Abstract
MSU-TnT4 (TnT4) cells, a newly established Trichoplusia ni cell line, was examined for the presence of a latent nodavirus, as had been described for another T. ni cell line, BTI-TN-5B1-4 (Hi5) cells. Reverse transcriptase polymerase chain reaction using nodavirus-specific primers did not detect virus genomic RNA in TnT4 cells. Transmission electron microscopy of recombinant baculovirus-infected TnT4 cells showed no evidence of latent nodavirus activation. Nodavirus particles were not detected in density gradients of baculovirus-infected TnT4 cell lysates or cell supernatants. The same methods confirmed the presence of a latent nodavirus in Hi5 cells.
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This work was supported in part by MSU grant TEC2008-0034. We thank Dr. Alicia Pastor for assistance with the electron microscopy.
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Editor: J. Denry Sato
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Zhang, F., Thiem, S.M. The Trichoplusia ni cell line MSU-TnT4 does not harbor a latent nodavirus. In Vitro Cell.Dev.Biol.-Animal 46, 1–6 (2010). https://doi.org/10.1007/s11626-009-9241-2
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DOI: https://doi.org/10.1007/s11626-009-9241-2