Quantification of SARS-CoV-2 antigen levels in the blood of patients with COVID-19


  1. Bavishi, C., Bonow, R.O., Trivedi, V., Abbott, J.D., Messerli, F.H., and Bhatt, D.L. (2020). Acute myocardial injury in patients hospitalized with COVID-19 infection: A review. Prog Cardiovasc Dis https://doi.org/10.1016/j.pcad.2020.05.013.

  2. Gabarre, P., Dumas, G., Dupont, T., Darmon, M., Azoulay, E., and Zafrani, L. (2020). Acute kidney injury in critically ill patients with COVID-19. Intens Care Med 46, 1339–1348.

    CAS  Article  Google Scholar 

  3. Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., Zhang, L., Fan, G., Xu, J., Gu, X., et al. (2020). Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet 395, 497–506.

    CAS  Article  Google Scholar 

  4. Lippi, G., Mattiuzzi, C., Bovo, C., and Plebani, M. (2020). Current laboratory diagnostics of coronavirus disease 2019 (COVID-19). Acta Biomed 91, 137–145.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Mak, G.C., Cheng, P.K., Lau, S.S., Wong, K.K., Lau, C.S., Lam, E.T., Chan, R.C., and Tsang, D.N. (2020). Evaluation of rapid antigen test for detection of SARS-CoV-2 virus. J Clin Virol 129, 104500.

    CAS  Article  Google Scholar 

  6. Phipps, M.M., Barraza, L.H., LaSota, E.D., Sobieszczyk, M.E., Pereira, M. R., Zheng, E.X., Fox, A.N., Zucker, J., and Verna, E.C. (2020). Acute liver injury in COVID-19: prevalence and association with clinical outcomes in a large U.S. cohort. Hepatology 72, 807–817.

    CAS  Article  Google Scholar 

  7. Rissin, D.M., and Walt, D.R. (2006). Digital concentration readout of single enzyme molecules using femtoliter arrays and poisson statistics. Nano Lett 6, 520–523.

    CAS  Article  Google Scholar 

  8. Rissin, D.M., Kan, C.W., Campbell, T.G., Howes, S.C., Fournier, D.R., Song, L., Piech, T., Patel, P.P., Chang, L., Rivnak, A.J., et al. (2010). Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations. Nat Biotechnol 28, 595–599.

    CAS  Article  Google Scholar 

  9. Scohy, A., Anantharajah, A., Bodéus, M., Kabamba-Mukadi, B., Verroken, A., and Rodriguez-Villalobos, H. (2020). Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis. J Clin Virol 129, 104455.

    CAS  Article  Google Scholar 

  10. Yuan, C., Zhu, H., Yang, Y., Cai, X., Xiang, F., Wu, H., Yao, C., Xiang, Y., and Xiao, H. (2020). Viral loads in throat and anal swabs in children infected with SARS-CoV-2. Emerg Microb Infect 9, 1233–1237.

    CAS  Article  Google Scholar 

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The authors gratefully appreciated the participation of patients in this study. We thank BingXu and Junhui Li for their excellent assistance for sample preparation and data processing. This work was supported by the Ministry of Science and Technology of the People’s Republic of China (2020YFC0844900, 2020YFC0841700, and 2020YFC0848700), the National Natural Science Foundation of China (81470566, 81670765, 81772165, and 81974303), the China Primary Health Care Foundation-Youan Medical Development Fund (BJYAYY-2020PY-01) and the funds from Neoline, Gbio and Quanterix. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Correspondence to Liangzhi Xie or Ronghua Jin or Yingmei Feng.

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The author(s) declare that they have no conflict of interest. This study and all the relevant experiments were approved by the Beijing Youan Hospital Research Ethics Committee, and written informed consent was obtained from each participant in accordance with the Declaration of Helsinki.

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Su, B., Yin, J., Lin, X. et al. Quantification of SARS-CoV-2 antigen levels in the blood of patients with COVID-19. Sci. China Life Sci. (2020). https://doi.org/10.1007/s11427-020-1830-8

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