The purpose of this study is to prepare nanoencapsulation synthesized with a new formulation of Fe@Au-CS-CU-FA nanoparticle (NPs) by pulsed laser ablation in liquid (PLAL) method as drug delivery to treat breast cancer (T-47D) and (MCF12A) as a normal cell line. The method synthesized Fe@Au NPs using the PLAL working at wavelength 532 nm with different laser fluence (1.9, 2.2, and 2.5) J/cm2. These Fe@Au NPs were characterized by atomic force microscope (AFM), field emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). The obtained mean sizes of Fe@Au NPs were 63.65, 32.47, and 31.18 nm at 1.9, 2.2, and 2.5 J/cm2, respectively. Results of the MTT assay of CU loaded Fe@Au-CS-FA NPs on human breast cancer cell line (T-47D) confirmed that cytotoxicity of CU can enhance when they are loaded on Fe@Au-CS-FA NPs in comparison with free CU. While results of flow cytometry showed that this combination can increase the therapeutic effects of CU by apoptosis induction in the T-47D cell line. Conclusion of Fe@Au-CS-CU-FA NPs causes a decrease in T-47D cell viability and caused induces 85% apoptosis. The in vivo study of Fe@Au-CS-CU-FA nanoformulation confirmed that the mean tumor size decreases in time.
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Al-Musawi S, Kadhim MJ, Hindi NKK (2018) Folated-nanocarrier for paclitaxel drug delivery in leukemia cancer therapy. J Pharm Sci Res 10(4):749–754
Al-Musawi S, Albukhaty S, Al-Karagoly H, Almalki F (2020) Design, and synthesis of multi-functional superparamagnetic core-gold shell coated with chitosan and folate nanoparticles for targeted antitumor therapy. Nanomat 11:1. https://doi.org/10.3390/nano11010032
Chen M, Yamamuro S, Farrell D, Majetich S (2003) Gold coated iron nanoparticles for biomedical applications. J Appl Phys 93:7551–7553
Al-Musawi S, Albukhaty S, Al-Karagoly H, Sulaiman GM, Alwahibi MS, Dewir YH, Soliman DA, Rizwana H (2020) Antibacterial activity of honey/chitosan nanofibers loaded with capsaicin and gold nanoparticles for wound dressing. Molecules 25:4770. https://doi.org/10.3390/molecules25204770
Al-Kinani MA, Haider AJ, Al-Musawi S (2020) Design, construction and characterization of intelligence polymer coated core–shell nanocarrier for curcumin drug encapsulation and delivery in lung cancer therapy purposes. J Inorg Organomet Polym. https://doi.org/10.1007/s10904-020-01672-w
Adawiya JH, Mohammad JH, Mohammad SM (2018) A review on preparation of silver nano-particles. J Amer Inst of Phys 1968:030086. https://doi.org/10.1063/1.5039273
Salih AA, Nazar A, Haider AJ (2019) Antibacterial activity of zno nanoparticle prepared by pulsed laser ablation in liquid for biological sensor. Proceedings - International Conference on Developments in eSystems Engineering, DeSE
Kadhim AA, Salman JAS, Haider AJ, Ibraheem SA, Kadhim HA (2019) Effect of zinc oxide nanoparticles biosynthesized by leuconostoc mesenteroides ssp. dextranicum against bacterial skin infections. Proceedings - International Conference on Developments in eSystems Engineering, DeSE
Goon IY, Lai LMH, Lim M, Munroe P, Gooding JJ, Amal R (2009) Fabrication and dispersion of gold-shell-protected magnetite nanoparticles: systematic control using polyethyleneimine. Chem Mater 21(4):673–681
Lingyan W et al (2005) Monodispersed core-shell Fe3O4@Au nanoparticles. J Phys Chem B 109:21593–21601
Alivisatos A (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271(5251):933–937
Averitt R, Sarkar D, Halas N (1997) Plasmon resonance shifts of Au coated Au2S nanoshells: insight into multicomponent nanoparticle growth. Phys Rev Lett 78(22):4217–4220
Baer R, Neuhauser D, Weiss S (2004) Enhanced absorption induced by a metallic nanoshell. Nano Lett 4(1):85–88
Kelly A, Lee-Ann J (2004) Bacterial separation and concentration from complex sample matrices: a review. Crit Rev Microbiol 30(1):7–24
Al-Musawi S, Hadi AJ, Hadi SJ, Hindi NKK (2019) Preparation and characterization of folated chitosan-magnetic nanocarrier for 5-fluorouracil drug delivery and studying its effect in bladder cancer therapy. J Global Pharma Tech 11(7):628–637
Al-Kinani MA, Haider AJ, Al-Musawi S (2020) High uniformity distribution of Fe@Au preparation by a micro-emulsion method. IOP Conf Ser: Mater Sci Eng 987:012013. https://doi.org/10.1088/1757-899X/987/1/012013
Qian W, Murakami M, Ichikawa Y, Che Y (2011) Highly efficient and controllable PEGylation of gold nanoparticles prepared by femtosecond laser ablation in water. Phys Chem C 115(47):23293–23298
