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Transpositional behaviour of the Ds element in the Ac/Ds system in rice

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  • Genetics
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Chinese Science Bulletin

Abstract

Insertional mutagenesis based on maize Activator/Dissociator (Ac/Ds) transposons is becoming a major approach used to produce a saturated mutant collection in rice. In this research, Ds-T-DNA transformed homozygotes were crossed with Ac-T-DNA transformed homozygotes in order to establish an Ac/Ds transposon system in rice. The successive investigation of Ds transposition from F1 to F5 generations indicated that the frequencies of germinal transposition increased over successive generations and reached 54.2% in F3 generation. The Ds transposition pattern revealed that a Ds transposition induced an approximately 170-bp deletion of T-DNA sequence and another Ds transposition carried a 272-bp T-DNA sequence. Using thermal asymmetric interlaced PCR (TAIL-PCR), some flanking sequences of the Ds element were amplified. Analyses of 17 Ds-flanking sequences showed that five Ds were inserted into gene regions. The Ds could transpose not only to the linked sites but also to the unlinked sites. The frequency of inter-chromosomal transposition of Ds was 33.3%.

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Authors and Affiliations

  1. College of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Liu Fang, Zhang XiangQian, Zhang ZeMin, Chen ZhaoGui, Zhu HaiTao & Zhang GuiQuan

  2. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, Shanghai, 200032, China

    Wang Jiang & Zhang JingLiu

Authors
  1. Liu Fang
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  2. Zhang XiangQian
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  3. Zhang ZeMin
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  4. Chen ZhaoGui
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  5. Zhu HaiTao
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  6. Wang Jiang
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  7. Zhang JingLiu
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  8. Zhang GuiQuan
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Corresponding author

Correspondence to Zhang GuiQuan.

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Contributed equally to this work

Supported by National Basic Research Program of China (Grant No. G19990116)

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Liu, F., Zhang, X., Zhang, Z. et al. Transpositional behaviour of the Ds element in the Ac/Ds system in rice. CHINESE SCI BULL 52, 2789–2796 (2007). https://doi.org/10.1007/s11434-007-0415-6

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  • DOI: https://doi.org/10.1007/s11434-007-0415-6

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