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Genome-wide distribution of histone H3 acetylation in all-trans retinoic acid induced neuronal differentiation of SH-SY5Y cells

  • Articles/Molecular Biology
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Chinese Science Bulletin

Abstract

With chromatin immunoprecipitation (ChIP) and promoter DNA microarray analyses (ChIP-on-chip), we analyzed the variations of acetylation on histone H3 in all-trans retinoic acid (RA) induced neuronal cell differentiation. Neuroblastoma SH-SY5Y cells were treated with RA for 24 h and the acetylation on histone H3 in the promoter region of the genes was detected. Results showed that, after treatment, the level of acetylation on histone H3 elevated in 597 genes in the genome, and reduced in the other 647 genes compared with those of the control. In summary, we have successfully adopted a high throughput technique to detect and analyze variations of acetylation of histone H3 in human genome at the early phage of RA induced neuronal differentiation of the SH-SY5Y cells.

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Authors and Affiliations

  1. National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China

    HongBo Fang, Yang Mi, NingHua Wu, Ye Zhang & YuFei Shen

Authors
  1. HongBo Fang
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  2. Yang Mi
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  3. NingHua Wu
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  4. Ye Zhang
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  5. YuFei Shen
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Corresponding authors

Correspondence to Ye Zhang or YuFei Shen.

Additional information

Supported by National Natural Science Foundation of China (Grant Nos. 90408007 and 30721063) and National Key Basic Research and Development Program of China (Grant No. 2004CB518605)

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Fang, H., Mi, Y., Wu, N. et al. Genome-wide distribution of histone H3 acetylation in all-trans retinoic acid induced neuronal differentiation of SH-SY5Y cells. Chin. Sci. Bull. 54, 1043–1049 (2009). https://doi.org/10.1007/s11434-009-0109-3

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  • DOI: https://doi.org/10.1007/s11434-009-0109-3

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