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The influence of acute hypoxia on the functional and morphological state of the black scorpionfish red blood cells

  • Aleksandra Y. Andreyeva
  • Aleksander A. Soldatov
  • Vladimir S. Mukhanov
Article

Abstract

The investigation of the mechanisms of red blood cell steadiness to the oxygen lack in tolerant teleosts is of current scientific interest. Black scorpionfish, Scorpaena porcus L., is a widespread benthal species in the Black Sea and is highly resistant to hypoxic influence. The morphological state of black scorpionfish red blood cells under acute hypoxia was assessed using DNA-binding dye SYBR Green I and fluorescent microscopy. Changes in membrane potential of mitochondria and functional activity of cells were determined by rhodamine 123 (R123) and fluorescein diacetate (FDA) fluorescence. Oxygen deficiency leads to bidirectional changes in volume of erythrocytes and their nuclei. Between 0.57 and 1.76 mg О2 l−1, both parameters increased on 3–12 and 7–21%, respectively. At 1.76–4.03, cells shrank on 1.5–6.0% and nucleus size decreased on 1.5–3%. Acute hypoxia induced a significant increase of R123 (12–60%) and FDA (30–184%) fluorescence. These reactions are caused by a probable decrease in erythrocyte membrane permeability.

Keywords

Erythrocytes Flow cytometry Hypoxia Scorpaena porcus L. In vitro 

Notes

Acknowledgement

We thank James Loughran for his help in translating this article. Support from the Russian Fond of Fundamental Research (grant N16-04-00135) is kindly acknowledged.

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Copyright information

© The Society for In Vitro Biology 2016

Authors and Affiliations

  • Aleksandra Y. Andreyeva
    • 1
  • Aleksander A. Soldatov
    • 1
  • Vladimir S. Mukhanov
    • 2
  1. 1.Department of Animal Physiology and Biochemistry, Institute of Marine Biological ResearchRussian Academy of SciencesMoscowRussia
  2. 2.Department of Plankton, Institute of Marine Biological ResearchRussian Academy of SciencesMoscowRussia

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