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Mycological Progress

, Volume 17, Issue 7, pp 833–840 | Cite as

Host species effects on bacterial communities associated with the fruiting bodies of Tuber species from the Sichuan Province in Southwest China

  • Lei Ye
  • Qiang Li
  • Yu Fu
  • Pierre Sourzat
  • Hao Tan
  • Jie Zou
  • Bo Zhang
  • Xiaolin Li
Original Article
  • 159 Downloads

Abstract

Tuber species produce highly sought-after truffles and host a wide diversity and high abundance of bacteria. It has been suggested that some of these bacteria contribute to the growth, maturity, and aromatic properties of truffles. Here, we characterized and compared the microbiomes of several species of truffles from the Sichuan Province in Southwest China using high-throughput sequencing of bacterial community 16S rRNA genes. Two T. pseudoexcavatum ascocarp samples had relatively similar bacterial communities, as indicated by PCoA analysis. In contrast, three T. indicum samples collected at different maturity stages did not contain similar communities, suggesting that the maturity stage of ascocarps affects community composition in addition to host phylogenetic background. Despite the variation seen among species and maturity stages, the Proteobacteria phylum dominated all communities, which is consistent with previous studies of Tuber-associated bacteria. Moreover, Bradyrhizobium was the dominant genus in most Tuber ascocarps, which is also consistent with previous studies, although the functional role of this genus within truffles is unclear. Notably, Serratia, which are essential producers of thiophene volatiles within T. borchii were dominant in all of our samples. This finding supports the hypothesis that the ability to produce thiophene volatiles is widespread among these bacteria.

Keywords

Tuber species Bacteria High-throughput sequencing Bradyrhizobium Serratia 

Notes

Acknowledgements

This work was supported by the Applied Basic Research Program of Sichuan Province (2015JY0088), Science and Technology Support Project in Sichuan Province (2016NYZ0040), Science and Technology Projects for Richening People and Strengthening County in Sichuan Province (Huidong County special), as well as the Sichuan Mushroom Innovation Team. We would like to thank LetPub (www.letpub.com) for providing linguistic assistance during the preparation of this manuscript.

Compliance with ethical standards

Conflict of interest statement

None declared.

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Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Soil and Fertilizer InstituteSichuan Academy of Agricultural SciencesChengduChina
  2. 2.College of Life ScienceSichuan UniversityChengduChina
  3. 3.Truffle Research Centre of Cahors-Le MontatLe MontatFrance

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