Amendola V, Rizzi GA, Polizzi S, Meneghetti M (2005) Synthesis of gold nanoparticles by laser ablation in toluene: quenching and recovery of the surface plasmon absorption. Phys Chem B 109(49):23125–23128
Georgiou S, Koubenakis A (2003) Laser-induced material ejection from model molecular solids and liquids: mechanisms, implications, and applications. Chem Rev 103(2):349–393
Zhigilei L, Garrison B (1999) Mechanisms of laser ablation from molecular dynamics simulations: dependence of the initial temperature and pulse duration. Appl Phys A 69:S75–S80
Haibo Z, Xi-Wen D, Subhash C, Sergei A, Shikuan Y, Jianping H, Weiping C (2012a) Nanomaterials via laser ablation/irradiation in liquid: a review. Adv Funct Mater 22:1333–1353
Liu HY, Chen D, Li LL, Liu TL, Tan LF, Wu XL, Tang F (2011) Multifunctional gold nanoshells on silica nanorattles: a platform for the combination of photothermal therapy and chemotherapy with low systemic toxicity. Angew Chem 50:891–895
Ma M, Chen H, Chen Y, Wang X, Chen F, Cui X, Shi J (2012) Au capped magnetic core/mesoporous silica shell nanoparticles for combined photothermo-/chemo-therapy and multimodal imaging. Biomaterials 33:989–998
Hu M, Petrova H, Chen J, McLellan J, Siekkinen A, Marquez M, Xingde L, Younan X, Gregory V (2006) Ultrafast laser studies of the photothermal properties of gold nanocages. Phys Chem B 110:1520–1524
Haibo Z, Xi-Wen D, Subhash C, Sergei A, Shikuan Y, Jianping H, Weiping C (2012b) Nanomaterials via laser ablation/irradiation in liquid: a review. Adv Funct Mater 22(7):1333–1353
Guillermo G, Andres G, Luis M (2016) Reshaping, fragmentation, and assembly of gold nanoparticles assisted by pulse lasers. Acc Chem Res 49(4):678–686
Dongshi Z, Bilal G, Stephan B (2017) Laser synthesis and processing of colloids: fundamentals and applications. Chem Rev 117(5):3990–4103
Tatiana E (2010) On Nanoparticle formation by laser ablation in liquids. Phys Chem C 115(12):5044–5048
Karamipour S, Sadjadi M, Farhadyar N (2015) Fabrication and spectroscopic studies of folic acid-conjugated Fe3O4@Au core-shell for targeted drug delivery application. Spectrochim Acta Part A: Mol Biomol Spectrosc 148:146–155
Philipp W, Jurij J, Christoph R, Venkata S, Claas T, Ulf W, Mathias B, Lorenz K, Stephan B (2016) Solvent-surface interactions control the phase structure in lasergenerated iron-gold core-shell nanoparticles. Sci Rep 6(1)
Albukhaty, S.; Al-Musawi, S.; Abdul Mahdi, S.; Sulaiman, G.M.; Alwahibi, M.S.; Dewir, Y.H.; Soliman, D.A.; Rizwana, H. (2020) Investigation of Dextran-coated superparamagnetic nanoparticles for targeted vinblastine controlled release, delivery, apoptosis induction, and gene expression in pancreatic cancer cells. Molecules 25:4721. https://doi.org/10.3390/molecules25204721
Ma’mani L, Nikzad S, Kheiri-Manjili H, Al-Musawi S, Saeedi M, Askarlou S, Foroumadi A, Shafiee A (2014) Curcumin-loaded guanidine functionalized PEGylated I3ad mesoporous silica nanoparticles KIT-6: practical strategy for the breast cancer therapy. Eur J Med Chem 18(83):646–654. https://doi.org/10.1016/j.ejmech.2014.06.069
Al-Awady MJ, Balakit AA, Al-Musawi S, Alsultani MJ, Kamil Ahmed, Alabbasi M (2019) Investigation of anti-MRSA and anticancer activity of eco-friendly synthesized silver nanoparticles from palm dates extract. Nano Biomed Eng 11(2):157–169. https://doi.org/10.5101/nbe.v11i2.p157-169
Mofazzal Jahromi, M., Al-Musawi, S., Pirestani, M., Fasihi Ramandi, M., Ahmadi, K., Rajayi, H., Mohammad Hassan, Z., Kamali, M., Mirnejad, R. (2014) Curcumin-loaded chitosan tripolyphosphate nanoparticles as a safe, natural and effective antibiotic inhibits the infection of Staphylococcus aureus and Pseudomonas aeruginosa in vivo. Iran J Biotech 12(3):e1012. https://doi.org/10.15171/ijb.1012
The authors wish to thank the University of Technology and Genetic Engineering Department, Al-Qasim Green University (Babylon/Iraq), in Iraq for providing the research facilities.
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Al-Kinani, M.A., Haider, A.J. & Al-Musawi, S. Design and Synthesis of Nanoencapsulation with a New Formulation of Fe@Au-CS-CU-FA NPs by Pulsed Laser Ablation in Liquid (PLAL) Method in Breast Cancer Therapy: In Vitro and In Vivo. Plasmonics (2021). https://doi.org/10.1007/s11468-021-01371-3
- Fe@Au NPs
- Au shell
- Fe core
- Breast cancer
- Drug delivery