Mycological Progress

, Volume 17, Issue 3, pp 403–415 | Cite as

Simplified and efficient DNA extraction protocol for Meliolaceae specimens

  • Xiang-Yu Zeng
  • Rajesh Jeewon
  • Ting-Chi Wen
  • Sinang Hongsanan
  • Saranyaphat Boonmee
  • Kevin D. Hyde
Original Article


Meliolaceae is an obligate biotrophic fungal family which cannot be cultured in artificial media. This has resulted in a lack of DNA sequence data in public databases to better resolve species taxonomy. The main criterion for specific classification, therefore, relied heavily on host association. Fresh collections from living leaves of Croton persimilis and Tamarindus indica with black colonies in Mae Fah Luang University, Thailand yielded a new species, Irenopsis crotonicola sp. nov., and a new record of Meliola tamarindi. These taxa are described and phenotypic comparisons are made with known species. To better classify the putative novel species that cannot be cultivated, we outline the protocol to extract DNA from fruiting bodies and generate new phylogenetic data. The results indicate that this direct DNA extraction method is suitable to yield quality DNA sufficient for polymerase chain reaction (PCR) amplifications of several commonly used DNA regions in fungal systematics. The 28S rDNA phylogram generated confirms the position of our taxa within Meliolaceae and indicate a close relationship of I. crotonicola sp. nov. to I. walsurae. Sequences of tef, β-tubulin, and GPDH regions of Meliolaceae are provided as well.


Black mildews Meliolales Phylogeny Sordariomycetes 



This study was supported by the Science and Technology Foundation of Guizhou Province (no. [2012]3173). Rajesh Jeewon is grateful to Mae Fah Luang University for inviting him as a visiting professor and a keynote speaker for the COEIC 2017 conference. Kevin D. Hyde thanks the Chinese Academy of Sciences, project number 2013T2S0030, for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.


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Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Xiang-Yu Zeng
    • 1
    • 2
    • 3
  • Rajesh Jeewon
    • 4
  • Ting-Chi Wen
    • 1
  • Sinang Hongsanan
    • 2
  • Saranyaphat Boonmee
    • 2
  • Kevin D. Hyde
    • 2
    • 3
    • 5
  1. 1.The Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of EducationGuizhou UniversityGuiyangChina
  2. 2.Center of Excellence in Fungal ResearchMae Fah Luang UniversityChiang RaiThailand
  3. 3.School of ScienceMae Fah Luang UniversityChiang RaiThailand
  4. 4.Department of Health Sciences, Faculty of ScienceUniversity of MauritiusReduitMauritius
  5. 5.Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina

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