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Sequence variation of avirulence gene AVR-Pita1 in rice blast fungus, Magnaporthe oryzae

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Abstract

The interaction between rice, Oryza sativa, and rice blast fungus, Magnaporthe oryzae, is triggered by an interaction between the protein products of the host resistant gene, and the pathogen avirulence gene. This interaction follows the ‘gene-for-gene' concept. The resistant gene has effectively protected rice plants from rice blast infection. However, the resistant genes usually break down several years after the release of the resistant rice varieties because the fungus has evolved to new races. The objective of this study is to investigate the nucleotide sequence variation of the AVR-Pita1 gene that influences the adaption of rice blast fungus to overcome the resistant gene, Pi-ta. Thirty rice blast fungus isolates were collected in 2005 and 2010 from infected rice plants in northern and northeastern Thailand. The nucleotide sequences of AVR-Pita1 were amplified and analyzed. Phylogenetic analysis was conducted using the MEGA 5.0 program. The results showed a high level of nucleotide sequence polymorphisms and the positive genetic selection pressure in Thai rice blast isolates. The details of sequence variation analysis were described in this article. The information from this study can be used for rice blast resistant breeding program in the future.

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Acknowledgments

We thank Mr. A.J. Warner, Mr. N. Rasmi and Mr. W. Kaonongbua for critically and grammatically reviewing the manuscript. This research was supported by grants from Kasetsart University Research and Development Institute (KURDI), Faculty of Science and Graduate School, Kasetsart University and Ubon Ratchathani University.

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Authors and Affiliations

  1. Interdisciplinary Program in Genetic Engineering, Graduate School, Kasetsart University, Bangkok, 10900, Thailand

    Teerapong Kasetsomboon, Tanee Sriwongchai & Chatchawan Jantasuriyarat

  2. Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Teerapong Kasetsomboon & Chatchawan Jantasuriyarat

  3. Department of Agronomy, Faculty of Agriculture, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand

    Sureeporn Kate-Ngam

  4. Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand

    Tanee Sriwongchai

  5. Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    Bo Zhou

  6. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASTNAR, NRU-KU), Kasetsart University, Bangkok, 10900, Thailand

    Chatchawan Jantasuriyarat

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  1. Teerapong Kasetsomboon
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  2. Sureeporn Kate-Ngam
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Correspondence to Chatchawan Jantasuriyarat.

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Data deposition: The sequences reported in this paper have been deposited in the GenBank database (GenBank ID: JQ409300–JQ409329).

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Kasetsomboon, T., Kate-Ngam, S., Sriwongchai, T. et al. Sequence variation of avirulence gene AVR-Pita1 in rice blast fungus, Magnaporthe oryzae . Mycol Progress 12, 617–628 (2013). https://doi.org/10.1007/s11557-012-0867-1

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