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Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody

Abstract

Nanoparticles conjugated with antibody were designed as active drug delivery system to reduce the toxicity and side effects of drugs for acute myeloid leukemia (AML). Moreover, methotrexate (MTX) was chosen as model drug and encapsulate within folic acid modified carboxymethyl chitosan (FA-CMCS) nanoparticles through self-assembling. The chemical structure, morphology, release and targeting of nanoparticles were characterized by routine detection. It is demonstrated that the mean diameter is about 150 nm, the release rate increases with the decreasing of pH, the binding rate of CD33 antibody and FA-CMCS nanoparticles is about 5:2, and nanoparticles can effectively bind onto HL60 cells in vitro. The experimental results indicate that the FA-CMCS nanoparticles conjugated with antibody may be used as a potential pHsensitive drug delivery system with leukemic targeting properties.

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Correspondence to Xueqiong Zhang or Han Yan.

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Funded by the National Natural Science Foundation of China (No. 50973088)

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Hu, Z., Zheng, H., Li, D. et al. Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 31, 446–453 (2016). https://doi.org/10.1007/s11595-016-1390-z

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Keywords

  • chitosan
  • nanoparticles
  • targeted drug delivery
  • cancer
  • controlled release
  • self-assembly
  • pH-sensitive