, Omosebi O, Benecia F (2011) Generation and labeling of murine bone marrow-derived dendritic cells with Qdot nanocrystals for tracking studies. J Vis Exp 52:1–5Google Scholar
- Oliveira DM, Almeida BO, Marti LC, Sibov TT, Pavon LF, Malheiros DMAC, Campos AH (2009) Labeling of human mesenchymal stem cells with QDs allows tracking of transplanted cells engrafted in infarcted pig hearts. Einstein 7:284–289Google Scholar
- Rosen AB, Kelly DJ, Schuldt AJ, Lu J, Potapova IA, Doronin SV, Robichaud KJ, Robinson RB, Rosen MR, Brink PR, Gaudette GR, Cohen IS (2007) Finding fluorescent needles in the cardiac haystack: tracking human mesenchymal stem cells labeled with QDs for quantitative in vivo three-dimensional fluorescence analysis. Stem Cell 25:2128–2138CrossRefGoogle Scholar
- Tognoli GK, Olsson DC, Martins DB, Santos Júnior EB, Salbego FZ, Oliveira GK, Braga FVA, Raiser AG, Dezengrini R, Cruz FS, Castro MB, Rosa MC, Carregaro AB, Pippi NL (2009) Transplante autólogo de células mononucleares da medula óssea em úlcera de córnea experimental em cães. Cienc Rural 39:148–155CrossRefGoogle Scholar
- Zimmerlin L (2010) Stromal vascular progenitors in adult human adipose tissue. Cytometry A 77A:22–30Google Scholar