Advertisement

Mycological Progress

, Volume 17, Issue 3, pp 393–402 | Cite as

Colletotrichum atractylodicola sp. nov.: the anthracnose pathogen of Atractylodes chinensis in China

  • Haijiao Xu
  • Rujun Zhou
  • Junfan Fu
  • Yue Yuan
  • Xinxin Ge
  • Ulrike Damm
Original Article

Abstract

A new anthracnose disease of Atractylodes chinensis was observed in Liaoning province in China. The causal agent was isolated from diseased leaves. Based on morphology and ITS sequence data, it was identified as a Colletotrichum species belonging to the C. destructivum species complex. A multi-locus DNA sequence analysis (ITS, GAPDH, CHS-1, ACT, TUB2) revealed that the fungus represents a new species that is described here as C. atractylodicola sp. nov. Pathogenicity tests confirmed that the isolated species is the causal agent of the observed anthracnose symptoms on A. chinensis leaves.

Keywords

Atractylodes chinensis Colletotrichum atractylodicola New species Taxonomy 

References

  1. Armstrong-Cho C, Wang J, Wei Y, Banniza S (2012) The infection process of two pathogenic races of Colletotrichum truncatum on lentil. Can J Plant Pathol 34:58–67CrossRefGoogle Scholar
  2. Barimani M, Pethybridge SJ, Vaghefi N, Hay FS, Taylor PWJ (2013) A new anthracnose disease of pyrethrum caused by Colletotrichum tanaceti sp. nov. Plant Pathol 62:1248–1257CrossRefGoogle Scholar
  3. Cai L, Hyde KD, Taylor PWJ, Weir B, Waller JM, Abang MM, Zhang JZ, Yang YL, Phoulivong S, Liu ZY, Prihastuti H, Shivas RG, McKenzie EHC, Johnston PR (2009) A polyphasic approach for studying Colletotrichum. Fungal Divers 39:183–204Google Scholar
  4. Cannon PF, Damm U, Johnston PR, Weir BS (2012) Colletotrichum—current status and future directions. Stud Mycol 73:181–213CrossRefPubMedPubMedCentralGoogle Scholar
  5. Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556CrossRefGoogle Scholar
  6. Cho WD, Shin HD (2004) List of plant diseases in Korea, 4th edn. Korean Society of Plant Pathology, Suwon, 779 ppGoogle Scholar
  7. Choi KJ, Kim WG, Kim HG, Choi HW, Lee YK, Lee BD, Lee SY, Hong SK (2011) Morphology, molecular phylogeny and pathogenicity of Colletotrichum panacicola causing anthracnose of Korean ginseng. Plant Pathol J 27:1–7CrossRefGoogle Scholar
  8. Crous PW, Gams W, Stalpers JA, Robert V, Stegehuis G (2004) MycoBank: an online initiative to launch mycology into the 21st century. Stud Mycol 50:19–22Google Scholar
  9. Damm U, Woudenberg JHC, Cannon PF, Crous PW (2009) Colletotrichum species with curved conidia from herbaceous hosts. Fungal Divers 39:45–87Google Scholar
  10. Damm U, Cannon PF, Woudenberg JHC, Crous PW (2012a) The Colletotrichum acutatum species complex. Stud Mycol 73:37–113CrossRefPubMedPubMedCentralGoogle Scholar
  11. Damm U, Cannon PF, Woudenberg JHC, Johnston PR, Weir BS, Tan YP, Shivas RG, Crous PW (2012b) The Colletotrichum boninense species complex. Stud Mycol 73:1–36CrossRefPubMedPubMedCentralGoogle Scholar
  12. Damm U, Cannon PF, Liu F, Barreto RW, Guatimosim E, Crous PW (2013) The Colletotrichum orbiculare species complex: important pathogens of field crops and weeds. Fungal Divers 61:29–59CrossRefGoogle Scholar
  13. Damm U, O’Connell RJ, Groenewald JZ, Crous PW (2014) The Colletotrichum destructivum species complex—hemibiotrophic pathogens of forage and field crops. Stud Mycol 79:49–84CrossRefPubMedPubMedCentralGoogle Scholar
  14. Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772CrossRefPubMedPubMedCentralGoogle Scholar
  15. Duan J, Wang L, Qian S, Su S, Tang Y (2008) A new cytotoxic prenylated dihydrobenzofuran derivative and other chemical constituents from the rhizomes of Atractylodes lancea DC. Arch Pharm Res 31:965–969CrossRefPubMedGoogle Scholar
  16. Forseille L (2007) Molecular and pathological differentiation of Colletotrichum truncatum from scentless chamomile and legume crops. M.Sc. thesis, Biology Department, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, 122 ppGoogle Scholar
  17. Fu JF, Wang SW, Zhou RJ, Liu B (2014) Pathogen identification of yam anthracnose and its biological characteristics in Liaoning Province. J Jilin Agric Univ 36:395–400Google Scholar
  18. Gilbert RD, Johnson AM, Dean RA (1996) Chemical signals responsible for appressorium formation in the rice blast fungus Magnaporthe grisea. Physiol Mol Plant Pathol 48:335–346CrossRefGoogle Scholar
  19. Guerber JC, Liu B, Correll JC, Johnston PR (2003) Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95:872–895CrossRefPubMedGoogle Scholar
  20. Guo Y, Kondo K, Terabayashi S, Yamamoto Y, Shimada H, Fujita M, Kawasaki T, Maruyama T, Goda Y, Mizukami H (2006) DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region. J Nat Med 60:149–156CrossRefGoogle Scholar
  21. Honger JO, Offei SK, Oduro KA, Odamtten GT, Nyaku ST (2014) Identification and species status of the mango biotype of Colletotrichum gloeosporioides in Ghana. Eur J Plant Pathol 140:455–467CrossRefGoogle Scholar
  22. Hyde KD, Cai L, McKenzie EHC, Yang YL, Zhang JZ, Prihastuti H (2009) Colletotrichum: a catalogue of confusion. Fungal Divers 39:1–7Google Scholar
  23. Hyde KD, Chomnunti P, Crous PW, Groenewald JZ, Damm U, Ko Ko TW, Shivas RG, Summerell BA, Tan YP (2010) A case for re-inventory of Australia’s plant pathogens. Persoonia 25:50–60CrossRefPubMedPubMedCentralGoogle Scholar
  24. Kojima K, Kikuchi T, Takano Y, Oshiro E, Okuno T (2002) The mitogen-activated protein kinase gene MAF1 is essential for the early differentiation phase of appressorium formation in Colletotrichum lagenarium. Mol Plant Microbe Interact 15:1268–1276CrossRefPubMedGoogle Scholar
  25. Koval EZ (1961) New species of fungi of the Primorsky Krai. Ukrainian Bot J 18:73–80Google Scholar
  26. Li HX, Liu ZY, Wang JX, Zhou MG (2005) Baseline sensitivity of Colletotrichum gloeosporioides and C. capsici from capsium to azoxystrobin. Acta Phytopathol Sin 35:73–77Google Scholar
  27. Liu F, Cai L, Crous PW, Damm U (2013) Circumscription of the anthracnose pathogens Colletotrichum lindemuthianum and C. nigrum. Mycologia 105:844–860CrossRefPubMedGoogle Scholar
  28. Nakai Y, Sakakibara I, Hirakura K, Terabayashi S, Takeda S (2005) A new acetylenic compound from the rhizomes of Atractylodes chinensis and its absolute configuration. Chem Pharm Bull 53:1580–1581CrossRefPubMedGoogle Scholar
  29. Nirenberg HI (1976) Untersuchungen über die morphologische und biologische Differenzierung in der Fusarium—Sektion Liseola. Mitt Biol Bundesanst Land Forstwirtsch Berlin-Dahlem 169:1–117Google Scholar
  30. O’Connell RJ, Uronu AB, Waksman G, Nash C, Keon JPR, Bailey JA (1993) Hemibiotrophic infection of Pisum sativum by Colletotrichum truncatum. Plant Pathol 42:774–783CrossRefGoogle Scholar
  31. O’Connell RJ, Thon MR, Hacquard S et al (2012) Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. Nat Genet 44:1060–1065CrossRefPubMedGoogle Scholar
  32. O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116CrossRefPubMedGoogle Scholar
  33. Photita W, Taylor PWJ, Ford R, Hyde KD, Lumyong S (2005) Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Divers 18:117–133Google Scholar
  34. Phoulivong S, Cai L, Chen H, McKenzie EHC, Abdelsalam K, Chukeatirote E, Hyde KD (2010) Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Divers 44:33–43CrossRefGoogle Scholar
  35. Prihastuti H, Cai L, Chen H, McKenzie EHC, Hyde KD (2009) Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Divers 39:89–109Google Scholar
  36. Rannala B, Yang Z (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. J Mol Evol 43:304–311CrossRefPubMedGoogle Scholar
  37. Ren CG, Dai CC (2012) Jasmonic acid is involved in the signaling pathway for fungal endophyte-induced volatile oil accumulation of Atractylodes lancea plantlets. BMC Plant Biol 12:128CrossRefPubMedPubMedCentralGoogle Scholar
  38. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefPubMedGoogle Scholar
  39. Shiba M, Kondo K, Miki E, Yamaji H, Morota T, Terabayashi S, Takeda S, Sasaki H, Miyamoto KI, Aburada M (2006) Identification of medicinal Atractylodes based on ITS sequences of nrDNA. Biol Pharm Bull 29:315–320CrossRefPubMedGoogle Scholar
  40. Smith BJ, Black LL (1990) Morphological, cultural, and pathogenic variation among Colletotrichum species isolated from strawberry. Plant Dis 74:69–76CrossRefGoogle Scholar
  41. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690CrossRefPubMedGoogle Scholar
  42. Sutton BC (1992) The genus Glomerella and its anamorph Colletotrichum. In: Bailey JA, Jeger MJ (eds) Colletotrichum: biology, pathology and control. CAB International, Wallingford, pp 1–26Google Scholar
  43. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  44. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882CrossRefGoogle Scholar
  45. Uematsu S, Kageyama K, Moriwaki J, Sato T (2012) Colletotrichum carthami comb. nov., an anthracnose pathogen of safflower, garland chrysanthemum and pot marigold, revived by molecular phylogeny with authentic herbarium specimens. J Gen Plant Pathol 78:316–330CrossRefGoogle Scholar
  46. Walker J, Nikandrow A, Millar GD (1991) Species of Colletotrichum on Xanthium (Asteraceae) with comments on some taxonomic and nomenclatural problems in Colletotrichum. Mycol Res 95:1175–1193CrossRefGoogle Scholar
  47. Weir BS, Johnston PR, Damm U (2012) The Colletotrichum gloeosporioides species complex. Stud Mycol 73:115–180CrossRefPubMedPubMedCentralGoogle Scholar
  48. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar
  49. Wikee S, Cai L, Pairin N, McKenzie EHC, Su YY, Chukeatirote E, Thi HN, Bahkali AH, Moslem MA, Abdelsalam K, Hyde KD (2011) Colletotrichum species from jasmine (Jasminum sambac). Fungal Divers 46:171–182CrossRefGoogle Scholar
  50. Woudenberg JHC, Aveskamp MM, de Gruyter J, Spiers AG, Crous PW (2009) Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. Persoonia 22:56–62CrossRefPubMedPubMedCentralGoogle Scholar
  51. Yang YL, Liu ZY, Cai L, Hyde KD, Yu ZN, McKenzie EHC (2009) Colletotrichum anthracnose of Amaryllidaceae. Fungal Divers 39:123–146Google Scholar
  52. Yang YL, Cai L, Yu ZN, Liu ZY, Hyde KD (2011) Colletotrichum species on Orchidaceae in southwest China. Cryptogam Mycol 32:229–253CrossRefGoogle Scholar
  53. Zhaxybayeva O, Gogarten JP (2002) Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses. Genomics 3:4PubMedPubMedCentralGoogle Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Plant Pathology, College of Plant ProtectionShenyang Agricultural UniversityShenyangChina
  2. 2.Senckenberg Museum of Natural History GörlitzGörlitzGermany

Personalised recommendations